The impact of liposomal linolenic acid on gastrointestinal microbiota in mice

Xuan-xuan Li,1 Si Shi,2 Lan Rong,1 Mei-qing Feng,2 Liang Zhong1 1Department of Digestive Diseases, Huashan Hospital Affiliated to Fudan University, Shanghai, China; 2School of Pharmacy, Fudan University, Shanghai, China Background: The prevalence of Helicobacter pylori has long been a global healt...

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Autores principales:	Li XX, Shi S, Rong L, Feng MQ, Zhong L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	nanomedicine linolenic acid Helicobacter pylori gastric microbiota gut microbiota Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/12302b12c131403290da89021bb7bbc8
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spelling	oai:doaj.org-article:12302b12c131403290da89021bb7bbc82021-12-02T08:30:33ZThe impact of liposomal linolenic acid on gastrointestinal microbiota in mice1178-2013https://doaj.org/article/12302b12c131403290da89021bb7bbc82018-03-01T00:00:00Zhttps://www.dovepress.com/the-impact-of-liposomal-linolenic-acid-on-gastrointestinal-microbiota--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xuan-xuan Li,1 Si Shi,2 Lan Rong,1 Mei-qing Feng,2 Liang Zhong1 1Department of Digestive Diseases, Huashan Hospital Affiliated to Fudan University, Shanghai, China; 2School of Pharmacy, Fudan University, Shanghai, China Background: The prevalence of Helicobacter pylori has long been a global health issue. Triple therapy, being the first-line treatment, has caused dysbiosis of the gastrointestinal tract that led to various complications. A novel nanomedicine – liposomal linolenic acid (LipoLLA) – has been proven to have great potential in eradicating H. pylori. However, the possible side effects of LipoLLA due to alteration of the gastrointestinal microbiota remain unknown.Aim: This study focused on the impact of LipoLLA on gastrointestinal microbiota in mice in comparison with triple therapy in order to assess the safety profile.Methods: Mice were divided into five groups: blank control group; H. pylori control group; triple therapy group; low-dose LipoLLA group (25 mg/kg); and high-dose LipoLLA group (50 mg/kg). Fecal samples were collected before and after the intake of corresponding formulas. Gastric tissues were obtained after mice dissection. These samples were analyzed with high-throughput sequencing.Results: The analysis revealed that LipoLLA resulted in minor gut microbiota alteration at different levels. The altered proportions in the high-dose group were higher than that of the low-dose group. On the other hand, the triple therapy group showed dramatic shifts in the major community composition. It displayed a notable boost in the relative abundance of Proteobacteria and Firmicutes along with a decrease in that of Verrucomicrobia and Bacteroidetes. All of them belonged to the major phyla in the microbiome. Triple therapy also led to the growth of the family Enterobacteriaceae, Enterococcaceae, and Clostridiaceae_1 that may be associated with clinical illnesses. Gastric microbiota analysis reached similar conclusions.Conclusion: Our findings indicated that LipoLLA causes minor gastrointestinal microbiota alterations while the triple therapy triggered dramatic changes. Thus, LipoLLA is not only promising but also a safe therapeutic medication to eradicate H. pylori infection. Keywords: nanomedicine, linolenic acid, Helicobacter pylori, gastric microbiota, gut microbiotaLi XXShi SRong LFeng MQZhong LDove Medical Pressarticlenanomedicinelinolenic acidHelicobacter pylorigastric microbiotagut microbiotaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1399-1409 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanomedicine linolenic acid Helicobacter pylori gastric microbiota gut microbiota Medicine (General) R5-920
spellingShingle	nanomedicine linolenic acid Helicobacter pylori gastric microbiota gut microbiota Medicine (General) R5-920 Li XX Shi S Rong L Feng MQ Zhong L The impact of liposomal linolenic acid on gastrointestinal microbiota in mice
description	Xuan-xuan Li,1 Si Shi,2 Lan Rong,1 Mei-qing Feng,2 Liang Zhong1 1Department of Digestive Diseases, Huashan Hospital Affiliated to Fudan University, Shanghai, China; 2School of Pharmacy, Fudan University, Shanghai, China Background: The prevalence of Helicobacter pylori has long been a global health issue. Triple therapy, being the first-line treatment, has caused dysbiosis of the gastrointestinal tract that led to various complications. A novel nanomedicine – liposomal linolenic acid (LipoLLA) – has been proven to have great potential in eradicating H. pylori. However, the possible side effects of LipoLLA due to alteration of the gastrointestinal microbiota remain unknown.Aim: This study focused on the impact of LipoLLA on gastrointestinal microbiota in mice in comparison with triple therapy in order to assess the safety profile.Methods: Mice were divided into five groups: blank control group; H. pylori control group; triple therapy group; low-dose LipoLLA group (25 mg/kg); and high-dose LipoLLA group (50 mg/kg). Fecal samples were collected before and after the intake of corresponding formulas. Gastric tissues were obtained after mice dissection. These samples were analyzed with high-throughput sequencing.Results: The analysis revealed that LipoLLA resulted in minor gut microbiota alteration at different levels. The altered proportions in the high-dose group were higher than that of the low-dose group. On the other hand, the triple therapy group showed dramatic shifts in the major community composition. It displayed a notable boost in the relative abundance of Proteobacteria and Firmicutes along with a decrease in that of Verrucomicrobia and Bacteroidetes. All of them belonged to the major phyla in the microbiome. Triple therapy also led to the growth of the family Enterobacteriaceae, Enterococcaceae, and Clostridiaceae_1 that may be associated with clinical illnesses. Gastric microbiota analysis reached similar conclusions.Conclusion: Our findings indicated that LipoLLA causes minor gastrointestinal microbiota alterations while the triple therapy triggered dramatic changes. Thus, LipoLLA is not only promising but also a safe therapeutic medication to eradicate H. pylori infection. Keywords: nanomedicine, linolenic acid, Helicobacter pylori, gastric microbiota, gut microbiota
format	article
author	Li XX Shi S Rong L Feng MQ Zhong L
author_facet	Li XX Shi S Rong L Feng MQ Zhong L
author_sort	Li XX
title	The impact of liposomal linolenic acid on gastrointestinal microbiota in mice
title_short	The impact of liposomal linolenic acid on gastrointestinal microbiota in mice
title_full	The impact of liposomal linolenic acid on gastrointestinal microbiota in mice
title_fullStr	The impact of liposomal linolenic acid on gastrointestinal microbiota in mice
title_full_unstemmed	The impact of liposomal linolenic acid on gastrointestinal microbiota in mice
title_sort	impact of liposomal linolenic acid on gastrointestinal microbiota in mice
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/12302b12c131403290da89021bb7bbc8
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The impact of liposomal linolenic acid on gastrointestinal microbiota in mice

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