Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming

Johne’s disease, caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), causes weight loss, diarrhoea, and reduced milk yields in clinically infected cattle. Asymptomatic, subclinically infected cattle shed MAP bacteria but are frequently not detected by d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Emma N. Taylor, Manfred Beckmann, Bernardo Villarreal-Ramos, Hans-Martin Vordermeier, Glyn Hewinson, David Rooke, Luis A. J. Mur, Ad P. Koets
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> metabolomics antibody MAP antigens eicosanoids inflammation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/124b358003d34bcaab4b0538cc436520
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:124b358003d34bcaab4b0538cc436520
record_format	dspace
spelling	oai:doaj.org-article:124b358003d34bcaab4b0538cc4365202021-11-25T18:20:28ZMetabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming10.3390/metabo111107272218-1989https://doaj.org/article/124b358003d34bcaab4b0538cc4365202021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/727https://doaj.org/toc/2218-1989Johne’s disease, caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), causes weight loss, diarrhoea, and reduced milk yields in clinically infected cattle. Asymptomatic, subclinically infected cattle shed MAP bacteria but are frequently not detected by diagnostic tests. Herein, we compare the metabolite profiles of sera from subclinically infected Holstein–Friesian heifers and antibody binding to selected MAP antigens. The study used biobanked serum samples from 10 naturally MAP-infected and 10 control heifers, sampled monthly from ~1 to 19 months of age. Sera were assessed using flow infusion electrospray–high-resolution mass spectrometry (FIE–HRMS) on a Q Exactive hybrid quadrupole–Orbitrap mass spectrometer for high-throughput, sensitive, non-targeted metabolite fingerprinting. Partial least-squares discriminant analyses (PLS-DA) and hierarchical cluster analysis (HCA) of the data discriminated between naturally MAP-infected and control heifers. In total, 33 metabolites that differentially accumulated in naturally MAP-infected heifers compared to controls were identified. Five were significantly elevated within MAP-infected heifers throughout the study, i.e., leukotriene B4, bicyclo prostaglandin E2 (bicyclo PGE2), itaconic acid, 2-hydroxyglutaric acid and N6-acetyl-L-lysine. These findings highlight the potential of metabolomics in the identification of novel MAP diagnostic markers and particular biochemical pathways, which may provide insights into the bovine immune response to MAP.Emma N. TaylorManfred BeckmannBernardo Villarreal-RamosHans-Martin VordermeierGlyn HewinsonDavid RookeLuis A. J. MurAd P. KoetsMDPI AGarticle<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i>metabolomicsantibodyMAP antigenseicosanoidsinflammationMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 727, p 727 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> metabolomics antibody MAP antigens eicosanoids inflammation Microbiology QR1-502
spellingShingle	<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> metabolomics antibody MAP antigens eicosanoids inflammation Microbiology QR1-502 Emma N. Taylor Manfred Beckmann Bernardo Villarreal-Ramos Hans-Martin Vordermeier Glyn Hewinson David Rooke Luis A. J. Mur Ad P. Koets Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming
description	Johne’s disease, caused by <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP), causes weight loss, diarrhoea, and reduced milk yields in clinically infected cattle. Asymptomatic, subclinically infected cattle shed MAP bacteria but are frequently not detected by diagnostic tests. Herein, we compare the metabolite profiles of sera from subclinically infected Holstein–Friesian heifers and antibody binding to selected MAP antigens. The study used biobanked serum samples from 10 naturally MAP-infected and 10 control heifers, sampled monthly from ~1 to 19 months of age. Sera were assessed using flow infusion electrospray–high-resolution mass spectrometry (FIE–HRMS) on a Q Exactive hybrid quadrupole–Orbitrap mass spectrometer for high-throughput, sensitive, non-targeted metabolite fingerprinting. Partial least-squares discriminant analyses (PLS-DA) and hierarchical cluster analysis (HCA) of the data discriminated between naturally MAP-infected and control heifers. In total, 33 metabolites that differentially accumulated in naturally MAP-infected heifers compared to controls were identified. Five were significantly elevated within MAP-infected heifers throughout the study, i.e., leukotriene B4, bicyclo prostaglandin E2 (bicyclo PGE2), itaconic acid, 2-hydroxyglutaric acid and N6-acetyl-L-lysine. These findings highlight the potential of metabolomics in the identification of novel MAP diagnostic markers and particular biochemical pathways, which may provide insights into the bovine immune response to MAP.
format	article
author	Emma N. Taylor Manfred Beckmann Bernardo Villarreal-Ramos Hans-Martin Vordermeier Glyn Hewinson David Rooke Luis A. J. Mur Ad P. Koets
author_facet	Emma N. Taylor Manfred Beckmann Bernardo Villarreal-Ramos Hans-Martin Vordermeier Glyn Hewinson David Rooke Luis A. J. Mur Ad P. Koets
author_sort	Emma N. Taylor
title	Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming
title_short	Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming
title_full	Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming
title_fullStr	Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming
title_full_unstemmed	Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming
title_sort	metabolomic changes in naturally map-infected holstein–friesian heifers indicate immunologically related biochemical reprogramming
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/124b358003d34bcaab4b0538cc436520
work_keys_str_mv	AT emmantaylor metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT manfredbeckmann metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT bernardovillarrealramos metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT hansmartinvordermeier metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT glynhewinson metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT davidrooke metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT luisajmur metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming AT adpkoets metabolomicchangesinnaturallymapinfectedholsteinfriesianheifersindicateimmunologicallyrelatedbiochemicalreprogramming
_version_	1718411303984300032

Metabolomic Changes in Naturally MAP-Infected Holstein–Friesian Heifers Indicate Immunologically Related Biochemical Reprogramming

Ejemplares similares