Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins

Abstract Saponins from defatted root-extract of Securidaca longipedunculata were systematically entrapped in emulsion monolayer-barrier and finally recovered in pure form through demulsification. First, their molecules were dispersed in water to engineer a monomolecular film architecture, via self-a...

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Autores principales:	Titus C. Obasi, Radu Moldovan, Anca Toiu, Cornelia Braicu, Ede Bodoki, Ioana Berindan-Neagoe, Ilioara Oniga, Robert Sandulescu, Radu Oprean
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11dd6f8db9da4ffa98dcf0dd7a439b40
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spelling	oai:doaj.org-article:11dd6f8db9da4ffa98dcf0dd7a439b402021-12-02T15:04:57ZMolecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins10.1038/s41598-017-15067-42045-2322https://doaj.org/article/11dd6f8db9da4ffa98dcf0dd7a439b402017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-15067-4https://doaj.org/toc/2045-2322Abstract Saponins from defatted root-extract of Securidaca longipedunculata were systematically entrapped in emulsion monolayer-barrier and finally recovered in pure form through demulsification. First, their molecules were dispersed in water to engineer a monomolecular film architecture, via self-assembly. Emulsifying with ethyl-ether resulted in swollen micelles and engendered phase-inversion and phase-separation, by disrupting the thermodynamic equilibrium. As positive outcome, a Winsor II system was obtained, having saponin-rich upper phase (ethyl-ether) and impurities bound lower phase (aqueous). Saponin particles underwent transition in insoluble ethyl-ether, precipitated and recovered as solids. The entire process was bioactivity-guided and validated using pooled fractions of securidaca saponins, purified by TLC (RP-C18, F254S). TEM and SEM revealed interesting morphologies and particle sizes between nanometer and micron. At the end, purity output of 90% and total recovery of 94% were achieved. Here we show that “molecular-trapping in emulsion’s monolayer” is an effective method for recovery, production and purification of saponins of plant origin.Titus C. ObasiRadu MoldovanAnca ToiuCornelia BraicuEde BodokiIoana Berindan-NeagoeIlioara OnigaRobert SandulescuRadu OpreanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Titus C. Obasi Radu Moldovan Anca Toiu Cornelia Braicu Ede Bodoki Ioana Berindan-Neagoe Ilioara Oniga Robert Sandulescu Radu Oprean Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins
description	Abstract Saponins from defatted root-extract of Securidaca longipedunculata were systematically entrapped in emulsion monolayer-barrier and finally recovered in pure form through demulsification. First, their molecules were dispersed in water to engineer a monomolecular film architecture, via self-assembly. Emulsifying with ethyl-ether resulted in swollen micelles and engendered phase-inversion and phase-separation, by disrupting the thermodynamic equilibrium. As positive outcome, a Winsor II system was obtained, having saponin-rich upper phase (ethyl-ether) and impurities bound lower phase (aqueous). Saponin particles underwent transition in insoluble ethyl-ether, precipitated and recovered as solids. The entire process was bioactivity-guided and validated using pooled fractions of securidaca saponins, purified by TLC (RP-C18, F254S). TEM and SEM revealed interesting morphologies and particle sizes between nanometer and micron. At the end, purity output of 90% and total recovery of 94% were achieved. Here we show that “molecular-trapping in emulsion’s monolayer” is an effective method for recovery, production and purification of saponins of plant origin.
format	article
author	Titus C. Obasi Radu Moldovan Anca Toiu Cornelia Braicu Ede Bodoki Ioana Berindan-Neagoe Ilioara Oniga Robert Sandulescu Radu Oprean
author_facet	Titus C. Obasi Radu Moldovan Anca Toiu Cornelia Braicu Ede Bodoki Ioana Berindan-Neagoe Ilioara Oniga Robert Sandulescu Radu Oprean
author_sort	Titus C. Obasi
title	Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins
title_short	Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins
title_full	Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins
title_fullStr	Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins
title_full_unstemmed	Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins
title_sort	molecular-trapping in emulsion’s monolayer: a new strategy for production and purification of bioactive saponins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/11dd6f8db9da4ffa98dcf0dd7a439b40
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Molecular-trapping in Emulsion’s Monolayer: A New Strategy for Production and Purification of Bioactive Saponins

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