ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells

RCE-4, a steroidal saponin isolated from <i>Reineckia carnea</i>, has been studied previously and has exhibited promising anti-cervical cancer properties by inducing programmed cell death (PCD) of Ca Ski cells. Considering the cancer cells developed various pathways to evade chemotherapy...

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Autores principales:	Fang-Fang You, Jing Zhang, Fan Cheng, Kun Zou, Xue-Qing Zhang, Jian-Feng Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	RCE-4 PCD ATG 4B the Bcl-2–Beclin 1 complex Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1205eb8d0b97422b8433f0f4c290311e
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spelling	oai:doaj.org-article:1205eb8d0b97422b8433f0f4c290311e2021-11-25T17:55:14ZATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells10.3390/ijms2222123021422-00671661-6596https://doaj.org/article/1205eb8d0b97422b8433f0f4c290311e2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12302https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067RCE-4, a steroidal saponin isolated from <i>Reineckia carnea</i>, has been studied previously and has exhibited promising anti-cervical cancer properties by inducing programmed cell death (PCD) of Ca Ski cells. Considering the cancer cells developed various pathways to evade chemotherapy-induced PCD, there is, therefore, an urgent need to further explore the potential mechanisms underlying its actions. The present study focused on targeting the Bcl-2–Beclin 1 complex, which is known as the key regulator of PCD, to deeply elucidate the molecular mechanism of RCE-4 against cervical cancer. The effects of RCE-4 on the Bcl-2–Beclin 1 complex were investigated by using the co-immunoprecipitation assay. In addition, autophagy-related genes (ATG) were also analyzed due to their special roles in PCD. The results demonstrated that RCE-4 inhibited the formation of the Bcl-2–Beclin 1 complex in Ca Ski cells via various pathways, and ATG 4B proteins involved in this process served as a key co-factor. Furthermore, based on the above, the sensitivity of RCE-4 to Ca Ski cells was significantly enhanced by inhibiting the expression of the ATG 4B by applying the ATG 4B siRNA plasmid.Fang-Fang YouJing ZhangFan ChengKun ZouXue-Qing ZhangJian-Feng ChenMDPI AGarticleRCE-4PCDATG 4Bthe Bcl-2–Beclin 1 complexBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12302, p 12302 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RCE-4 PCD ATG 4B the Bcl-2–Beclin 1 complex Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	RCE-4 PCD ATG 4B the Bcl-2–Beclin 1 complex Biology (General) QH301-705.5 Chemistry QD1-999 Fang-Fang You Jing Zhang Fan Cheng Kun Zou Xue-Qing Zhang Jian-Feng Chen ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
description	RCE-4, a steroidal saponin isolated from <i>Reineckia carnea</i>, has been studied previously and has exhibited promising anti-cervical cancer properties by inducing programmed cell death (PCD) of Ca Ski cells. Considering the cancer cells developed various pathways to evade chemotherapy-induced PCD, there is, therefore, an urgent need to further explore the potential mechanisms underlying its actions. The present study focused on targeting the Bcl-2–Beclin 1 complex, which is known as the key regulator of PCD, to deeply elucidate the molecular mechanism of RCE-4 against cervical cancer. The effects of RCE-4 on the Bcl-2–Beclin 1 complex were investigated by using the co-immunoprecipitation assay. In addition, autophagy-related genes (ATG) were also analyzed due to their special roles in PCD. The results demonstrated that RCE-4 inhibited the formation of the Bcl-2–Beclin 1 complex in Ca Ski cells via various pathways, and ATG 4B proteins involved in this process served as a key co-factor. Furthermore, based on the above, the sensitivity of RCE-4 to Ca Ski cells was significantly enhanced by inhibiting the expression of the ATG 4B by applying the ATG 4B siRNA plasmid.
format	article
author	Fang-Fang You Jing Zhang Fan Cheng Kun Zou Xue-Qing Zhang Jian-Feng Chen
author_facet	Fang-Fang You Jing Zhang Fan Cheng Kun Zou Xue-Qing Zhang Jian-Feng Chen
author_sort	Fang-Fang You
title	ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
title_short	ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
title_full	ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
title_fullStr	ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
title_full_unstemmed	ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells
title_sort	atg 4b serves a crucial role in rce-4-induced inhibition of the bcl-2–beclin 1 complex in cervical cancer ca ski cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1205eb8d0b97422b8433f0f4c290311e
work_keys_str_mv	AT fangfangyou atg4bservesacrucialroleinrce4inducedinhibitionofthebcl2beclin1complexincervicalcancercaskicells AT jingzhang atg4bservesacrucialroleinrce4inducedinhibitionofthebcl2beclin1complexincervicalcancercaskicells AT fancheng atg4bservesacrucialroleinrce4inducedinhibitionofthebcl2beclin1complexincervicalcancercaskicells AT kunzou atg4bservesacrucialroleinrce4inducedinhibitionofthebcl2beclin1complexincervicalcancercaskicells AT xueqingzhang atg4bservesacrucialroleinrce4inducedinhibitionofthebcl2beclin1complexincervicalcancercaskicells AT jianfengchen atg4bservesacrucialroleinrce4inducedinhibitionofthebcl2beclin1complexincervicalcancercaskicells
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ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2–Beclin 1 Complex in Cervical Cancer Ca Ski Cells

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