MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel

The red coloration of apple (Malus × domestica Borkh.) is due to the accumulation of anthocyanins in the fruit peel. Light is essential for anthocyanin biosynthesis in apple. In this study, we performed a transcriptome sequencing (RNA-seq) analysis of apple fruit exposed to light after unbagging. Th...

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Autores principales:	Bo Zhang, Zhen-Zhen Zhu, Dong Qu, Bo-Chen Wang, Ni-Ni Hao, Ya-Zhou Yang, Hui-Juan Yang, Zheng-Yang Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	apple light anthocyanin MdBBX21 MdMYB1 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/12348ecfdd154c52970fd19cd6d0a0ac
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spelling	oai:doaj.org-article:12348ecfdd154c52970fd19cd6d0a0ac2021-11-12T06:02:13ZMdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel1664-462X10.3389/fpls.2021.774446https://doaj.org/article/12348ecfdd154c52970fd19cd6d0a0ac2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.774446/fullhttps://doaj.org/toc/1664-462XThe red coloration of apple (Malus × domestica Borkh.) is due to the accumulation of anthocyanins in the fruit peel. Light is essential for anthocyanin biosynthesis in apple. In this study, we performed a transcriptome sequencing (RNA-seq) analysis of apple fruit exposed to light after unbagging. The identified differentially expressed genes included MdBBX21, which is homologous to Arabidopsis BBX21, suggesting it may be involved in light-induced anthocyanin biosynthesis. Additionally, MdBBX21 was localized in the nucleus and its gene was expressed earlier than MdMYB1 in apple peel treated with light. Overexpressing MdBBX21 in Arabidopsis and apple calli under light increased anthocyanin accumulation. Dual-luciferase and yeast one-hybrid assays confirmed that MdBBX21 binds to the MdHY5, MdBBX20, and MdBBX22-1/2 promoters and induces expression. At the same time, MdHY5 can also activate the expression of MdBBX21. Furthermore, bimolecular fluorescence complementation and yeast two-hybrid assays demonstrated that MdBBX21 can interact with MdHY5. This interaction can significantly enhance MdMYB1 promoter activity. These findings clarify the molecular mechanism by which MdBBX21 positively regulates light-induced anthocyanin accumulation in apple.Bo ZhangBo ZhangZhen-Zhen ZhuZhen-Zhen ZhuDong QuBo-Chen WangBo-Chen WangNi-Ni HaoNi-Ni HaoYa-Zhou YangYa-Zhou YangHui-Juan YangHui-Juan YangZheng-Yang ZhaoZheng-Yang ZhaoFrontiers Media S.A.articleapplelightanthocyaninMdBBX21MdMYB1Plant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apple light anthocyanin MdBBX21 MdMYB1 Plant culture SB1-1110
spellingShingle	apple light anthocyanin MdBBX21 MdMYB1 Plant culture SB1-1110 Bo Zhang Bo Zhang Zhen-Zhen Zhu Zhen-Zhen Zhu Dong Qu Bo-Chen Wang Bo-Chen Wang Ni-Ni Hao Ni-Ni Hao Ya-Zhou Yang Ya-Zhou Yang Hui-Juan Yang Hui-Juan Yang Zheng-Yang Zhao Zheng-Yang Zhao MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel
description	The red coloration of apple (Malus × domestica Borkh.) is due to the accumulation of anthocyanins in the fruit peel. Light is essential for anthocyanin biosynthesis in apple. In this study, we performed a transcriptome sequencing (RNA-seq) analysis of apple fruit exposed to light after unbagging. The identified differentially expressed genes included MdBBX21, which is homologous to Arabidopsis BBX21, suggesting it may be involved in light-induced anthocyanin biosynthesis. Additionally, MdBBX21 was localized in the nucleus and its gene was expressed earlier than MdMYB1 in apple peel treated with light. Overexpressing MdBBX21 in Arabidopsis and apple calli under light increased anthocyanin accumulation. Dual-luciferase and yeast one-hybrid assays confirmed that MdBBX21 binds to the MdHY5, MdBBX20, and MdBBX22-1/2 promoters and induces expression. At the same time, MdHY5 can also activate the expression of MdBBX21. Furthermore, bimolecular fluorescence complementation and yeast two-hybrid assays demonstrated that MdBBX21 can interact with MdHY5. This interaction can significantly enhance MdMYB1 promoter activity. These findings clarify the molecular mechanism by which MdBBX21 positively regulates light-induced anthocyanin accumulation in apple.
format	article
author	Bo Zhang Bo Zhang Zhen-Zhen Zhu Zhen-Zhen Zhu Dong Qu Bo-Chen Wang Bo-Chen Wang Ni-Ni Hao Ni-Ni Hao Ya-Zhou Yang Ya-Zhou Yang Hui-Juan Yang Hui-Juan Yang Zheng-Yang Zhao Zheng-Yang Zhao
author_facet	Bo Zhang Bo Zhang Zhen-Zhen Zhu Zhen-Zhen Zhu Dong Qu Bo-Chen Wang Bo-Chen Wang Ni-Ni Hao Ni-Ni Hao Ya-Zhou Yang Ya-Zhou Yang Hui-Juan Yang Hui-Juan Yang Zheng-Yang Zhao Zheng-Yang Zhao
author_sort	Bo Zhang
title	MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel
title_short	MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel
title_full	MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel
title_fullStr	MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel
title_full_unstemmed	MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel
title_sort	mdbbx21, a b-box protein, positively regulates light-induced anthocyanin accumulation in apple peel
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/12348ecfdd154c52970fd19cd6d0a0ac
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MdBBX21, a B-Box Protein, Positively Regulates Light-Induced Anthocyanin Accumulation in Apple Peel

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