An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages

Abstract Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-bind...

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Autores principales:	Hye-Ran Kim, Hyun-Su Lee, Kyung-Sik Lee, In Duk Jung, Min-Sung Kwon, Chang-Hyun Kim, Seong-Min Kim, Myung-Han Yoon, Yeong-Min Park, Sang-Myeong Lee, Chang-Duk Jun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11f45bf08b16472495e0a1ada3918f2d
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spelling	oai:doaj.org-article:11f45bf08b16472495e0a1ada3918f2d2021-12-02T15:06:00ZAn Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages10.1038/s41598-017-09144-x2045-2322https://doaj.org/article/11f45bf08b16472495e0a1ada3918f2d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09144-xhttps://doaj.org/toc/2045-2322Abstract Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-binding protein, in Toll-like receptor (TLR)-stimulated phagocytosis. TAGLN2 was greatly induced in macrophages in response to lipopolysaccharide (LPS), a ligand for TLR4, partly via the NF-κB pathway. TAGLN2-deficient macrophages (TAGLN2 −/−) showed defective phagocytic functions of IgM- and IgG-coated sheep red blood cells as well as bacteria. Cell signaling pathways involved in actin rearrangement—PI3 kinase/AKT and Ras-ERK—were also down-regulated in LPS-stimulated TAGLN2-deficient macrophages. Moreover, TAGLN2 −/− mice showed higher mortality after bacterial infection than wild-type littermates. Thus, our results revealed a novel function of TAGLN2 as a molecular armament required for host defense.Hye-Ran KimHyun-Su LeeKyung-Sik LeeIn Duk JungMin-Sung KwonChang-Hyun KimSeong-Min KimMyung-Han YoonYeong-Min ParkSang-Myeong LeeChang-Duk JunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hye-Ran Kim Hyun-Su Lee Kyung-Sik Lee In Duk Jung Min-Sung Kwon Chang-Hyun Kim Seong-Min Kim Myung-Han Yoon Yeong-Min Park Sang-Myeong Lee Chang-Duk Jun An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
description	Abstract Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-binding protein, in Toll-like receptor (TLR)-stimulated phagocytosis. TAGLN2 was greatly induced in macrophages in response to lipopolysaccharide (LPS), a ligand for TLR4, partly via the NF-κB pathway. TAGLN2-deficient macrophages (TAGLN2 −/−) showed defective phagocytic functions of IgM- and IgG-coated sheep red blood cells as well as bacteria. Cell signaling pathways involved in actin rearrangement—PI3 kinase/AKT and Ras-ERK—were also down-regulated in LPS-stimulated TAGLN2-deficient macrophages. Moreover, TAGLN2 −/− mice showed higher mortality after bacterial infection than wild-type littermates. Thus, our results revealed a novel function of TAGLN2 as a molecular armament required for host defense.
format	article
author	Hye-Ran Kim Hyun-Su Lee Kyung-Sik Lee In Duk Jung Min-Sung Kwon Chang-Hyun Kim Seong-Min Kim Myung-Han Yoon Yeong-Min Park Sang-Myeong Lee Chang-Duk Jun
author_facet	Hye-Ran Kim Hyun-Su Lee Kyung-Sik Lee In Duk Jung Min-Sung Kwon Chang-Hyun Kim Seong-Min Kim Myung-Han Yoon Yeong-Min Park Sang-Myeong Lee Chang-Duk Jun
author_sort	Hye-Ran Kim
title	An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
title_short	An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
title_full	An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
title_fullStr	An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
title_full_unstemmed	An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages
title_sort	essential role for tagln2 in phagocytosis of lipopolysaccharide-activated macrophages
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/11f45bf08b16472495e0a1ada3918f2d
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An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages

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