Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis

The present study was targeted at investigating the effects of long intergenic non-protein coding RNA 1094 on breast cancer (BC) cell proliferation, apoptosis, and cell cycle and its related mechanism. In this study, Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were co...

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Autores principales:	Xia Wu, Cui Kong, Yilei Wu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	linc01094 mir-340-5p e2f3 bc proliferation apoptosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/11ed1514909844d9b705152960bd7222
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spelling	oai:doaj.org-article:11ed1514909844d9b705152960bd72222021-11-04T15:51:54ZLong intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis2165-59792165-598710.1080/21655979.2021.1993715https://doaj.org/article/11ed1514909844d9b705152960bd72222021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1993715https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987The present study was targeted at investigating the effects of long intergenic non-protein coding RNA 1094 on breast cancer (BC) cell proliferation, apoptosis, and cell cycle and its related mechanism. In this study, Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were conducted to detect the expressions of LINC01094, microRNA (miRNA, miR)-340-5p, and E2F transcription factor 3 (E2F3) in BC tissues and cells. With transfection, LINC01094 and miR-340-5p expressions were selectively up-regulated or down-regulated in BC cell lines, and then cell proliferation, cell cycle, and apoptosis were examined by cell counting kit-8 (CCK-8), 5-bromo-2ʹ-deoxyuridine (BrdU), and flow cytometry assays. Bioinformatics was utilized to predict the targeted relationships between miR-340-5p and LINC01094, as well as miR-340-5p and E2F3 mRNA 3ʹ-untranslated region (3ʹUTR), and RNA immunoprecipitation (RIP) assay and dual-luciferase reporter gene assay were employed to validate them. It was revealed that, LINC01094 expression was enhanced in BC cells and tissues, and LINC01094 overexpression promoted BC cell proliferation, accelerated cell cycle progression, and inhibited apoptosis while knocking down LINC01094 worked oppositely. LINC01094 directly targeted miR-340-5p and negatively regulated its expression in BC cells. Besides, E2F3 was substantiated to be the target gene of miR-340-5p, and E2F3 expression could be indirectly and positively modulated by LINC01094. All in all, LINC01094 promotes BC cell proliferation and cell cycle progression and inhibits apoptosis via modulating miR-340-5p/E2F3 molecular axis.Xia WuCui KongYilei WuTaylor & Francis Grouparticlelinc01094mir-340-5pe2f3bcproliferationapoptosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 9046-9057 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	linc01094 mir-340-5p e2f3 bc proliferation apoptosis Biotechnology TP248.13-248.65
spellingShingle	linc01094 mir-340-5p e2f3 bc proliferation apoptosis Biotechnology TP248.13-248.65 Xia Wu Cui Kong Yilei Wu Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis
description	The present study was targeted at investigating the effects of long intergenic non-protein coding RNA 1094 on breast cancer (BC) cell proliferation, apoptosis, and cell cycle and its related mechanism. In this study, Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were conducted to detect the expressions of LINC01094, microRNA (miRNA, miR)-340-5p, and E2F transcription factor 3 (E2F3) in BC tissues and cells. With transfection, LINC01094 and miR-340-5p expressions were selectively up-regulated or down-regulated in BC cell lines, and then cell proliferation, cell cycle, and apoptosis were examined by cell counting kit-8 (CCK-8), 5-bromo-2ʹ-deoxyuridine (BrdU), and flow cytometry assays. Bioinformatics was utilized to predict the targeted relationships between miR-340-5p and LINC01094, as well as miR-340-5p and E2F3 mRNA 3ʹ-untranslated region (3ʹUTR), and RNA immunoprecipitation (RIP) assay and dual-luciferase reporter gene assay were employed to validate them. It was revealed that, LINC01094 expression was enhanced in BC cells and tissues, and LINC01094 overexpression promoted BC cell proliferation, accelerated cell cycle progression, and inhibited apoptosis while knocking down LINC01094 worked oppositely. LINC01094 directly targeted miR-340-5p and negatively regulated its expression in BC cells. Besides, E2F3 was substantiated to be the target gene of miR-340-5p, and E2F3 expression could be indirectly and positively modulated by LINC01094. All in all, LINC01094 promotes BC cell proliferation and cell cycle progression and inhibits apoptosis via modulating miR-340-5p/E2F3 molecular axis.
format	article
author	Xia Wu Cui Kong Yilei Wu
author_facet	Xia Wu Cui Kong Yilei Wu
author_sort	Xia Wu
title	Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis
title_short	Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis
title_full	Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis
title_fullStr	Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis
title_full_unstemmed	Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis
title_sort	long intergenic non-protein coding rna 1094 (linc01094) promotes the progression of breast cancer (bc) by regulating the microrna-340-5p (mir-340-5p)/e2f transcription factor 3 (e2f3) axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/11ed1514909844d9b705152960bd7222
work_keys_str_mv	AT xiawu longintergenicnonproteincodingrna1094linc01094promotestheprogressionofbreastcancerbcbyregulatingthemicrorna3405pmir3405pe2ftranscriptionfactor3e2f3axis AT cuikong longintergenicnonproteincodingrna1094linc01094promotestheprogressionofbreastcancerbcbyregulatingthemicrorna3405pmir3405pe2ftranscriptionfactor3e2f3axis AT yileiwu longintergenicnonproteincodingrna1094linc01094promotestheprogressionofbreastcancerbcbyregulatingthemicrorna3405pmir3405pe2ftranscriptionfactor3e2f3axis
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Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis

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