Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout

Abstract Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, has extensive negative effects on wild and farmed salmonids worldwide. Vaccination induces some protection under certain conditions but disease outbreaks occur even in vaccinated fish. Therefore, alternative dise...

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Autores principales:	Moonika H. Marana, Asma M. Karami, Jørgen Ødegård, Shaozhi Zuo, Rzgar M. Jaafar, Heidi Mathiessen, Louise von Gersdorff Jørgensen, Per W. Kania, Inger Dalsgaard, Torben Nielsen, Kurt Buchmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11d487049bb0497ab52e1130f3849695
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spelling	oai:doaj.org-article:11d487049bb0497ab52e1130f38496952021-12-02T18:03:05ZWhole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout10.1038/s41598-021-97437-72045-2322https://doaj.org/article/11d487049bb0497ab52e1130f38496952021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97437-7https://doaj.org/toc/2045-2322Abstract Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, has extensive negative effects on wild and farmed salmonids worldwide. Vaccination induces some protection under certain conditions but disease outbreaks occur even in vaccinated fish. Therefore, alternative disease control approaches are required to ensure the sustainable expansion of rainbow trout aquaculture. Selective breeding can be applied to enhance host resistance to pathogens. The present work used genome-wide association study (GWAS) to identify quantitative trait loci (QTL) associated with A. salmonicida resistance in rainbow trout. A total 798 rainbow trout exposed to A. salmonicida by bath challenge revealed 614 susceptible and 138 resistant fish. Genotyping was conducted using the 57 K single nucleotide polymorphism (SNP) array and the GWAS was performed for survival and time to death phenotypes. We identified a QTL on chromosome 16 and located positional candidate genes in the proximity of the most significant SNPs. In addition, samples from exposed fish were examined for expression of 24 immune-relevant genes indicating a systematic immune response to the infection. The present work demonstrated that resistance to A. salmonicida is moderately heritable with oligogenic architecture. These result will be useful for the future breeding programs for improving the natural resistance of rainbow trout against furunculosis.Moonika H. MaranaAsma M. KaramiJørgen ØdegårdShaozhi ZuoRzgar M. JaafarHeidi MathiessenLouise von Gersdorff JørgensenPer W. KaniaInger DalsgaardTorben NielsenKurt BuchmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moonika H. Marana Asma M. Karami Jørgen Ødegård Shaozhi Zuo Rzgar M. Jaafar Heidi Mathiessen Louise von Gersdorff Jørgensen Per W. Kania Inger Dalsgaard Torben Nielsen Kurt Buchmann Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout
description	Abstract Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, has extensive negative effects on wild and farmed salmonids worldwide. Vaccination induces some protection under certain conditions but disease outbreaks occur even in vaccinated fish. Therefore, alternative disease control approaches are required to ensure the sustainable expansion of rainbow trout aquaculture. Selective breeding can be applied to enhance host resistance to pathogens. The present work used genome-wide association study (GWAS) to identify quantitative trait loci (QTL) associated with A. salmonicida resistance in rainbow trout. A total 798 rainbow trout exposed to A. salmonicida by bath challenge revealed 614 susceptible and 138 resistant fish. Genotyping was conducted using the 57 K single nucleotide polymorphism (SNP) array and the GWAS was performed for survival and time to death phenotypes. We identified a QTL on chromosome 16 and located positional candidate genes in the proximity of the most significant SNPs. In addition, samples from exposed fish were examined for expression of 24 immune-relevant genes indicating a systematic immune response to the infection. The present work demonstrated that resistance to A. salmonicida is moderately heritable with oligogenic architecture. These result will be useful for the future breeding programs for improving the natural resistance of rainbow trout against furunculosis.
format	article
author	Moonika H. Marana Asma M. Karami Jørgen Ødegård Shaozhi Zuo Rzgar M. Jaafar Heidi Mathiessen Louise von Gersdorff Jørgensen Per W. Kania Inger Dalsgaard Torben Nielsen Kurt Buchmann
author_facet	Moonika H. Marana Asma M. Karami Jørgen Ødegård Shaozhi Zuo Rzgar M. Jaafar Heidi Mathiessen Louise von Gersdorff Jørgensen Per W. Kania Inger Dalsgaard Torben Nielsen Kurt Buchmann
author_sort	Moonika H. Marana
title	Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout
title_short	Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout
title_full	Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout
title_fullStr	Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout
title_full_unstemmed	Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout
title_sort	whole-genome association study searching for qtl for aeromonas salmonicida resistance in rainbow trout
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/11d487049bb0497ab52e1130f3849695
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Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout

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