Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging

AIM: To assess the protective effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exs) in a diabetic rat model by using a variety of retinal bioassays. METHODS: hucMSCs were subjected to differential ultracentrifugation for the collection of exosomes, and transmission electron micr...

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Autores principales:	Yan Fu, Xiang Gao, Guang-Hui He, Song Chen, Zhao-Hui Gu, Yue-Ling Zhang, Li-Ying Li
Formato:	article
Lenguaje:	EN
Publicado:	Press of International Journal of Ophthalmology (IJO PRESS) 2021
Materias:	human umbilical cord mesenchymal stem cells exosomes diabetic retinopathy fundus fluorescein angiography optical coherence tomography rat Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/124e15ac92f642bd8ebd4dc6fcd37a85
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spelling	oai:doaj.org-article:124e15ac92f642bd8ebd4dc6fcd37a852021-11-26T04:02:12ZProtective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging2222-39592227-489810.18240/ijo.2021.12.04https://doaj.org/article/124e15ac92f642bd8ebd4dc6fcd37a852021-12-01T00:00:00Zhttp://ies.ijo.cn/en_publish/2021/12/20211204.pdfhttps://doaj.org/toc/2222-3959https://doaj.org/toc/2227-4898AIM: To assess the protective effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exs) in a diabetic rat model by using a variety of retinal bioassays. METHODS: hucMSCs were subjected to differential ultracentrifugation for the collection of exosomes, and transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) using a NanoSight analysis system and Western blotting (WB) were used to analyze the expression of surface marker proteins such as CD63, CD9 and Calnexin. Streptozotocin (STZ) was injected into the intraperitoneal cavity to establish a diabetic model. Rats were divided into a normal group, diabetic group and hucMSC-Ex group. Fundus fluorescein angiography (FFA), optical coherence tomography (OCT) and other live imaging methods were used to observe the fundus of the rats. Finally, the eyeballs of rats from each group were collected for hematoxylin-eosin (HE) staining to further analyze the retinal structure. RESULTS: Through TEM, NTA and WB, we successfully isolated hucMSC-Exs. Subsequent FFA and OCT confirmed that hucMSC-Exs effectively prevented early retinal vascular damage and thickening of the retina. Finally, HE staining of rat retinal sections revealed that exosomes effectively alleviated retinal structure disruption caused by diabetes. CONCLUSION: hucMSC-Exs have a protective effect on the retina in diabetic rat through FFA, OCT and HE staining.Yan FuXiang GaoGuang-Hui HeSong ChenZhao-Hui GuYue-Ling ZhangLi-Ying LiPress of International Journal of Ophthalmology (IJO PRESS)articlehuman umbilical cord mesenchymal stem cellsexosomesdiabetic retinopathyfundus fluorescein angiographyoptical coherence tomographyratOphthalmologyRE1-994ENInternational Journal of Ophthalmology, Vol 14, Iss 12, Pp 1828-1833 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human umbilical cord mesenchymal stem cells exosomes diabetic retinopathy fundus fluorescein angiography optical coherence tomography rat Ophthalmology RE1-994
spellingShingle	human umbilical cord mesenchymal stem cells exosomes diabetic retinopathy fundus fluorescein angiography optical coherence tomography rat Ophthalmology RE1-994 Yan Fu Xiang Gao Guang-Hui He Song Chen Zhao-Hui Gu Yue-Ling Zhang Li-Ying Li Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
description	AIM: To assess the protective effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exs) in a diabetic rat model by using a variety of retinal bioassays. METHODS: hucMSCs were subjected to differential ultracentrifugation for the collection of exosomes, and transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) using a NanoSight analysis system and Western blotting (WB) were used to analyze the expression of surface marker proteins such as CD63, CD9 and Calnexin. Streptozotocin (STZ) was injected into the intraperitoneal cavity to establish a diabetic model. Rats were divided into a normal group, diabetic group and hucMSC-Ex group. Fundus fluorescein angiography (FFA), optical coherence tomography (OCT) and other live imaging methods were used to observe the fundus of the rats. Finally, the eyeballs of rats from each group were collected for hematoxylin-eosin (HE) staining to further analyze the retinal structure. RESULTS: Through TEM, NTA and WB, we successfully isolated hucMSC-Exs. Subsequent FFA and OCT confirmed that hucMSC-Exs effectively prevented early retinal vascular damage and thickening of the retina. Finally, HE staining of rat retinal sections revealed that exosomes effectively alleviated retinal structure disruption caused by diabetes. CONCLUSION: hucMSC-Exs have a protective effect on the retina in diabetic rat through FFA, OCT and HE staining.
format	article
author	Yan Fu Xiang Gao Guang-Hui He Song Chen Zhao-Hui Gu Yue-Ling Zhang Li-Ying Li
author_facet	Yan Fu Xiang Gao Guang-Hui He Song Chen Zhao-Hui Gu Yue-Ling Zhang Li-Ying Li
author_sort	Yan Fu
title	Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
title_short	Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
title_full	Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
title_fullStr	Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
title_full_unstemmed	Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
title_sort	protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging
publisher	Press of International Journal of Ophthalmology (IJO PRESS)
publishDate	2021
url	https://doaj.org/article/124e15ac92f642bd8ebd4dc6fcd37a85
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_version_	1718409884474540032

Protective effects of umbilical cord mesenchymal stem cell exosomes in a diabetic rat model through live retinal imaging

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