Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Jana Liskova,1 Oleg Babchenko,2 Marian Varga,2 Alexander Kromka,2 Daniel Hadraba,1 Zdenek Svindrych,1 Zuzana Burdikova,1 Lucie Bacakova1 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 2Institute of Physics, Academy of Sciences of the Czech Republic, Pr...

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Autores principales:	Liskova J, Babchenko O, Varga M, Kromka A, Hadraba D, Svindrych Z, Burdikova Z, Bacakova L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/121dbf20ba0a42feb08bb4279be56e58
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spelling	oai:doaj.org-article:121dbf20ba0a42feb08bb4279be56e582021-12-02T00:06:57ZOsteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films1178-2013https://doaj.org/article/121dbf20ba0a42feb08bb4279be56e582015-01-01T00:00:00Zhttp://www.dovepress.com/osteogenic-cell-differentiation-on-h-terminated-and-o-terminated-nanoc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jana Liskova,1 Oleg Babchenko,2 Marian Varga,2 Alexander Kromka,2 Daniel Hadraba,1 Zdenek Svindrych,1 Zuzana Burdikova,1 Lucie Bacakova1 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 2Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic Abstract: Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings. Keywords: nanocrystalline diamond film, osteoblast, Saos-2, collagen, SHGLiskova JBabchenko OVarga MKromka AHadraba DSvindrych ZBurdikova ZBacakova LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 869-884 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Liskova J Babchenko O Varga M Kromka A Hadraba D Svindrych Z Burdikova Z Bacakova L Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
description	Jana Liskova,1 Oleg Babchenko,2 Marian Varga,2 Alexander Kromka,2 Daniel Hadraba,1 Zdenek Svindrych,1 Zuzana Burdikova,1 Lucie Bacakova1 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic; 2Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic Abstract: Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings. Keywords: nanocrystalline diamond film, osteoblast, Saos-2, collagen, SHG
format	article
author	Liskova J Babchenko O Varga M Kromka A Hadraba D Svindrych Z Burdikova Z Bacakova L
author_facet	Liskova J Babchenko O Varga M Kromka A Hadraba D Svindrych Z Burdikova Z Bacakova L
author_sort	Liskova J
title	Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
title_short	Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
title_full	Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
title_fullStr	Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
title_full_unstemmed	Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films
title_sort	osteogenic cell differentiation on h-terminated and o-terminated nanocrystalline diamond films
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/121dbf20ba0a42feb08bb4279be56e58
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Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

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