Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals

Objective: The aim of this study was to evaluate the effects of individual or combined use of two antioxidants, melatonin and famotidine on radiation induced apoptosis in leukocytes from breast cancer (BC) patients. Materials and Methods: In this experimental study, the DPPH assay was used to deter...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elham Samei, Hossein Mozdarani, Farhad Samiei, Gholamreza Javadi
Formato:	article
Lenguaje:	EN
Publicado:	Royan Institute (ACECR), Tehran 2021
Materias:	antioxidants apoptosis breast cancer ionizing radiation leukocytes Medicine R Science Q
Acceso en línea:	https://doaj.org/article/124ee3dc00c74f68aa295b44188508aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:124ee3dc00c74f68aa295b44188508aa
record_format	dspace
spelling	oai:doaj.org-article:124ee3dc00c74f68aa295b44188508aa2021-11-07T07:54:53ZRadioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals10.22074/cellj.2021.73782228-58062228-5814https://doaj.org/article/124ee3dc00c74f68aa295b44188508aa2021-10-01T00:00:00Zhttps://celljournal.org/journal/article/fulltext/modulating-effect-of-combined-melatonin-and-famotidine-treatment-on-radiation-induced-apoptosis-in-peripheral-blood-leukocytes-of-breast-cancer-patients-and-normal-individuals.pdfhttps://doaj.org/toc/2228-5806https://doaj.org/toc/2228-5814Objective: The aim of this study was to evaluate the effects of individual or combined use of two antioxidants, melatonin and famotidine on radiation induced apoptosis in leukocytes from breast cancer (BC) patients. Materials and Methods: In this experimental study, the DPPH assay was used to determine the appropriate doses of melatonin and famotidine for treatment of BC and control leukocytes. The leukocytes were cultured in complete RPMI- 1640 medium and treated with either agent for two hours. Cells were exposed to 4 Gy gamma rays generated from a Co-60 source at a dose rate of 0.85 Gy for 48 hours before harvesting. The cells were placed on slides and the neutral comet assay was performed. A total of 500 cells were stained with ethidium bromide and assessed for the amount of apoptosis under a fluorescent microscope x400 magnification. Results: We observed significantly more apoptosis following radiation alone in the leukocytes from BC patients compared with normal individuals (P<0.01). Individual use of famotidine and melatonin induced very low frequencies of apoptosis that was not significantly different from the control (P>0.05). However, when combined with radiation, there was a decreased frequency of apoptosis in leukocytes of both normal and BC patients (P<0.05). The effect of famotidine was more pronounced than melatonin. Conclusion: Melatonin, despite its potent antioxidant property, does not significantly affect radiation induced apoptosis in leukocytes derived from normal individuals; however, it has a moderately significant protective effect on in leukocytes derived from BC patients. Therefore, when used with radiation it might not intervene with the radiotherapy (RT) regimen of BC cancer patients. Famotidine is a good radioprotector for normal tissue. However, the efficacy of RT might be reduced with an accumulation of famotidine in tumour tissues.Elham SameiHossein MozdaraniFarhad SamieiGholamreza JavadiRoyan Institute (ACECR), Tehranarticleantioxidantsapoptosisbreast cancerionizing radiationleukocytesMedicineRScienceQENCell Journal, Vol 23, Iss 5, Pp 562-567 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidants apoptosis breast cancer ionizing radiation leukocytes Medicine R Science Q
spellingShingle	antioxidants apoptosis breast cancer ionizing radiation leukocytes Medicine R Science Q Elham Samei Hossein Mozdarani Farhad Samiei Gholamreza Javadi Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals
description	Objective: The aim of this study was to evaluate the effects of individual or combined use of two antioxidants, melatonin and famotidine on radiation induced apoptosis in leukocytes from breast cancer (BC) patients. Materials and Methods: In this experimental study, the DPPH assay was used to determine the appropriate doses of melatonin and famotidine for treatment of BC and control leukocytes. The leukocytes were cultured in complete RPMI- 1640 medium and treated with either agent for two hours. Cells were exposed to 4 Gy gamma rays generated from a Co-60 source at a dose rate of 0.85 Gy for 48 hours before harvesting. The cells were placed on slides and the neutral comet assay was performed. A total of 500 cells were stained with ethidium bromide and assessed for the amount of apoptosis under a fluorescent microscope x400 magnification. Results: We observed significantly more apoptosis following radiation alone in the leukocytes from BC patients compared with normal individuals (P<0.01). Individual use of famotidine and melatonin induced very low frequencies of apoptosis that was not significantly different from the control (P>0.05). However, when combined with radiation, there was a decreased frequency of apoptosis in leukocytes of both normal and BC patients (P<0.05). The effect of famotidine was more pronounced than melatonin. Conclusion: Melatonin, despite its potent antioxidant property, does not significantly affect radiation induced apoptosis in leukocytes derived from normal individuals; however, it has a moderately significant protective effect on in leukocytes derived from BC patients. Therefore, when used with radiation it might not intervene with the radiotherapy (RT) regimen of BC cancer patients. Famotidine is a good radioprotector for normal tissue. However, the efficacy of RT might be reduced with an accumulation of famotidine in tumour tissues.
format	article
author	Elham Samei Hossein Mozdarani Farhad Samiei Gholamreza Javadi
author_facet	Elham Samei Hossein Mozdarani Farhad Samiei Gholamreza Javadi
author_sort	Elham Samei
title	Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals
title_short	Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals
title_full	Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals
title_fullStr	Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals
title_full_unstemmed	Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals
title_sort	radioprotective effects of combined melatonin and famotidine treatment on radiation induced apoptosis in peripheral blood leukocytes of breast cancer patients and normal individuals
publisher	Royan Institute (ACECR), Tehran
publishDate	2021
url	https://doaj.org/article/124ee3dc00c74f68aa295b44188508aa
work_keys_str_mv	AT elhamsamei radioprotectiveeffectsofcombinedmelatoninandfamotidinetreatmentonradiationinducedapoptosisinperipheralbloodleukocytesofbreastcancerpatientsandnormalindividuals AT hosseinmozdarani radioprotectiveeffectsofcombinedmelatoninandfamotidinetreatmentonradiationinducedapoptosisinperipheralbloodleukocytesofbreastcancerpatientsandnormalindividuals AT farhadsamiei radioprotectiveeffectsofcombinedmelatoninandfamotidinetreatmentonradiationinducedapoptosisinperipheralbloodleukocytesofbreastcancerpatientsandnormalindividuals AT gholamrezajavadi radioprotectiveeffectsofcombinedmelatoninandfamotidinetreatmentonradiationinducedapoptosisinperipheralbloodleukocytesofbreastcancerpatientsandnormalindividuals
_version_	1718443530188226560

Radioprotective Effects of Combined Melatonin and Famotidine Treatment on Radiation Induced Apoptosis in Peripheral Blood Leukocytes of Breast Cancer Patients and Normal Individuals

Ejemplares similares