Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

The genus Aeromonas has been recognised as an important pathogenic species in aquaculture that causes motile Aeromonas septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different Aeromonas spp....

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Autores principales:	Mohamad Azzam-Sayuti, Md Yasin Ina-Salwany, Mohd Zamri-Saad, Salleh Annas, Mohd Termizi Yusof, Md Shirajum Monir, Aslah Mohamad, Mohd Hafiz Ngoo Muhamad-Sofie, Jing Yie Lee, Yong Kit Chin, Zahaludin Amir-Danial, Addenan Asyiqin, Basri Lukman, Mark R. Liles, Mohammad Noor Azmai Amal
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>myo</i>-inositol LD<sub>50</sub> <i>Aeromonas</i> red hybrid tilapia pathogenicity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1225e12b725148a58d05d838cf480da6
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spelling	oai:doaj.org-article:1225e12b725148a58d05d838cf480da62021-11-25T16:47:51ZComparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)10.3390/biology101111922079-7737https://doaj.org/article/1225e12b725148a58d05d838cf480da62021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1192https://doaj.org/toc/2079-7737The genus <i>Aeromonas</i> has been recognised as an important pathogenic species in aquaculture that causes motile <i>Aeromonas</i> septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different <i>Aeromonas</i> spp. that were previously isolated from freshwater fish with signs of MAS. A total of 124 isolates of <i>Aeromonas</i> spp. were initially screened for the ability to grow on M9 agar with <i>myo</i>-inositol as a sole carbon source, which is a discriminatory phenotype for the hypervirulent <i>A. hydrophila</i> (vAh) pathotype. Subsequently, LD<sub>50</sub> of six selected <i>Aeromonas</i> spp<sub>.</sub> were determined by intraperitoneal injection of bacterial suspension containing 10<sup>3</sup>, 10<sup>5</sup>, and 10<sup>7</sup> CFU/mL of the respective <i>Aeromonas</i> sp. to red hybrid tilapias. The kidneys, livers and spleens of infected moribund fish were examined for histopathological changes. The screening revealed that only <i>A. dhakensis</i> 1P11S3 was able to grow using <i>myo</i>-inositol as a sole carbon source, and no vAh strains were identified. The LD<sub>50–240h</sub> of <i>A. dhakensis</i> 1P11S3 was 10<sup>7</sup> CFU/mL, while the non-<i>myo</i>-inositol utilizing <i>A. dhakensis</i> 4PS2 and <i>A. hydrophila</i> 8TK3 was lower at 10<sup>5</sup> CFU/mL. Similarly, tilapia challenged with the <i>myo</i>-inositol <i>A. dhakensis</i> 1P11S3 showed significantly (<i>p</i> < 0.05) less severe signs, gross and histopathological lesions, and a lower mortality rate than the non-<i>myo</i>-inositol <i>A. dhakensis</i> 4PS2 and <i>A. hydrophila</i> 8TK3. These findings suggested that <i>myo</i>-inositol utilizing <i>A. dhakensis</i> 1P11S3 was not a hypervirulent <i>Aeromonas</i> sp. under current experimental disease challenge conditions, and that diverse <i>Aeromonas</i> spp. are of concern in aquaculture farmed freshwater fish. Therefore, future study is warranted on genomic level to further elucidate the influence of <i>myo</i>-inositol utilizing ability on the pathogenesis of <i>Aeromonas</i> spp., since this ability correlates with hypervirulence in <i>A. hydrophila</i> strains.Mohamad Azzam-SayutiMd Yasin Ina-SalwanyMohd Zamri-SaadSalleh AnnasMohd Termizi YusofMd Shirajum MonirAslah MohamadMohd Hafiz Ngoo Muhamad-SofieJing Yie LeeYong Kit ChinZahaludin Amir-DanialAddenan AsyiqinBasri LukmanMark R. LilesMohammad Noor Azmai AmalMDPI AGarticle<i>myo</i>-inositolLD<sub>50</sub><i>Aeromonas</i>red hybrid tilapiapathogenicityBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1192, p 1192 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>myo</i>-inositol LD<sub>50</sub> <i>Aeromonas</i> red hybrid tilapia pathogenicity Biology (General) QH301-705.5
