Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells

Abstract Ovarian cancer is associated with a high mortality rate due to diagnosis at advanced stages. Dissemination often occurs intraperitoneally within the ascites fluid. The microenvironment can support dissemination through several mechanisms. One potential ascites factor which may mediate disse...

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Autores principales:	Aparna Mitra, Kyoko Yoshida-Court, Travis N. Solley, Megan Mikkelson, Chi Lam Au Yeung, Alpa Nick, Karen Lu, Ann H. Klopp
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/123098e21ab7433faca1d8b0af444bd0
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spelling	oai:doaj.org-article:123098e21ab7433faca1d8b0af444bd02021-12-02T13:41:22ZExtracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells10.1038/s41598-021-88163-12045-2322https://doaj.org/article/123098e21ab7433faca1d8b0af444bd02021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88163-1https://doaj.org/toc/2045-2322Abstract Ovarian cancer is associated with a high mortality rate due to diagnosis at advanced stages. Dissemination often occurs intraperitoneally within the ascites fluid. The microenvironment can support dissemination through several mechanisms. One potential ascites factor which may mediate dissemination are EVs or extracellular vesicles that can carry information in the form of miRNAs, proteins, lipids, and act as mediators of cellular communication. We present our observations on EVs isolated from ascitic supernatants from patients diagnosed with high grade serous ovarian carcinoma in augmenting motility, growth, and migration towards omental fat. MicroRNA profiling of EVs from malignant ascitic supernatant demonstrates high expression of miR 200c-3p, miR18a-5p, miR1246, and miR1290 and low expression of miR 100- 5p as compared to EVs isolated from benign ascitic supernatant. The migration of ovarian cancer spheroids towards omental fat is enhanced in the presence of malignant ascitic EVs. Gene expression of these cells showed increased expression of ZBED2, ZBTB20, ABCC3, UHMK1, and low expression of Transgelin and MARCKS. We present evidence that ovarian ascitic EVs increase the growth of ovarian cancer spheroids through miRNAs.Aparna MitraKyoko Yoshida-CourtTravis N. SolleyMegan MikkelsonChi Lam Au YeungAlpa NickKaren LuAnn H. KloppNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aparna Mitra Kyoko Yoshida-Court Travis N. Solley Megan Mikkelson Chi Lam Au Yeung Alpa Nick Karen Lu Ann H. Klopp Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
description	Abstract Ovarian cancer is associated with a high mortality rate due to diagnosis at advanced stages. Dissemination often occurs intraperitoneally within the ascites fluid. The microenvironment can support dissemination through several mechanisms. One potential ascites factor which may mediate dissemination are EVs or extracellular vesicles that can carry information in the form of miRNAs, proteins, lipids, and act as mediators of cellular communication. We present our observations on EVs isolated from ascitic supernatants from patients diagnosed with high grade serous ovarian carcinoma in augmenting motility, growth, and migration towards omental fat. MicroRNA profiling of EVs from malignant ascitic supernatant demonstrates high expression of miR 200c-3p, miR18a-5p, miR1246, and miR1290 and low expression of miR 100- 5p as compared to EVs isolated from benign ascitic supernatant. The migration of ovarian cancer spheroids towards omental fat is enhanced in the presence of malignant ascitic EVs. Gene expression of these cells showed increased expression of ZBED2, ZBTB20, ABCC3, UHMK1, and low expression of Transgelin and MARCKS. We present evidence that ovarian ascitic EVs increase the growth of ovarian cancer spheroids through miRNAs.
format	article
author	Aparna Mitra Kyoko Yoshida-Court Travis N. Solley Megan Mikkelson Chi Lam Au Yeung Alpa Nick Karen Lu Ann H. Klopp
author_facet	Aparna Mitra Kyoko Yoshida-Court Travis N. Solley Megan Mikkelson Chi Lam Au Yeung Alpa Nick Karen Lu Ann H. Klopp
author_sort	Aparna Mitra
title	Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
title_short	Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
title_full	Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
title_fullStr	Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
title_full_unstemmed	Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
title_sort	extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/123098e21ab7433faca1d8b0af444bd0
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Extracellular vesicles derived from ascitic fluid enhance growth and migration of ovarian cancer cells

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