Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila

Abstract The immune system of sturgeons, one of the most ancient and economically valuable fish worldwide, is poorly understood. The lack of molecular tools and data about infection biomarkers hinders the possibility to monitor sturgeon health during farming and detect infection outbreaks. To tackle...

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Autores principales:	Mauricio Castellano, Valeria Silva-Álvarez, Marcio Aversa-Marnai, María Lamas-Bervejillo, Ignacio Quartiani, Alejandro Perretta, Andrea Villarino, Ana María Ferreira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1210cd24feae4cd1882d11a5f7f294ee
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spelling	oai:doaj.org-article:1210cd24feae4cd1882d11a5f7f294ee2021-12-02T12:42:17ZSerum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila10.1038/s41598-020-79065-92045-2322https://doaj.org/article/1210cd24feae4cd1882d11a5f7f294ee2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79065-9https://doaj.org/toc/2045-2322Abstract The immune system of sturgeons, one of the most ancient and economically valuable fish worldwide, is poorly understood. The lack of molecular tools and data about infection biomarkers hinders the possibility to monitor sturgeon health during farming and detect infection outbreaks. To tackle this issue, we mined publicly available transcriptomic datasets and identified putative positive acute-phase proteins (APPs) of Russian sturgeons that could be induced by a bacterial infection and monitored using non-invasive methods. Teleost literature compelled us to focus on five promising candidates: hepcidin, a warm acclimation associated hemopexin, intelectin, serum amyloid A protein (SAA) and serotransferrin. Among them, SAA was the most upregulated protein at the mRNA level in the liver of sturgeons challenged with heat-inactivated or live Aeromonas hydrophila. To assess whether this upregulation yielded increasing SAA levels in circulation, we developed an in-house ELISA to quantify SAA levels in sturgeon serum. Circulating SAA rose upon bacterial challenge and positively correlated with hepatic saa expression. This is the first time serum SAA has been quantified in an Actinopterygii fish. Since APPs vary across different fish species, our work sheds light on sturgeon acute-phase response, revealing that SAA is a positive APP with potential value as infection biomarker.Mauricio CastellanoValeria Silva-ÁlvarezMarcio Aversa-MarnaiMaría Lamas-BervejilloIgnacio QuartianiAlejandro PerrettaAndrea VillarinoAna María FerreiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mauricio Castellano Valeria Silva-Álvarez Marcio Aversa-Marnai María Lamas-Bervejillo Ignacio Quartiani Alejandro Perretta Andrea Villarino Ana María Ferreira Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila
description	Abstract The immune system of sturgeons, one of the most ancient and economically valuable fish worldwide, is poorly understood. The lack of molecular tools and data about infection biomarkers hinders the possibility to monitor sturgeon health during farming and detect infection outbreaks. To tackle this issue, we mined publicly available transcriptomic datasets and identified putative positive acute-phase proteins (APPs) of Russian sturgeons that could be induced by a bacterial infection and monitored using non-invasive methods. Teleost literature compelled us to focus on five promising candidates: hepcidin, a warm acclimation associated hemopexin, intelectin, serum amyloid A protein (SAA) and serotransferrin. Among them, SAA was the most upregulated protein at the mRNA level in the liver of sturgeons challenged with heat-inactivated or live Aeromonas hydrophila. To assess whether this upregulation yielded increasing SAA levels in circulation, we developed an in-house ELISA to quantify SAA levels in sturgeon serum. Circulating SAA rose upon bacterial challenge and positively correlated with hepatic saa expression. This is the first time serum SAA has been quantified in an Actinopterygii fish. Since APPs vary across different fish species, our work sheds light on sturgeon acute-phase response, revealing that SAA is a positive APP with potential value as infection biomarker.
format	article
author	Mauricio Castellano Valeria Silva-Álvarez Marcio Aversa-Marnai María Lamas-Bervejillo Ignacio Quartiani Alejandro Perretta Andrea Villarino Ana María Ferreira
author_facet	Mauricio Castellano Valeria Silva-Álvarez Marcio Aversa-Marnai María Lamas-Bervejillo Ignacio Quartiani Alejandro Perretta Andrea Villarino Ana María Ferreira
author_sort	Mauricio Castellano
title	Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila
title_short	Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila
title_full	Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila
title_fullStr	Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila
title_full_unstemmed	Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila
title_sort	serum amyloid a is a positive acute phase protein in russian sturgeon challenged with aeromonas hydrophila
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/1210cd24feae4cd1882d11a5f7f294ee
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Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila

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