25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ

Neonatal infectious pneumonia (NIP) is a common infectious disease that develops in the neonatal period. The purpose of our study was to explore the potential roles of 25(OH)-Vitamin D (25-OH-VD) and its anti-inflammatory mechanism in NIP. The results showed that serum 25-OH-VD level was negatively...

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Autores principales:	Qi Sun, Yiwen Gao, Lina Qiao, Yi Yuan, Qian Liu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	neonatal infectious pneumonia (nip) 25-oh-vd inflammatory indicators deficiency yap/taz anti-inflammatory mechanism Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/11cc157ba1774bca8695bf4a66362b3c
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spelling	oai:doaj.org-article:11cc157ba1774bca8695bf4a66362b3c2021-11-04T15:51:53Z25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ2165-59792165-598710.1080/21655979.2021.1990000https://doaj.org/article/11cc157ba1774bca8695bf4a66362b3c2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1990000https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Neonatal infectious pneumonia (NIP) is a common infectious disease that develops in the neonatal period. The purpose of our study was to explore the potential roles of 25(OH)-Vitamin D (25-OH-VD) and its anti-inflammatory mechanism in NIP. The results showed that serum 25-OH-VD level was negatively correlated with the severity of NIP, whereas Spearman’s correlation analysis showed a significant positive correlation between the severity of NIP and the levels of pneumonia markers procalcitonin (PCT) and interleukin-6 (IL-6). The expression of vitamin D receptor (VDR) was down-regulated, while the transforming growth factor β (TGFβ), nuclear YAP, and TAZ were up-regulated in the peripheral blood mononuclear cells (PBMCs) of neonates with severe pneumonia. Neonates with 25-OH-VD deficiency were associated with an increased risk of NIP. In BEAS-2B cells, down-regulation of nuclear YAP and TAZ was found in the lipopolysaccharide (LPS) + VD group relative to the LPS-induced group. Additionally, positive rate of nuclear YAP, as detected by immunocytochemistry (ICC), and the nuclear translocation of nuclear YAP/TAZ by IFA in the LPS+VD group showed an intermediate level between that of the control and LPS-induced groups. Furthermore, the expressions of VDR and CYP27B1 were significantly increased in the LPS+VD group as compared to those in the LPS-induced group. The anti-inflammatory mechanism in NIP was achieved due to the 25-OH-VD mediating TGFβ/YAP/TAZ pathway, which suggested that using 25-OH-VD might be a potential strategy for NIP treatment.Qi SunYiwen GaoLina QiaoYi YuanQian LiuTaylor & Francis Grouparticleneonatal infectious pneumonia (nip)25-oh-vdinflammatory indicatorsdeficiencyyap/tazanti-inflammatory mechanismBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8931-8942 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neonatal infectious pneumonia (nip) 25-oh-vd inflammatory indicators deficiency yap/taz anti-inflammatory mechanism Biotechnology TP248.13-248.65
spellingShingle	neonatal infectious pneumonia (nip) 25-oh-vd inflammatory indicators deficiency yap/taz anti-inflammatory mechanism Biotechnology TP248.13-248.65 Qi Sun Yiwen Gao Lina Qiao Yi Yuan Qian Liu 25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ
description	Neonatal infectious pneumonia (NIP) is a common infectious disease that develops in the neonatal period. The purpose of our study was to explore the potential roles of 25(OH)-Vitamin D (25-OH-VD) and its anti-inflammatory mechanism in NIP. The results showed that serum 25-OH-VD level was negatively correlated with the severity of NIP, whereas Spearman’s correlation analysis showed a significant positive correlation between the severity of NIP and the levels of pneumonia markers procalcitonin (PCT) and interleukin-6 (IL-6). The expression of vitamin D receptor (VDR) was down-regulated, while the transforming growth factor β (TGFβ), nuclear YAP, and TAZ were up-regulated in the peripheral blood mononuclear cells (PBMCs) of neonates with severe pneumonia. Neonates with 25-OH-VD deficiency were associated with an increased risk of NIP. In BEAS-2B cells, down-regulation of nuclear YAP and TAZ was found in the lipopolysaccharide (LPS) + VD group relative to the LPS-induced group. Additionally, positive rate of nuclear YAP, as detected by immunocytochemistry (ICC), and the nuclear translocation of nuclear YAP/TAZ by IFA in the LPS+VD group showed an intermediate level between that of the control and LPS-induced groups. Furthermore, the expressions of VDR and CYP27B1 were significantly increased in the LPS+VD group as compared to those in the LPS-induced group. The anti-inflammatory mechanism in NIP was achieved due to the 25-OH-VD mediating TGFβ/YAP/TAZ pathway, which suggested that using 25-OH-VD might be a potential strategy for NIP treatment.
format	article
author	Qi Sun Yiwen Gao Lina Qiao Yi Yuan Qian Liu
author_facet	Qi Sun Yiwen Gao Lina Qiao Yi Yuan Qian Liu
author_sort	Qi Sun
title	25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ
title_short	25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ
title_full	25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ
title_fullStr	25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ
title_full_unstemmed	25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ
title_sort	25(oh)-vitamin d alleviates neonatal infectious pneumonia via regulating tgfβ-mediated nuclear translocation mechanism of yap/taz
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/11cc157ba1774bca8695bf4a66362b3c
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25(OH)-Vitamin D alleviates neonatal infectious pneumonia via regulating TGFβ-mediated nuclear translocation mechanism of YAP/TAZ

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