Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi

Abstract This is the first study in an aquatic ectotherm to combine a stoichiometric bioenergetic approach with an endpoint stochastic model to explore dietary macronutrient content. The combination of measuring respiratory gas (O2 and CO2) exchange, nitrogenous (ammonia and urea) excretion, specifi...

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Autores principales:	Shuangyao Wang, Chris G. Carter, Quinn P. Fitzgibbon, Basseer M. Codabaccus, Gregory G. Smith
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11e0cc2650094c40836db10e7166ed91
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spelling	oai:doaj.org-article:11e0cc2650094c40836db10e7166ed912021-12-02T18:25:04ZEffect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi10.1038/s41598-021-91304-12045-2322https://doaj.org/article/11e0cc2650094c40836db10e7166ed912021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91304-1https://doaj.org/toc/2045-2322Abstract This is the first study in an aquatic ectotherm to combine a stoichiometric bioenergetic approach with an endpoint stochastic model to explore dietary macronutrient content. The combination of measuring respiratory gas (O2 and CO2) exchange, nitrogenous (ammonia and urea) excretion, specific dynamic action (SDA), metabolic energy substrate use, and whole-body protein synthesis in spiny lobster, Sagmariasus verreauxi, was examined in relation to dietary protein. Three isoenergetic feeds were formulated with varying crude protein: 40%, 50% and 60%, corresponding to CP40, CP50 and CP60 treatments, respectively. Total CO2 and ammonia excretion, SDA magnitude and coefficient, and protein synthesis in the CP60 treatment were higher compared to the CP40 treatment. These differences demonstrate dietary protein influences post-prandial energy metabolism. Metabolic use of each major energy substrate varied at different post-prandial times, indicating suitable amounts of high-quality protein with major non-protein energy-yielding nutrients, lipid and carbohydrate, are critical for lobsters. The average contribution of protein oxidation was lowest in the CP50 treatment, suggesting mechanisms underlying the most efficient retention of dietary protein and suitable dietary inclusion. This study advances understanding of how deficient and surplus dietary protein affects energy metabolism and provides approaches for fine-scale feed evaluation to support sustainable aquaculture.Shuangyao WangChris G. CarterQuinn P. FitzgibbonBasseer M. CodabaccusGregory G. SmithNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuangyao Wang Chris G. Carter Quinn P. Fitzgibbon Basseer M. Codabaccus Gregory G. Smith Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi
description	Abstract This is the first study in an aquatic ectotherm to combine a stoichiometric bioenergetic approach with an endpoint stochastic model to explore dietary macronutrient content. The combination of measuring respiratory gas (O2 and CO2) exchange, nitrogenous (ammonia and urea) excretion, specific dynamic action (SDA), metabolic energy substrate use, and whole-body protein synthesis in spiny lobster, Sagmariasus verreauxi, was examined in relation to dietary protein. Three isoenergetic feeds were formulated with varying crude protein: 40%, 50% and 60%, corresponding to CP40, CP50 and CP60 treatments, respectively. Total CO2 and ammonia excretion, SDA magnitude and coefficient, and protein synthesis in the CP60 treatment were higher compared to the CP40 treatment. These differences demonstrate dietary protein influences post-prandial energy metabolism. Metabolic use of each major energy substrate varied at different post-prandial times, indicating suitable amounts of high-quality protein with major non-protein energy-yielding nutrients, lipid and carbohydrate, are critical for lobsters. The average contribution of protein oxidation was lowest in the CP50 treatment, suggesting mechanisms underlying the most efficient retention of dietary protein and suitable dietary inclusion. This study advances understanding of how deficient and surplus dietary protein affects energy metabolism and provides approaches for fine-scale feed evaluation to support sustainable aquaculture.
format	article
author	Shuangyao Wang Chris G. Carter Quinn P. Fitzgibbon Basseer M. Codabaccus Gregory G. Smith
author_facet	Shuangyao Wang Chris G. Carter Quinn P. Fitzgibbon Basseer M. Codabaccus Gregory G. Smith
author_sort	Shuangyao Wang
title	Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi
title_short	Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi
title_full	Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi
title_fullStr	Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi
title_full_unstemmed	Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi
title_sort	effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster sagmariasus verreauxi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/11e0cc2650094c40836db10e7166ed91
work_keys_str_mv	AT shuangyaowang effectofdietaryproteinonenergymetabolismincludingproteinsynthesisinthespinylobstersagmariasusverreauxi AT chrisgcarter effectofdietaryproteinonenergymetabolismincludingproteinsynthesisinthespinylobstersagmariasusverreauxi AT quinnpfitzgibbon effectofdietaryproteinonenergymetabolismincludingproteinsynthesisinthespinylobstersagmariasusverreauxi AT basseermcodabaccus effectofdietaryproteinonenergymetabolismincludingproteinsynthesisinthespinylobstersagmariasusverreauxi AT gregorygsmith effectofdietaryproteinonenergymetabolismincludingproteinsynthesisinthespinylobstersagmariasusverreauxi
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Effect of dietary protein on energy metabolism including protein synthesis in the spiny lobster Sagmariasus verreauxi

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