spellingShingle	<i>myo</i>-inositol LD<sub>50</sub> <i>Aeromonas</i> red hybrid tilapia pathogenicity Biology (General) QH301-705.5 Mohamad Azzam-Sayuti Md Yasin Ina-Salwany Mohd Zamri-Saad Salleh Annas Mohd Termizi Yusof Md Shirajum Monir Aslah Mohamad Mohd Hafiz Ngoo Muhamad-Sofie Jing Yie Lee Yong Kit Chin Zahaludin Amir-Danial Addenan Asyiqin Basri Lukman Mark R. Liles Mohammad Noor Azmai Amal Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)
description	The genus <i>Aeromonas</i> has been recognised as an important pathogenic species in aquaculture that causes motile <i>Aeromonas</i> septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different <i>Aeromonas</i> spp. that were previously isolated from freshwater fish with signs of MAS. A total of 124 isolates of <i>Aeromonas</i> spp. were initially screened for the ability to grow on M9 agar with <i>myo</i>-inositol as a sole carbon source, which is a discriminatory phenotype for the hypervirulent <i>A. hydrophila</i> (vAh) pathotype. Subsequently, LD<sub>50</sub> of six selected <i>Aeromonas</i> spp<sub>.</sub> were determined by intraperitoneal injection of bacterial suspension containing 10<sup>3</sup>, 10<sup>5</sup>, and 10<sup>7</sup> CFU/mL of the respective <i>Aeromonas</i> sp. to red hybrid tilapias. The kidneys, livers and spleens of infected moribund fish were examined for histopathological changes. The screening revealed that only <i>A. dhakensis</i> 1P11S3 was able to grow using <i>myo</i>-inositol as a sole carbon source, and no vAh strains were identified. The LD<sub>50–240h</sub> of <i>A. dhakensis</i> 1P11S3 was 10<sup>7</sup> CFU/mL, while the non-<i>myo</i>-inositol utilizing <i>A. dhakensis</i> 4PS2 and <i>A. hydrophila</i> 8TK3 was lower at 10<sup>5</sup> CFU/mL. Similarly, tilapia challenged with the <i>myo</i>-inositol <i>A. dhakensis</i> 1P11S3 showed significantly (<i>p</i> < 0.05) less severe signs, gross and histopathological lesions, and a lower mortality rate than the non-<i>myo</i>-inositol <i>A. dhakensis</i> 4PS2 and <i>A. hydrophila</i> 8TK3. These findings suggested that <i>myo</i>-inositol utilizing <i>A. dhakensis</i> 1P11S3 was not a hypervirulent <i>Aeromonas</i> sp. under current experimental disease challenge conditions, and that diverse <i>Aeromonas</i> spp. are of concern in aquaculture farmed freshwater fish. Therefore, future study is warranted on genomic level to further elucidate the influence of <i>myo</i>-inositol utilizing ability on the pathogenesis of <i>Aeromonas</i> spp., since this ability correlates with hypervirulence in <i>A. hydrophila</i> strains.
format	article
author	Mohamad Azzam-Sayuti Md Yasin Ina-Salwany Mohd Zamri-Saad Salleh Annas Mohd Termizi Yusof Md Shirajum Monir Aslah Mohamad Mohd Hafiz Ngoo Muhamad-Sofie Jing Yie Lee Yong Kit Chin Zahaludin Amir-Danial Addenan Asyiqin Basri Lukman Mark R. Liles Mohammad Noor Azmai Amal
author_facet	Mohamad Azzam-Sayuti Md Yasin Ina-Salwany Mohd Zamri-Saad Salleh Annas Mohd Termizi Yusof Md Shirajum Monir Aslah Mohamad Mohd Hafiz Ngoo Muhamad-Sofie Jing Yie Lee Yong Kit Chin Zahaludin Amir-Danial Addenan Asyiqin Basri Lukman Mark R. Liles Mohammad Noor Azmai Amal
author_sort	Mohamad Azzam-Sayuti
title	Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)
title_short	Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)
title_full	Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)
title_fullStr	Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)
title_full_unstemmed	Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)
title_sort	comparative pathogenicity of <i>aeromonas</i> spp. in cultured red hybrid tilapia (<i>oreochromis niloticus × o. mossambicus</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1225e12b725148a58d05d838cf480da6
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Comparative Pathogenicity of <i>Aeromonas</i> spp. in Cultured Red Hybrid Tilapia (<i>Oreochromis niloticus × O. mossambicus</i>)

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