MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.

<h4>Background</h4>MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5' seed region of miRNAs is believed to be essential for the...

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Autores principales:	Yukari Takahashi, Alistair R R Forrest, Emi Maeno, Takehiro Hashimoto, Carsten O Daub, Jun Yasuda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11c8bf5079da43c0828fdec925d172fd
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spelling	oai:doaj.org-article:11c8bf5079da43c0828fdec925d172fd2021-11-25T06:20:57ZMiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.1932-620310.1371/journal.pone.0006677https://doaj.org/article/11c8bf5079da43c0828fdec925d172fd2009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19688090/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5' seed region of miRNAs is believed to be essential for the specific suppression of target gene expression. One miRNA can have several hundred different targets in a cell. Rapidly accumulating evidence suggests that many miRNAs are involved in cell cycle regulation and consequentially play critical roles in carcinogenesis.<h4>Methodology/principal findings</h4>Introduction of synthetic miR-107 or miR-185 suppressed growth of the human non-small cell lung cancer cell lines. Flow cytometry analysis revealed these miRNAs induce a G1 cell cycle arrest in H1299 cells and the suppression of cell cycle progression is stronger than that by Let-7 miRNA. By the gene expression analyses with oligonucleotide microarrays, we find hundreds of genes are affected by transfection of these miRNAs. Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6.<h4>Conclusions/significance</h4>We identified new cell cycle regulating miRNAs, miR-107 and miR-185, localized in frequently altered chromosomal regions in human lung cancers. Especially for miR-107, a large number of down-regulated genes are annotated with the gene ontology term 'cell cycle'. Our results suggest that these miRNAs may contribute to regulate cell cycle in human malignant tumors.Yukari TakahashiAlistair R R ForrestEmi MaenoTakehiro HashimotoCarsten O DaubJun YasudaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 8, p e6677 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yukari Takahashi Alistair R R Forrest Emi Maeno Takehiro Hashimoto Carsten O Daub Jun Yasuda MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
description	<h4>Background</h4>MicroRNAs (miRNAs) are short single stranded noncoding RNAs that suppress gene expression through either translational repression or degradation of target mRNAs. The annealing between messenger RNAs and 5' seed region of miRNAs is believed to be essential for the specific suppression of target gene expression. One miRNA can have several hundred different targets in a cell. Rapidly accumulating evidence suggests that many miRNAs are involved in cell cycle regulation and consequentially play critical roles in carcinogenesis.<h4>Methodology/principal findings</h4>Introduction of synthetic miR-107 or miR-185 suppressed growth of the human non-small cell lung cancer cell lines. Flow cytometry analysis revealed these miRNAs induce a G1 cell cycle arrest in H1299 cells and the suppression of cell cycle progression is stronger than that by Let-7 miRNA. By the gene expression analyses with oligonucleotide microarrays, we find hundreds of genes are affected by transfection of these miRNAs. Using miRNA-target prediction analyses and the array data, we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6.<h4>Conclusions/significance</h4>We identified new cell cycle regulating miRNAs, miR-107 and miR-185, localized in frequently altered chromosomal regions in human lung cancers. Especially for miR-107, a large number of down-regulated genes are annotated with the gene ontology term 'cell cycle'. Our results suggest that these miRNAs may contribute to regulate cell cycle in human malignant tumors.
format	article
author	Yukari Takahashi Alistair R R Forrest Emi Maeno Takehiro Hashimoto Carsten O Daub Jun Yasuda
author_facet	Yukari Takahashi Alistair R R Forrest Emi Maeno Takehiro Hashimoto Carsten O Daub Jun Yasuda
author_sort	Yukari Takahashi
title	MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
title_short	MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
title_full	MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
title_fullStr	MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
title_full_unstemmed	MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
title_sort	mir-107 and mir-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/11c8bf5079da43c0828fdec925d172fd
work_keys_str_mv	AT yukaritakahashi mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines AT alistairrrforrest mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines AT emimaeno mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines AT takehirohashimoto mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines AT carstenodaub mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines AT junyasuda mir107andmir185caninducecellcyclearrestinhumannonsmallcelllungcancercelllines
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MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines.

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