Lactic Acid Bacteria Fermented Cordyceps militaris (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model

Cordyceps militaris (C. militaris) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharide...

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Autores principales:	Abdul-Rehman Phull, Kyu-Ree Dhong, Hye-Jin Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cordyceps militaris</i> anti-allergy fermentation Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/121c3f1d572e4e56b3122871d9ff14e6
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spelling	oai:doaj.org-article:121c3f1d572e4e56b3122871d9ff14e62021-11-25T18:34:37ZLactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model10.3390/nu131138492072-6643https://doaj.org/article/121c3f1d572e4e56b3122871d9ff14e62021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3849https://doaj.org/toc/2072-6643<i>Cordyceps militaris</i> (<i>C. militaris</i>) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharides, and antioxidants. To improve its bioactivity, we fermented <i>C. militaris</i> with a <i>Pediococcus pentosaceus</i> strain isolated from a salted small octopus (SC11). The current study aimed to evaluate whether <i>P. pentosaceus</i> (SC11) fermentation could enhance the anti-allergic potential of <i>C. militaris</i> cultured on germinated <i>Rhynchosia nulubilis</i> (GRC) against a type I hypersensitive reaction in in vitro and in vivo studies. Total antioxidant capacity and cordycepin content were significantly increased in GRC after SC11 fermentation. GRC-SC11 showed significantly enhanced anti-allergic responses by inhibiting immunoglobulin E (IgE)/antigen-induced degranulation in RBL-2H3 cells, compared to GRC. The results demonstrated the significant inhibition of phosphorylated spleen tyrosine kinase (Syk)/ p38/GRB2-associated binding protein 2 (Gab2)/c-jun in IgE/Ag-triggered RBL-2H3 cells. Furthermore, suppressed mRNA levels of interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) in IgE/Ag-activated RBL-2H3 cells were observed. GRC-SC11 significantly ameliorated IgE-induced allergic reactions by suppressing the ear swelling, vascular permeability, and inflammatory cell infiltration in passive cutaneous anaphylaxis (PCA) BALB/c mice. In conclusion, GRC fermented with <i>P.pentosaceus</i> exerted enhanced anti-allergic effects, and increased the cordycepin content and antioxidants potential compared to GRC. It can be used as bio-functional food in the prevention and management of type I allergic diseases.Abdul-Rehman PhullKyu-Ree DhongHye-Jin ParkMDPI AGarticle<i>Cordyceps militaris</i>anti-allergyfermentationNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3849, p 3849 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cordyceps militaris</i> anti-allergy fermentation Nutrition. Foods and food supply TX341-641
spellingShingle	<i>Cordyceps militaris</i> anti-allergy fermentation Nutrition. Foods and food supply TX341-641 Abdul-Rehman Phull Kyu-Ree Dhong Hye-Jin Park Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
description	<i>Cordyceps militaris</i> (<i>C. militaris</i>) has various biomedical applications in traditional oriental medicine for different diseases including inflammatory and immune-dysregulated diseases. It is a reservoir of nutritional components such as cordycepin, polysaccharides, and antioxidants. To improve its bioactivity, we fermented <i>C. militaris</i> with a <i>Pediococcus pentosaceus</i> strain isolated from a salted small octopus (SC11). The current study aimed to evaluate whether <i>P. pentosaceus</i> (SC11) fermentation could enhance the anti-allergic potential of <i>C. militaris</i> cultured on germinated <i>Rhynchosia nulubilis</i> (GRC) against a type I hypersensitive reaction in in vitro and in vivo studies. Total antioxidant capacity and cordycepin content were significantly increased in GRC after SC11 fermentation. GRC-SC11 showed significantly enhanced anti-allergic responses by inhibiting immunoglobulin E (IgE)/antigen-induced degranulation in RBL-2H3 cells, compared to GRC. The results demonstrated the significant inhibition of phosphorylated spleen tyrosine kinase (Syk)/ p38/GRB2-associated binding protein 2 (Gab2)/c-jun in IgE/Ag-triggered RBL-2H3 cells. Furthermore, suppressed mRNA levels of interleukin-4 (IL-4) and tumor necrosis factor-α (TNF-α) in IgE/Ag-activated RBL-2H3 cells were observed. GRC-SC11 significantly ameliorated IgE-induced allergic reactions by suppressing the ear swelling, vascular permeability, and inflammatory cell infiltration in passive cutaneous anaphylaxis (PCA) BALB/c mice. In conclusion, GRC fermented with <i>P.pentosaceus</i> exerted enhanced anti-allergic effects, and increased the cordycepin content and antioxidants potential compared to GRC. It can be used as bio-functional food in the prevention and management of type I allergic diseases.
format	article
author	Abdul-Rehman Phull Kyu-Ree Dhong Hye-Jin Park
author_facet	Abdul-Rehman Phull Kyu-Ree Dhong Hye-Jin Park
author_sort	Abdul-Rehman Phull
title	Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
title_short	Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
title_full	Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
title_fullStr	Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
title_full_unstemmed	Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model
title_sort	lactic acid bacteria fermented <i>cordyceps militaris</i> (grc-sc11) suppresses ige mediated mast cell activation and type i hypersensitive allergic murine model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/121c3f1d572e4e56b3122871d9ff14e6
work_keys_str_mv	AT abdulrehmanphull lacticacidbacteriafermentedicordycepsmilitarisigrcsc11suppressesigemediatedmastcellactivationandtypeihypersensitiveallergicmurinemodel AT kyureedhong lacticacidbacteriafermentedicordycepsmilitarisigrcsc11suppressesigemediatedmastcellactivationandtypeihypersensitiveallergicmurinemodel AT hyejinpark lacticacidbacteriafermentedicordycepsmilitarisigrcsc11suppressesigemediatedmastcellactivationandtypeihypersensitiveallergicmurinemodel
_version_	1718410936383963136

Lactic Acid Bacteria Fermented <i>Cordyceps militaris</i> (GRC-SC11) Suppresses IgE Mediated Mast Cell Activation and Type I Hypersensitive Allergic Murine Model

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