EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS

Abstract. In this study, we evaluated effects of statins and other biological preparations upon spontaneous and stimulated activation of МАРК p38 and ERK1/2 in monocytes from the patients with rheumatoid arthritis (RA). We used peripheral blood mononuclear cells (PBMC) from RA patients and healthy d...

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Autores principales:	I. V. Shirinsky, V. A. Kozlov, V. S. Shirinsky
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	rheumatoid arthritis mitogen-activated proteinkinases statins biologicals Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/125341fdbf2044dfa262ffa6d2efc608
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spelling	oai:doaj.org-article:125341fdbf2044dfa262ffa6d2efc6082021-11-18T08:03:38ZEFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS1563-06252313-741X10.15789/1563-0625-2009-1-71-78https://doaj.org/article/125341fdbf2044dfa262ffa6d2efc6082014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/190https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. In this study, we evaluated effects of statins and other biological preparations upon spontaneous and stimulated activation of МАРК p38 and ERK1/2 in monocytes from the patients with rheumatoid arthritis (RA). We used peripheral blood mononuclear cells (PBMC) from RA patients and healthy donors. PBMC were cultured in presence of 0, 0.1, 1 or 10 мM mevastatin, 10 мg/ml IL-1 receptor antagonist (IL-1Ra), 5 мg/ml infliximab, and 5 мg/ml soluble pegylated p55 TNF-receptor (r-met-Hu-sTNF-RI). To study the mechanisms of mevastatin effects upon МАРК p38 and ERK1/2 activities, L-mevalonate was added to the cultures. The cells were stained with anti-phospho-MAPK p38, or anti-phospho-ERK1/2, and analyzed with flow cytometry. We have shown that IL-1Ra and r-met-Hu-sTNF-RI inhibited spontaneous MAPK р38 activation. Mevastatin reduced spontaneous MAPK p38 and ERK1/2 phosphorylation. Mevastatininduced suppression of MAPK p38 and ERK1/2 activation was not dose-dependent. L-mevalonate completely prevented mevastatin-induced reduction of MAPK р38 phosphorylation and partially reversed inhibition of МАРК ERK1/2. In conclusion, decrease in MAPK activation represents a common mechanism of anti-inflammatory effects exerted by statins and some other biologicals.I. V. ShirinskyV. A. KozlovV. S. ShirinskySPb RAACIarticlerheumatoid arthritismitogen-activated proteinkinasesstatinsbiologicalsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 1, Pp 71-78 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	rheumatoid arthritis mitogen-activated proteinkinases statins biologicals Immunologic diseases. Allergy RC581-607
spellingShingle	rheumatoid arthritis mitogen-activated proteinkinases statins biologicals Immunologic diseases. Allergy RC581-607 I. V. Shirinsky V. A. Kozlov V. S. Shirinsky EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS
description	Abstract. In this study, we evaluated effects of statins and other biological preparations upon spontaneous and stimulated activation of МАРК p38 and ERK1/2 in monocytes from the patients with rheumatoid arthritis (RA). We used peripheral blood mononuclear cells (PBMC) from RA patients and healthy donors. PBMC were cultured in presence of 0, 0.1, 1 or 10 мM mevastatin, 10 мg/ml IL-1 receptor antagonist (IL-1Ra), 5 мg/ml infliximab, and 5 мg/ml soluble pegylated p55 TNF-receptor (r-met-Hu-sTNF-RI). To study the mechanisms of mevastatin effects upon МАРК p38 and ERK1/2 activities, L-mevalonate was added to the cultures. The cells were stained with anti-phospho-MAPK p38, or anti-phospho-ERK1/2, and analyzed with flow cytometry. We have shown that IL-1Ra and r-met-Hu-sTNF-RI inhibited spontaneous MAPK р38 activation. Mevastatin reduced spontaneous MAPK p38 and ERK1/2 phosphorylation. Mevastatininduced suppression of MAPK p38 and ERK1/2 activation was not dose-dependent. L-mevalonate completely prevented mevastatin-induced reduction of MAPK р38 phosphorylation and partially reversed inhibition of МАРК ERK1/2. In conclusion, decrease in MAPK activation represents a common mechanism of anti-inflammatory effects exerted by statins and some other biologicals.
format	article
author	I. V. Shirinsky V. A. Kozlov V. S. Shirinsky
author_facet	I. V. Shirinsky V. A. Kozlov V. S. Shirinsky
author_sort	I. V. Shirinsky
title	EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS
title_short	EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS
title_full	EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS
title_fullStr	EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS
title_full_unstemmed	EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS
title_sort	effects of statins and other biological preparations upon activation of mitogen-activated protein kinases in patients with rheumatoid arthritis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/125341fdbf2044dfa262ffa6d2efc608
work_keys_str_mv	AT ivshirinsky effectsofstatinsandotherbiologicalpreparationsuponactivationofmitogenactivatedproteinkinasesinpatientswithrheumatoidarthritis AT vakozlov effectsofstatinsandotherbiologicalpreparationsuponactivationofmitogenactivatedproteinkinasesinpatientswithrheumatoidarthritis AT vsshirinsky effectsofstatinsandotherbiologicalpreparationsuponactivationofmitogenactivatedproteinkinasesinpatientswithrheumatoidarthritis
_version_	1718422526181244928

EFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITIS

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