Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals

Abstract The aim of the present study is to describe the ongoing spread of the KPC-producing strains, which is evolving to an epidemic in Czech hospitals. During the period of 2018–2019, a total of 108 KPC-producing Enterobacterales were recovered from 20 hospitals. Analysis of long-read sequencing...

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Autores principales:	Lucie Kraftova, Marc Finianos, Vendula Studentova, Katerina Chudejova, Vladislav Jakubu, Helena Zemlickova, Costas C. Papagiannitsis, Ibrahim Bitar, Jaroslav Hrabak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12104598a92a478f8c6e2efc4d47ef4c
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spelling	oai:doaj.org-article:12104598a92a478f8c6e2efc4d47ef4c2021-12-02T16:35:42ZEvidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals10.1038/s41598-021-95285-z2045-2322https://doaj.org/article/12104598a92a478f8c6e2efc4d47ef4c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95285-zhttps://doaj.org/toc/2045-2322Abstract The aim of the present study is to describe the ongoing spread of the KPC-producing strains, which is evolving to an epidemic in Czech hospitals. During the period of 2018–2019, a total of 108 KPC-producing Enterobacterales were recovered from 20 hospitals. Analysis of long-read sequencing data revealed the presence of several types of bla KPC-carrying plasmids; 19 out of 25 bla KPC-carrying plasmids could be assigned to R (n = 12), N (n = 5), C (n = 1) and P6 (n = 1) incompatibility (Inc) groups. Five of the remaining bla KPC-carrying plasmids were multireplicon, while one plasmid couldn’t be typed. Additionally, phylogenetic analysis confirmed the spread of bla KPC-carrying plasmids among different clones of diverse Enterobacterales species. Our findings demonstrated that the increased prevalence of KPC-producing isolates was due to plasmids spreading among different species. In some districts, the local dissemination of IncR and IncN plasmids was observed. Additionally, the ongoing evolution of bla KPC-carrying plasmids, through genetic rearrangements, favours the preservation and further dissemination of these mobile genetic elements. Therefore, the situation should be monitored, and immediate infection control should be implemented in hospitals reporting KPC-producing strains.Lucie KraftovaMarc FinianosVendula StudentovaKaterina ChudejovaVladislav JakubuHelena ZemlickovaCostas C. PapagiannitsisIbrahim BitarJaroslav HrabakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucie Kraftova Marc Finianos Vendula Studentova Katerina Chudejova Vladislav Jakubu Helena Zemlickova Costas C. Papagiannitsis Ibrahim Bitar Jaroslav Hrabak Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
description	Abstract The aim of the present study is to describe the ongoing spread of the KPC-producing strains, which is evolving to an epidemic in Czech hospitals. During the period of 2018–2019, a total of 108 KPC-producing Enterobacterales were recovered from 20 hospitals. Analysis of long-read sequencing data revealed the presence of several types of bla KPC-carrying plasmids; 19 out of 25 bla KPC-carrying plasmids could be assigned to R (n = 12), N (n = 5), C (n = 1) and P6 (n = 1) incompatibility (Inc) groups. Five of the remaining bla KPC-carrying plasmids were multireplicon, while one plasmid couldn’t be typed. Additionally, phylogenetic analysis confirmed the spread of bla KPC-carrying plasmids among different clones of diverse Enterobacterales species. Our findings demonstrated that the increased prevalence of KPC-producing isolates was due to plasmids spreading among different species. In some districts, the local dissemination of IncR and IncN plasmids was observed. Additionally, the ongoing evolution of bla KPC-carrying plasmids, through genetic rearrangements, favours the preservation and further dissemination of these mobile genetic elements. Therefore, the situation should be monitored, and immediate infection control should be implemented in hospitals reporting KPC-producing strains.
format	article
author	Lucie Kraftova Marc Finianos Vendula Studentova Katerina Chudejova Vladislav Jakubu Helena Zemlickova Costas C. Papagiannitsis Ibrahim Bitar Jaroslav Hrabak
author_facet	Lucie Kraftova Marc Finianos Vendula Studentova Katerina Chudejova Vladislav Jakubu Helena Zemlickova Costas C. Papagiannitsis Ibrahim Bitar Jaroslav Hrabak
author_sort	Lucie Kraftova
title	Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
title_short	Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
title_full	Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
title_fullStr	Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
title_full_unstemmed	Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals
title_sort	evidence of an epidemic spread of kpc-producing enterobacterales in czech hospitals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/12104598a92a478f8c6e2efc4d47ef4c
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Evidence of an epidemic spread of KPC-producing Enterobacterales in Czech hospitals

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