IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC
Sugars are important molecules with remarkable cell signals pathway functions in higher organisms. The structural complexity of sugar represented by its isomeric, anomeric and diasteromeric configurations deserve the implementation of modern, rapid and sensitive methodologies for its identification...
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Universidad de Antioquia
2010
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oai:doaj.org-article:12219e5589e2425d943a60f5d7f844442021-11-19T04:14:36ZIDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC0121-40042145-2660https://doaj.org/article/12219e5589e2425d943a60f5d7f844442010-03-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/4982https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660Sugars are important molecules with remarkable cell signals pathway functions in higher organisms. The structural complexity of sugar represented by its isomeric, anomeric and diasteromeric configurations deserve the implementation of modern, rapid and sensitive methodologies for its identification and differentiation. Mass spectrometry and its analyzer of ion trap provide new alternative techniques that encourage the control of the fragmentation energies supplied to molecules. Since stereoisomer differentiation is consider outside the mass spectrometry domain, a methodology has been applied in order to differentiate β-D-galactose, β-D-glucose and the disaccharides β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside (lactose), α-D-glucopyranosyl-(1→4)-β-D-glucopyranoside (maltose) y β-D-fructofuranosyl-(2↔1)-α- D-glucopyranoside (sacarose) trough its ammonium and lithium adducts by infusion on ESI-IT-MS/MS mass spectrometer. Different fragmentation energies have been used to ensure the ion marker occurrence in the analyte stereochemistry. It is evident that the collision energies control in structural analysis of molecules provides a powerful and modern analytical tool to be applied in control laboratories.Guillermo L. MONTOYA P.Alejandra RENDÓN M.Gabriel J. ARANGO A.Universidad de Antioquiaarticleelectrosprayion trap analyzermonosaccharidesdisaccharidesdiasteromerstandem massFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 17, Iss 1 (2010) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
electrospray ion trap analyzer monosaccharides disaccharides diasteromers tandem mass Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 |
| spellingShingle |
electrospray ion trap analyzer monosaccharides disaccharides diasteromers tandem mass Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Guillermo L. MONTOYA P. Alejandra RENDÓN M. Gabriel J. ARANGO A. IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC |
| description |
Sugars are important molecules with remarkable cell signals pathway functions in higher organisms.
The structural complexity of sugar represented by its isomeric, anomeric and diasteromeric configurations
deserve the implementation of modern, rapid and sensitive methodologies for its identification and differentiation.
Mass spectrometry and its analyzer of ion trap provide new alternative techniques that encourage
the control of the fragmentation energies supplied to molecules. Since stereoisomer differentiation is
consider outside the mass spectrometry domain, a methodology has been applied in order to differentiate
β-D-galactose, β-D-glucose and the disaccharides β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside
(lactose), α-D-glucopyranosyl-(1→4)-β-D-glucopyranoside (maltose) y β-D-fructofuranosyl-(2↔1)-α-
D-glucopyranoside (sacarose) trough its ammonium and lithium adducts by infusion on ESI-IT-MS/MS
mass spectrometer. Different fragmentation energies have been used to ensure the ion marker occurrence
in the analyte stereochemistry. It is evident that the collision energies control in structural analysis of
molecules provides a powerful and modern analytical tool to be applied in control laboratories. |
| format |
article |
| author |
Guillermo L. MONTOYA P. Alejandra RENDÓN M. Gabriel J. ARANGO A. |
| author_facet |
Guillermo L. MONTOYA P. Alejandra RENDÓN M. Gabriel J. ARANGO A. |
| author_sort |
Guillermo L. MONTOYA P. |
| title |
IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC |
| title_short |
IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC |
| title_full |
IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC |
| title_fullStr |
IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC |
| title_full_unstemmed |
IDENTIFICATION AND DIFFERENTIATION OF UNDERIVATIZED DIASTEROMERIC |
| title_sort |
identification and differentiation of underivatized diasteromeric |
| publisher |
Universidad de Antioquia |
| publishDate |
2010 |
| url |
https://doaj.org/article/12219e5589e2425d943a60f5d7f84444 |
| work_keys_str_mv |
AT guillermolmontoyap identificationanddifferentiationofunderivatizeddiasteromeric AT alejandrarendonm identificationanddifferentiationofunderivatizeddiasteromeric AT gabrieljarangoa identificationanddifferentiationofunderivatizeddiasteromeric |
| _version_ |
1718420450433826816 |