Droplet-based microfluidics platform for antifungal analysis against filamentous fungi

Abstract Fungicides are extensively used in agriculture to control fungal pathogens which are responsible for significant economic impact on plant yield and quality. The conventional antifungal screening techniques, such as water agar and 96-well plates, are based on laborious protocols and bulk ana...

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Autores principales:	Sehrish Iftikhar, Aurélie Vigne, Julia Elisa Sepulveda-Diaz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/124631c916df4296a6c22a4b28168b28
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spelling	oai:doaj.org-article:124631c916df4296a6c22a4b28168b282021-11-28T12:18:56ZDroplet-based microfluidics platform for antifungal analysis against filamentous fungi10.1038/s41598-021-02350-82045-2322https://doaj.org/article/124631c916df4296a6c22a4b28168b282021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02350-8https://doaj.org/toc/2045-2322Abstract Fungicides are extensively used in agriculture to control fungal pathogens which are responsible for significant economic impact on plant yield and quality. The conventional antifungal screening techniques, such as water agar and 96-well plates, are based on laborious protocols and bulk analysis, restricting the analysis at the single spore level and are time consuming. In this study, we present a droplet-based microfluidic platform that enables antifungal analysis of single spores of filamentous fungus Alternaria alternata. A droplet-based viability assay was developed, allowing the germination and hyphal growth of single A. alternata spores within droplets. The viability was demonstrated over a period of 24 h and the antifungal screening was achieved using Kunshi/Tezuma as antifungal agent. The efficacy results of the droplet-based antifungal analysis were compared and validated with the results obtained from conventional protocols. The percentage inhibitions assessed by the droplet-based platform were equivalent with those obtained by the other two methods, and the Pearson correlation analysis showed high correlation between the three assays. Taken together, this droplet-based microfluidic platform provides a wide range of potential applications for the analysis of fungicide resistance development as well as combinatorial screening of other antimicrobial agents and even antagonistic fungi.Sehrish IftikharAurélie VigneJulia Elisa Sepulveda-DiazNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sehrish Iftikhar Aurélie Vigne Julia Elisa Sepulveda-Diaz Droplet-based microfluidics platform for antifungal analysis against filamentous fungi
description	Abstract Fungicides are extensively used in agriculture to control fungal pathogens which are responsible for significant economic impact on plant yield and quality. The conventional antifungal screening techniques, such as water agar and 96-well plates, are based on laborious protocols and bulk analysis, restricting the analysis at the single spore level and are time consuming. In this study, we present a droplet-based microfluidic platform that enables antifungal analysis of single spores of filamentous fungus Alternaria alternata. A droplet-based viability assay was developed, allowing the germination and hyphal growth of single A. alternata spores within droplets. The viability was demonstrated over a period of 24 h and the antifungal screening was achieved using Kunshi/Tezuma as antifungal agent. The efficacy results of the droplet-based antifungal analysis were compared and validated with the results obtained from conventional protocols. The percentage inhibitions assessed by the droplet-based platform were equivalent with those obtained by the other two methods, and the Pearson correlation analysis showed high correlation between the three assays. Taken together, this droplet-based microfluidic platform provides a wide range of potential applications for the analysis of fungicide resistance development as well as combinatorial screening of other antimicrobial agents and even antagonistic fungi.
format	article
author	Sehrish Iftikhar Aurélie Vigne Julia Elisa Sepulveda-Diaz
author_facet	Sehrish Iftikhar Aurélie Vigne Julia Elisa Sepulveda-Diaz
author_sort	Sehrish Iftikhar
title	Droplet-based microfluidics platform for antifungal analysis against filamentous fungi
title_short	Droplet-based microfluidics platform for antifungal analysis against filamentous fungi
title_full	Droplet-based microfluidics platform for antifungal analysis against filamentous fungi
title_fullStr	Droplet-based microfluidics platform for antifungal analysis against filamentous fungi
title_full_unstemmed	Droplet-based microfluidics platform for antifungal analysis against filamentous fungi
title_sort	droplet-based microfluidics platform for antifungal analysis against filamentous fungi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/124631c916df4296a6c22a4b28168b28
work_keys_str_mv	AT sehrishiftikhar dropletbasedmicrofluidicsplatformforantifungalanalysisagainstfilamentousfungi AT aurelievigne dropletbasedmicrofluidicsplatformforantifungalanalysisagainstfilamentousfungi AT juliaelisasepulvedadiaz dropletbasedmicrofluidicsplatformforantifungalanalysisagainstfilamentousfungi
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Droplet-based microfluidics platform for antifungal analysis against filamentous fungi

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