New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum.
Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-thr...
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2006
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oai:doaj.org-article:122ae105492b416498ac8685be73caf62021-12-02T19:59:51ZNew perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum.1553-73661553-737410.1371/journal.ppat.0020029https://doaj.org/article/122ae105492b416498ac8685be73caf62006-04-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.0020029https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay.Feng LiuJiong LuWei HuSheng-Yue WangShu-Jian CuiMing ChiQing YanXin-Rong WangHuai-Dong SongXue-Nian XuJu-Jun WangXiang-Lin ZhangXin ZhangZhi-Qin WangChun-Liang XuePaul J BrindleyDonald P McManusPeng-Yuan YangZheng FengZhu ChenZe-Guang HanPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 2, Iss 4, p e29 (2006) |
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EN |
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Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Feng Liu Jiong Lu Wei Hu Sheng-Yue Wang Shu-Jian Cui Ming Chi Qing Yan Xin-Rong Wang Huai-Dong Song Xue-Nian Xu Ju-Jun Wang Xiang-Lin Zhang Xin Zhang Zhi-Qin Wang Chun-Liang Xue Paul J Brindley Donald P McManus Peng-Yuan Yang Zheng Feng Zhu Chen Ze-Guang Han New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. |
| description |
Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay. |
| format |
article |
| author |
Feng Liu Jiong Lu Wei Hu Sheng-Yue Wang Shu-Jian Cui Ming Chi Qing Yan Xin-Rong Wang Huai-Dong Song Xue-Nian Xu Ju-Jun Wang Xiang-Lin Zhang Xin Zhang Zhi-Qin Wang Chun-Liang Xue Paul J Brindley Donald P McManus Peng-Yuan Yang Zheng Feng Zhu Chen Ze-Guang Han |
| author_facet |
Feng Liu Jiong Lu Wei Hu Sheng-Yue Wang Shu-Jian Cui Ming Chi Qing Yan Xin-Rong Wang Huai-Dong Song Xue-Nian Xu Ju-Jun Wang Xiang-Lin Zhang Xin Zhang Zhi-Qin Wang Chun-Liang Xue Paul J Brindley Donald P McManus Peng-Yuan Yang Zheng Feng Zhu Chen Ze-Guang Han |
| author_sort |
Feng Liu |
| title |
New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. |
| title_short |
New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. |
| title_full |
New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. |
| title_fullStr |
New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. |
| title_full_unstemmed |
New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. |
| title_sort |
new perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of schistosoma japonicum. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2006 |
| url |
https://doaj.org/article/122ae105492b416498ac8685be73caf6 |
| work_keys_str_mv |
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| _version_ |
1718375704225120256 |