Binding of plasma proteins to titanium dioxide nanotubes with different diameters

Mukta Kulkarni,1,* Ajda Fla&scaron;ker,1,* Maru&scaron;a Lokar,1 Katju&scaron;a Mrak-Polj&scaron;ak,2 Anca Mazare,3 Andrej Artenjak,4 Sa&scaron;a Čučnik,2 Slavko Kralj,5 Aljaž Velikonja,1 Patrik Schmuki,3 Veronika Kralj-Iglič,6 Snezna Sodin-Semrl,2,7 Ale&scaron; Iglič11Labor...

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Autores principales:	Kulkarni M, Fla&scaron;ker A, Lokar M, Mrak-Polj&scaron;ak K, Mazare A, Artenjak A, Čučnik S, Kralj S, Velikonja A, Schmuki P, Kralj-Iglič V, Sodin-Semrl S, Iglič A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/11f20cfa209945aab324a71b6882f4f1
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spelling	oai:doaj.org-article:11f20cfa209945aab324a71b6882f4f12021-12-02T00:54:48ZBinding of plasma proteins to titanium dioxide nanotubes with different diameters1178-2013https://doaj.org/article/11f20cfa209945aab324a71b6882f4f12015-02-01T00:00:00Zhttp://www.dovepress.com/binding-of-plasma-proteins-to-titanium-dioxide-nanotubes-with-differen-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Mukta Kulkarni,1,* Ajda Fla&scaron;ker,1,* Maru&scaron;a Lokar,1 Katju&scaron;a Mrak-Polj&scaron;ak,2 Anca Mazare,3 Andrej Artenjak,4 Sa&scaron;a Čučnik,2 Slavko Kralj,5 Aljaž Velikonja,1 Patrik Schmuki,3 Veronika Kralj-Iglič,6 Snezna Sodin-Semrl,2,7 Ale&scaron; Iglič11Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia; 2Department of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia; 3Department of Materials Science and Engineering, University of Erlangen Nuremberg, Erlangen, Germany; 4Sandoz Biopharmaceuticals Menge&scaron;, Lek Pharmaceuticals dd, Menges, Slovenia; 5Department for Materials Synthesis, Institute Jožef Stefan (IJS), Ljubljana, Slovenia; 6Faculty of Health Studies, University of Ljubljana, Ljubljana, Slovenia; 7Faculty of Mathematics, Natural Science and Information Technology, University of Primorska, Koper, Slovenia *These authors contributed equally to this workAbstract: Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO2) nanotubes (NTs) by electrochemical anodization. The zeta potential (ζ-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of ζ-potential on salt concentration, whereby the absolute value of ζ-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considering the NT lengths) is available for 100 nm NTs, with decreasing surface area for 50 and 15 nm NTs. These current investigations will have an impact on increasing the binding ability of biomedical devices in the body leading to increased durability of biomedical devices. Keywords: protein binding, serum amyloid A, β2-glycoprotein I, immunoglobulin G, histone IIAKulkarni MFla&scaron;ker ALokar MMrak-Polj&scaron;ak KMazare AArtenjak AČučnik SKralj SVelikonja ASchmuki PKralj-Iglič VSodin-Semrl SIglič ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1359-1373 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kulkarni M Fla&scaron;ker A Lokar M Mrak-Polj&scaron;ak K Mazare A Artenjak A Čučnik S Kralj S Velikonja A Schmuki P Kralj-Iglič V Sodin-Semrl S Iglič A Binding of plasma proteins to titanium dioxide nanotubes with different diameters
description	Mukta Kulkarni,1,* Ajda Fla&scaron;ker,1,* Maru&scaron;a Lokar,1 Katju&scaron;a Mrak-Polj&scaron;ak,2 Anca Mazare,3 Andrej Artenjak,4 Sa&scaron;a Čučnik,2 Slavko Kralj,5 Aljaž Velikonja,1 Patrik Schmuki,3 Veronika Kralj-Iglič,6 Snezna Sodin-Semrl,2,7 Ale&scaron; Iglič11Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia; 2Department of Rheumatology, University Medical Centre Ljubljana, Ljubljana, Slovenia; 3Department of Materials Science and Engineering, University of Erlangen Nuremberg, Erlangen, Germany; 4Sandoz Biopharmaceuticals Menge&scaron;, Lek Pharmaceuticals dd, Menges, Slovenia; 5Department for Materials Synthesis, Institute Jožef Stefan (IJS), Ljubljana, Slovenia; 6Faculty of Health Studies, University of Ljubljana, Ljubljana, Slovenia; 7Faculty of Mathematics, Natural Science and Information Technology, University of Primorska, Koper, Slovenia *These authors contributed equally to this workAbstract: Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO2) nanotubes (NTs) by electrochemical anodization. The zeta potential (ζ-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of ζ-potential on salt concentration, whereby the absolute value of ζ-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considering the NT lengths) is available for 100 nm NTs, with decreasing surface area for 50 and 15 nm NTs. These current investigations will have an impact on increasing the binding ability of biomedical devices in the body leading to increased durability of biomedical devices. Keywords: protein binding, serum amyloid A, β2-glycoprotein I, immunoglobulin G, histone IIA
format	article
author	Kulkarni M Fla&scaron;ker A Lokar M Mrak-Polj&scaron;ak K Mazare A Artenjak A Čučnik S Kralj S Velikonja A Schmuki P Kralj-Iglič V Sodin-Semrl S Iglič A
author_facet	Kulkarni M Fla&scaron;ker A Lokar M Mrak-Polj&scaron;ak K Mazare A Artenjak A Čučnik S Kralj S Velikonja A Schmuki P Kralj-Iglič V Sodin-Semrl S Iglič A
author_sort	Kulkarni M
title	Binding of plasma proteins to titanium dioxide nanotubes with different diameters
title_short	Binding of plasma proteins to titanium dioxide nanotubes with different diameters
title_full	Binding of plasma proteins to titanium dioxide nanotubes with different diameters
title_fullStr	Binding of plasma proteins to titanium dioxide nanotubes with different diameters
title_full_unstemmed	Binding of plasma proteins to titanium dioxide nanotubes with different diameters
title_sort	binding of plasma proteins to titanium dioxide nanotubes with different diameters
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/11f20cfa209945aab324a71b6882f4f1
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Binding of plasma proteins to titanium dioxide nanotubes with different diameters

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