A role for SPARC in the moderation of human insulin secretion.

<h4>Aims/hypothesis</h4>We have previously shown the implication of the multifunctional protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in insulin resistance but potential effects on beta-cell function have not been assessed. We therefore aimed to characterise th...

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Autores principales:	Lorna W Harries, Laura J McCulloch, Janet E Holley, Thomas J Rawling, Hannah J Welters, Katarina Kos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/120dab7d1f0b4acba4c15e4675fe6096
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spelling	oai:doaj.org-article:120dab7d1f0b4acba4c15e4675fe60962021-11-18T07:39:19ZA role for SPARC in the moderation of human insulin secretion.1932-620310.1371/journal.pone.0068253https://doaj.org/article/120dab7d1f0b4acba4c15e4675fe60962013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840838/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aims/hypothesis</h4>We have previously shown the implication of the multifunctional protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in insulin resistance but potential effects on beta-cell function have not been assessed. We therefore aimed to characterise the effect of SPARC on beta-cell function and features of diabetes.<h4>Methods</h4>We measured SPARC expression by qRT-PCR in human primary pancreatic islets, adipose tissue, liver and muscle. We then examined the relation of SPARC with glucose stimulated insulin secretion (GSIS) in primary human islets and the effect of SPARC overexpression on GSIS in beta cell lines.<h4>Results</h4>SPARC was expressed at measurable levels in human islets, adipose tissue, liver and skeletal muscle, and demonstrated reduced expression in primary islets from subjects with diabetes compared with controls (p< = 0.05). SPARC levels were positively correlated with GSIS in islets from control donors (p< = 0.01). Overexpression of SPARC in cultured beta-cells resulted in a 2.4-fold increase in insulin secretion in high glucose conditions (p< = 0.01).<h4>Conclusions</h4>Our data suggest that levels of SPARC are reduced in islets from donors with diabetes and that it has a role in insulin secretion, an effect which appears independent of SPARC's modulation of obesity-induced insulin resistance in adipose tissue.Lorna W HarriesLaura J McCullochJanet E HolleyThomas J RawlingHannah J WeltersKatarina KosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e68253 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lorna W Harries Laura J McCulloch Janet E Holley Thomas J Rawling Hannah J Welters Katarina Kos A role for SPARC in the moderation of human insulin secretion.
description	<h4>Aims/hypothesis</h4>We have previously shown the implication of the multifunctional protein SPARC (Secreted protein acidic and rich in cysteine)/osteonectin in insulin resistance but potential effects on beta-cell function have not been assessed. We therefore aimed to characterise the effect of SPARC on beta-cell function and features of diabetes.<h4>Methods</h4>We measured SPARC expression by qRT-PCR in human primary pancreatic islets, adipose tissue, liver and muscle. We then examined the relation of SPARC with glucose stimulated insulin secretion (GSIS) in primary human islets and the effect of SPARC overexpression on GSIS in beta cell lines.<h4>Results</h4>SPARC was expressed at measurable levels in human islets, adipose tissue, liver and skeletal muscle, and demonstrated reduced expression in primary islets from subjects with diabetes compared with controls (p< = 0.05). SPARC levels were positively correlated with GSIS in islets from control donors (p< = 0.01). Overexpression of SPARC in cultured beta-cells resulted in a 2.4-fold increase in insulin secretion in high glucose conditions (p< = 0.01).<h4>Conclusions</h4>Our data suggest that levels of SPARC are reduced in islets from donors with diabetes and that it has a role in insulin secretion, an effect which appears independent of SPARC's modulation of obesity-induced insulin resistance in adipose tissue.
format	article
author	Lorna W Harries Laura J McCulloch Janet E Holley Thomas J Rawling Hannah J Welters Katarina Kos
author_facet	Lorna W Harries Laura J McCulloch Janet E Holley Thomas J Rawling Hannah J Welters Katarina Kos
author_sort	Lorna W Harries
title	A role for SPARC in the moderation of human insulin secretion.
title_short	A role for SPARC in the moderation of human insulin secretion.
title_full	A role for SPARC in the moderation of human insulin secretion.
title_fullStr	A role for SPARC in the moderation of human insulin secretion.
title_full_unstemmed	A role for SPARC in the moderation of human insulin secretion.
title_sort	role for sparc in the moderation of human insulin secretion.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/120dab7d1f0b4acba4c15e4675fe6096
work_keys_str_mv	AT lornawharries aroleforsparcinthemoderationofhumaninsulinsecretion AT laurajmcculloch aroleforsparcinthemoderationofhumaninsulinsecretion AT janeteholley aroleforsparcinthemoderationofhumaninsulinsecretion AT thomasjrawling aroleforsparcinthemoderationofhumaninsulinsecretion AT hannahjwelters aroleforsparcinthemoderationofhumaninsulinsecretion AT katarinakos aroleforsparcinthemoderationofhumaninsulinsecretion AT lornawharries roleforsparcinthemoderationofhumaninsulinsecretion AT laurajmcculloch roleforsparcinthemoderationofhumaninsulinsecretion AT janeteholley roleforsparcinthemoderationofhumaninsulinsecretion AT thomasjrawling roleforsparcinthemoderationofhumaninsulinsecretion AT hannahjwelters roleforsparcinthemoderationofhumaninsulinsecretion AT katarinakos roleforsparcinthemoderationofhumaninsulinsecretion
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A role for SPARC in the moderation of human insulin secretion.

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