Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin

Abstract Staphylococcus aureus is one of the main bacterial agents responsible for cases of mastitis in ruminants, playing an important role in the persistence and chronicity of diseases treated with antimicrobials. Using the multilocus sequence typing technique, network approaches and study of the...

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Autores principales:	Richard Costa Polveiro, Manuela Maria Cavalcante Granja, Thais Coimbra Borba Roldão, Ilderlane Da Silva Lopes, Pedro Marcus Pereira Vidigal, Magna Coroa Lima, Maria Aparecida Scatamburlo Moreira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/121f7432a1494fbcb9be7b18e81013ae
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spelling	oai:doaj.org-article:121f7432a1494fbcb9be7b18e81013ae2021-12-02T16:34:58ZMultilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin10.1038/s41598-021-96764-z2045-2322https://doaj.org/article/121f7432a1494fbcb9be7b18e81013ae2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96764-zhttps://doaj.org/toc/2045-2322Abstract Staphylococcus aureus is one of the main bacterial agents responsible for cases of mastitis in ruminants, playing an important role in the persistence and chronicity of diseases treated with antimicrobials. Using the multilocus sequence typing technique, network approaches and study of the population diversity of microorganisms, we performed analyzes of S. aureus (ES-GPM) isolated from goats with persistent mastitis (GPM). The most strains of ES-GPM were categorically different phylogenetically from the others and could be divided into two lineages: one with a majority belonging to ES-GPM and the other to varied strains. These two lineages were separated by 27 nuclear polymorphisms. The 43 strains comprised 22 clonal complexes (CCs), of which the ES-GPM strains were present in CC133, CC5 and a new complex formed by the sequence type 4966. The genetic diversity of some alleles showed be greater diversity and polymorphism than others, such as of the aroE and yqiL genes less than glpF gene. In addition, the sequences ES-GPM to the arc gene and glpF alleles showed the greatest number of mutations for ES-GPM in relation to non-ES-GPM. Therefore, this study identified genetic polymorphisms characteristic of S. aureus isolated from milk of goats diagnosed with persistent mastitis after the failed treatment with the antibiotic enrofloxacin. This study may help in the future to identify and discriminate this agent in cases of mastitis, and with that, the most appropriate antibiotic treatment can be performed in advance of the appearance of persistent mastitis caused by the agent, reducing the chances of premature culling and animal suffering.Richard Costa PolveiroManuela Maria Cavalcante GranjaThais Coimbra Borba RoldãoIlderlane Da Silva LopesPedro Marcus Pereira VidigalMagna Coroa LimaMaria Aparecida Scatamburlo MoreiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Richard Costa Polveiro Manuela Maria Cavalcante Granja Thais Coimbra Borba Roldão Ilderlane Da Silva Lopes Pedro Marcus Pereira Vidigal Magna Coroa Lima Maria Aparecida Scatamburlo Moreira Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
description	Abstract Staphylococcus aureus is one of the main bacterial agents responsible for cases of mastitis in ruminants, playing an important role in the persistence and chronicity of diseases treated with antimicrobials. Using the multilocus sequence typing technique, network approaches and study of the population diversity of microorganisms, we performed analyzes of S. aureus (ES-GPM) isolated from goats with persistent mastitis (GPM). The most strains of ES-GPM were categorically different phylogenetically from the others and could be divided into two lineages: one with a majority belonging to ES-GPM and the other to varied strains. These two lineages were separated by 27 nuclear polymorphisms. The 43 strains comprised 22 clonal complexes (CCs), of which the ES-GPM strains were present in CC133, CC5 and a new complex formed by the sequence type 4966. The genetic diversity of some alleles showed be greater diversity and polymorphism than others, such as of the aroE and yqiL genes less than glpF gene. In addition, the sequences ES-GPM to the arc gene and glpF alleles showed the greatest number of mutations for ES-GPM in relation to non-ES-GPM. Therefore, this study identified genetic polymorphisms characteristic of S. aureus isolated from milk of goats diagnosed with persistent mastitis after the failed treatment with the antibiotic enrofloxacin. This study may help in the future to identify and discriminate this agent in cases of mastitis, and with that, the most appropriate antibiotic treatment can be performed in advance of the appearance of persistent mastitis caused by the agent, reducing the chances of premature culling and animal suffering.
format	article
author	Richard Costa Polveiro Manuela Maria Cavalcante Granja Thais Coimbra Borba Roldão Ilderlane Da Silva Lopes Pedro Marcus Pereira Vidigal Magna Coroa Lima Maria Aparecida Scatamburlo Moreira
author_facet	Richard Costa Polveiro Manuela Maria Cavalcante Granja Thais Coimbra Borba Roldão Ilderlane Da Silva Lopes Pedro Marcus Pereira Vidigal Magna Coroa Lima Maria Aparecida Scatamburlo Moreira
author_sort	Richard Costa Polveiro
title	Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
title_short	Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
title_full	Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
title_fullStr	Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
title_full_unstemmed	Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
title_sort	multilocus sequence analysis reveals genetic diversity in staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/121f7432a1494fbcb9be7b18e81013ae
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Multilocus sequence analysis reveals genetic diversity in Staphylococcus aureus isolate of goat with mastitis persistent after treatment with enrofloxacin

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