Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes

Abstract Confocal Raman spectroscopy (CRS) can provide information about oocyte competency through measurement of changes in the macromolecular architecture during oocyte development and maturation. Hitherto most spectroscopic studies have been limited to fixed oocytes due to the inherent difficulti...

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Autores principales:	Philip Heraud, Katarzyna Maria Marzec, Qing‒Hua Zhang, Wai Shan Yuen, John Carroll, Bayden R. Wood
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11ddf29393e94723aff040e434d0454c
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spelling	oai:doaj.org-article:11ddf29393e94723aff040e434d0454c2021-12-02T11:53:04ZLabel-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes10.1038/s41598-017-08973-02045-2322https://doaj.org/article/11ddf29393e94723aff040e434d0454c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08973-0https://doaj.org/toc/2045-2322Abstract Confocal Raman spectroscopy (CRS) can provide information about oocyte competency through measurement of changes in the macromolecular architecture during oocyte development and maturation. Hitherto most spectroscopic studies have been limited to fixed oocytes due to the inherent difficulties working with live cells. Here we report the first three-dimensional images of living murine oocytes using CRS. We show that fixation induces significant changes in the macromolecular chemistry compared to living oocytes. A band at 1602 cm−1, assigned to a marker for mitochondria function was found in living oocytes but absent from fixed oocytes providing an in vivo marker. Fixation resulted in significant changes in protein and nucleic acid bands and the spatial distribution of organelles. Raman imaging of Metaphase I and II (MI, MII) and germinal vesicle stage oocytes showed changes in nuclear organisation and cytoplasm macromolecular architecture during these development and maturation stages related to changes in chromosome condensation, mitochondria aggregation and lipid droplet numbers.Philip HeraudKatarzyna Maria MarzecQing‒Hua ZhangWai Shan YuenJohn CarrollBayden R. WoodNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Philip Heraud Katarzyna Maria Marzec Qing‒Hua Zhang Wai Shan Yuen John Carroll Bayden R. Wood Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes
description	Abstract Confocal Raman spectroscopy (CRS) can provide information about oocyte competency through measurement of changes in the macromolecular architecture during oocyte development and maturation. Hitherto most spectroscopic studies have been limited to fixed oocytes due to the inherent difficulties working with live cells. Here we report the first three-dimensional images of living murine oocytes using CRS. We show that fixation induces significant changes in the macromolecular chemistry compared to living oocytes. A band at 1602 cm−1, assigned to a marker for mitochondria function was found in living oocytes but absent from fixed oocytes providing an in vivo marker. Fixation resulted in significant changes in protein and nucleic acid bands and the spatial distribution of organelles. Raman imaging of Metaphase I and II (MI, MII) and germinal vesicle stage oocytes showed changes in nuclear organisation and cytoplasm macromolecular architecture during these development and maturation stages related to changes in chromosome condensation, mitochondria aggregation and lipid droplet numbers.
format	article
author	Philip Heraud Katarzyna Maria Marzec Qing‒Hua Zhang Wai Shan Yuen John Carroll Bayden R. Wood
author_facet	Philip Heraud Katarzyna Maria Marzec Qing‒Hua Zhang Wai Shan Yuen John Carroll Bayden R. Wood
author_sort	Philip Heraud
title	Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes
title_short	Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes
title_full	Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes
title_fullStr	Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes
title_full_unstemmed	Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes
title_sort	label-free in vivo raman microspectroscopic imaging of the macromolecular architecture of oocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/11ddf29393e94723aff040e434d0454c
work_keys_str_mv	AT philipheraud labelfreeinvivoramanmicrospectroscopicimagingofthemacromoleculararchitectureofoocytes AT katarzynamariamarzec labelfreeinvivoramanmicrospectroscopicimagingofthemacromoleculararchitectureofoocytes AT qinghuazhang labelfreeinvivoramanmicrospectroscopicimagingofthemacromoleculararchitectureofoocytes AT waishanyuen labelfreeinvivoramanmicrospectroscopicimagingofthemacromoleculararchitectureofoocytes AT johncarroll labelfreeinvivoramanmicrospectroscopicimagingofthemacromoleculararchitectureofoocytes AT baydenrwood labelfreeinvivoramanmicrospectroscopicimagingofthemacromoleculararchitectureofoocytes
_version_	1718394850639872000

Label-free in vivo Raman microspectroscopic imaging of the macromolecular architecture of oocytes

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