Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

Abstract Although sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA in the regulation of this process is still not clarified. The tiger pufferfish (Takifugu rubripes) is one of the most economically important marine cultured species i...

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Autores principales:	Hongwei Yan, Qi Liu, Jieming Jiang, Xufang Shen, Lei Zhang, Zhen Yuan, Yumeng Wu, Ying Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11d39e001d164b06be1c881c05443fbc
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spelling	oai:doaj.org-article:11d39e001d164b06be1c881c05443fbc2021-12-02T14:23:18ZIdentification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads10.1038/s41598-021-83891-w2045-2322https://doaj.org/article/11d39e001d164b06be1c881c05443fbc2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83891-whttps://doaj.org/toc/2045-2322Abstract Although sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA in the regulation of this process is still not clarified. The tiger pufferfish (Takifugu rubripes) is one of the most economically important marine cultured species in Asia, but analyses of miRNA and long non-coding RNA (lncRNA) at early sex differentiation stages have not been conducted yet. In our study, high-throughput sequencing technology was used to sequence transcriptome libraries from undifferentiated gonads of T. rubripes. In total, 231 (107 conserved, and 124 novel) miRNAs were obtained, while 2774 (523 conserved, and 2251 novel) lncRNAs were identified. Of these, several miRNAs and lncRNAs were predicted to be the regulators of the expression of sex-related genes (including fru-miR-15b/foxl2, novel-167, novel-318, and novel-538/dmrt1, novel-548/amh, lnc_000338, lnc_000690, lnc_000370, XLOC_021951, and XR_965485.1/gsdf). Analysis of differentially expressed miRNAs and lncRNAs showed that three mature miRNAs up-regulated and five mature miRNAs were down-regulated in male gonads compared to female gonads, while 79 lncRNAs were up-regulated and 51 were down-regulated. These findings could highlight a group of interesting miRNAs and lncRNAs for future studies and may reveal new insights into the function of miRNAs and lncRNAs in sex determination and differentiation.Hongwei YanQi LiuJieming JiangXufang ShenLei ZhangZhen YuanYumeng WuYing LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongwei Yan Qi Liu Jieming Jiang Xufang Shen Lei Zhang Zhen Yuan Yumeng Wu Ying Liu Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads
description	Abstract Although sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA in the regulation of this process is still not clarified. The tiger pufferfish (Takifugu rubripes) is one of the most economically important marine cultured species in Asia, but analyses of miRNA and long non-coding RNA (lncRNA) at early sex differentiation stages have not been conducted yet. In our study, high-throughput sequencing technology was used to sequence transcriptome libraries from undifferentiated gonads of T. rubripes. In total, 231 (107 conserved, and 124 novel) miRNAs were obtained, while 2774 (523 conserved, and 2251 novel) lncRNAs were identified. Of these, several miRNAs and lncRNAs were predicted to be the regulators of the expression of sex-related genes (including fru-miR-15b/foxl2, novel-167, novel-318, and novel-538/dmrt1, novel-548/amh, lnc_000338, lnc_000690, lnc_000370, XLOC_021951, and XR_965485.1/gsdf). Analysis of differentially expressed miRNAs and lncRNAs showed that three mature miRNAs up-regulated and five mature miRNAs were down-regulated in male gonads compared to female gonads, while 79 lncRNAs were up-regulated and 51 were down-regulated. These findings could highlight a group of interesting miRNAs and lncRNAs for future studies and may reveal new insights into the function of miRNAs and lncRNAs in sex determination and differentiation.
format	article
author	Hongwei Yan Qi Liu Jieming Jiang Xufang Shen Lei Zhang Zhen Yuan Yumeng Wu Ying Liu
author_facet	Hongwei Yan Qi Liu Jieming Jiang Xufang Shen Lei Zhang Zhen Yuan Yumeng Wu Ying Liu
author_sort	Hongwei Yan
title	Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads
title_short	Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads
title_full	Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads
title_fullStr	Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads
title_full_unstemmed	Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads
title_sort	identification of sex differentiation-related microrna and long non-coding rna in takifugu rubripes gonads
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/11d39e001d164b06be1c881c05443fbc
work_keys_str_mv	AT hongweiyan identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT qiliu identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT jiemingjiang identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT xufangshen identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT leizhang identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT zhenyuan identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT yumengwu identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads AT yingliu identificationofsexdifferentiationrelatedmicrornaandlongnoncodingrnaintakifugurubripesgonads
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Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads

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