Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway

Seon Young Choi,1 Min Seok Song,1 Pan Dong Ryu,1 Anh Thu Ngoc Lam,2 Sang-Woo Joo,2 So Yeong Lee1 1Laboratory of Veterinary Pharmacology, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 2Department of Chemistry, Soongsil University, Seoul, South&...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Choi SY, Song MS, Ryu PD, Lam ATN, Joo SW, Lee SY
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/12050a5ee74d48f7bffb21372518d4c9
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:12050a5ee74d48f7bffb21372518d4c9
record_format dspace
spelling oai:doaj.org-article:12050a5ee74d48f7bffb21372518d4c92021-12-02T02:31:36ZGold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway1178-2013https://doaj.org/article/12050a5ee74d48f7bffb21372518d4c92015-07-01T00:00:00Zhttp://www.dovepress.com/gold-nanoparticles-promote-osteogenic-differentiation-in-human-adipose-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Seon Young Choi,1 Min Seok Song,1 Pan Dong Ryu,1 Anh Thu Ngoc Lam,2 Sang-Woo Joo,2 So Yeong Lee1 1Laboratory of Veterinary Pharmacology, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 2Department of Chemistry, Soongsil University, Seoul, South Korea Abstract: Gold nanoparticles (AuNPs) are attractive materials for use in biomedicine due to their physical properties. Increasing evidence suggests that several nanoparticles induce the differentiation of human mesenchymal stem cells into osteoblasts and adipocytes. In this study, we hypothesized that chitosan-conjugated AuNPs promote the osteogenic differentiation of human adipose-derived mesenchymal stem cells. For the evaluation of osteogenic differentiation, alizarin red staining, an alamarBlue® assay, and a quantitative real-time polymerase chain reaction analysis were performed. In order to examine specific signaling pathways, immunofluorescence and a western blotting assay were performed. Our results demonstrate that chitosan-conjugated AuNPs increase the deposition of calcium content and the expression of marker genes related to osteogenic differentiation in human adipose-derived mesenchymal stem cells at nontoxic concentrations. These results indicate that chitosan-conjugated AuNPs promote osteogenesis through the Wnt/β-catenin signaling pathway. Therefore, chitosan-conjugated AuNPs can be used as a reagent for promoting bone formation. Keywords: chitosan-conjugated gold nanoparticle, mineralization, nonphosphorylated beta-cateninChoi SYSong MSRyu PDLam ATNJoo SWLee SYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 4383-4392 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Choi SY
Song MS
Ryu PD
Lam ATN
Joo SW
Lee SY
Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
description Seon Young Choi,1 Min Seok Song,1 Pan Dong Ryu,1 Anh Thu Ngoc Lam,2 Sang-Woo Joo,2 So Yeong Lee1 1Laboratory of Veterinary Pharmacology, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 2Department of Chemistry, Soongsil University, Seoul, South Korea Abstract: Gold nanoparticles (AuNPs) are attractive materials for use in biomedicine due to their physical properties. Increasing evidence suggests that several nanoparticles induce the differentiation of human mesenchymal stem cells into osteoblasts and adipocytes. In this study, we hypothesized that chitosan-conjugated AuNPs promote the osteogenic differentiation of human adipose-derived mesenchymal stem cells. For the evaluation of osteogenic differentiation, alizarin red staining, an alamarBlue® assay, and a quantitative real-time polymerase chain reaction analysis were performed. In order to examine specific signaling pathways, immunofluorescence and a western blotting assay were performed. Our results demonstrate that chitosan-conjugated AuNPs increase the deposition of calcium content and the expression of marker genes related to osteogenic differentiation in human adipose-derived mesenchymal stem cells at nontoxic concentrations. These results indicate that chitosan-conjugated AuNPs promote osteogenesis through the Wnt/β-catenin signaling pathway. Therefore, chitosan-conjugated AuNPs can be used as a reagent for promoting bone formation. Keywords: chitosan-conjugated gold nanoparticle, mineralization, nonphosphorylated beta-catenin
format article
author Choi SY
Song MS
Ryu PD
Lam ATN
Joo SW
Lee SY
author_facet Choi SY
Song MS
Ryu PD
Lam ATN
Joo SW
Lee SY
author_sort Choi SY
title Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
title_short Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
title_full Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
title_fullStr Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
title_full_unstemmed Gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the Wnt/β-catenin signaling pathway
title_sort gold nanoparticles promote osteogenic differentiation in human adipose-derived mesenchymal stem cells through the wnt/β-catenin signaling pathway
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/12050a5ee74d48f7bffb21372518d4c9
work_keys_str_mv AT choisy goldnanoparticlespromoteosteogenicdifferentiationinhumanadiposederivedmesenchymalstemcellsthroughthewntbetacateninsignalingpathway
AT songms goldnanoparticlespromoteosteogenicdifferentiationinhumanadiposederivedmesenchymalstemcellsthroughthewntbetacateninsignalingpathway
AT ryupd goldnanoparticlespromoteosteogenicdifferentiationinhumanadiposederivedmesenchymalstemcellsthroughthewntbetacateninsignalingpathway
AT lamatn goldnanoparticlespromoteosteogenicdifferentiationinhumanadiposederivedmesenchymalstemcellsthroughthewntbetacateninsignalingpathway
AT joosw goldnanoparticlespromoteosteogenicdifferentiationinhumanadiposederivedmesenchymalstemcellsthroughthewntbetacateninsignalingpathway
AT leesy goldnanoparticlespromoteosteogenicdifferentiationinhumanadiposederivedmesenchymalstemcellsthroughthewntbetacateninsignalingpathway
_version_ 1718402391044259840

Ejemplares similares