The possible molecular mechanisms of bisphenol A action on porcine early embryonic development

Abstract Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. However, the mechanism of action of BPA is limited. In this study, we investigated...

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Autores principales:	Jing Guo, Ming-Hui Zhao, Kyung-Tae Shin, Ying-Jie Niu, Yong-Dae Ahn, Nam-Hyung Kim, Xiang-Shun Cui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11fc035417ff43789b484edaa6dceee9
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spelling	oai:doaj.org-article:11fc035417ff43789b484edaa6dceee92021-12-02T16:06:02ZThe possible molecular mechanisms of bisphenol A action on porcine early embryonic development10.1038/s41598-017-09282-22045-2322https://doaj.org/article/11fc035417ff43789b484edaa6dceee92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09282-2https://doaj.org/toc/2045-2322Abstract Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. However, the mechanism of action of BPA is limited. In this study, we investigated the role and mechanism of BPA in porcine embryonic development. First, the parthenotes were treated with different concentrations of BPA. We found that blastocyst formation was impaired and the parthenotes were arrested at the 4-cell stage after treatment with 100 μm BPA. Second, ROS increased following the addition of BPA, which further caused mitochondrial damage, and cytochrome c was released from the mitochondria to induce apoptosis. The adaptive response was demonstrated through LC3 immunofluorescence staining and by assessing autophagy-related gene expression. In addition, BPA caused DNA damage through the p53-p21 signaling pathway. Thus, our results indicate that BPA displays an adverse effect on porcine early embryonic development through mitochondrial and DNA damage.Jing GuoMing-Hui ZhaoKyung-Tae ShinYing-Jie NiuYong-Dae AhnNam-Hyung KimXiang-Shun CuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Guo Ming-Hui Zhao Kyung-Tae Shin Ying-Jie Niu Yong-Dae Ahn Nam-Hyung Kim Xiang-Shun Cui The possible molecular mechanisms of bisphenol A action on porcine early embryonic development
description	Abstract Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. However, the mechanism of action of BPA is limited. In this study, we investigated the role and mechanism of BPA in porcine embryonic development. First, the parthenotes were treated with different concentrations of BPA. We found that blastocyst formation was impaired and the parthenotes were arrested at the 4-cell stage after treatment with 100 μm BPA. Second, ROS increased following the addition of BPA, which further caused mitochondrial damage, and cytochrome c was released from the mitochondria to induce apoptosis. The adaptive response was demonstrated through LC3 immunofluorescence staining and by assessing autophagy-related gene expression. In addition, BPA caused DNA damage through the p53-p21 signaling pathway. Thus, our results indicate that BPA displays an adverse effect on porcine early embryonic development through mitochondrial and DNA damage.
format	article
author	Jing Guo Ming-Hui Zhao Kyung-Tae Shin Ying-Jie Niu Yong-Dae Ahn Nam-Hyung Kim Xiang-Shun Cui
author_facet	Jing Guo Ming-Hui Zhao Kyung-Tae Shin Ying-Jie Niu Yong-Dae Ahn Nam-Hyung Kim Xiang-Shun Cui
author_sort	Jing Guo
title	The possible molecular mechanisms of bisphenol A action on porcine early embryonic development
title_short	The possible molecular mechanisms of bisphenol A action on porcine early embryonic development
title_full	The possible molecular mechanisms of bisphenol A action on porcine early embryonic development
title_fullStr	The possible molecular mechanisms of bisphenol A action on porcine early embryonic development
title_full_unstemmed	The possible molecular mechanisms of bisphenol A action on porcine early embryonic development
title_sort	possible molecular mechanisms of bisphenol a action on porcine early embryonic development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/11fc035417ff43789b484edaa6dceee9
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The possible molecular mechanisms of bisphenol A action on porcine early embryonic development

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