Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells

Background: Pinus roxburghii has been used in the Himalayan region as folkloric remedy while nothing is yet known about its immunomodulatory potential. Methods: The crude extracts of green and fallen pine needles were subjected to sequential fractionation to get partially purified fractions. Human p...

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Autores principales:	Henok Gullilat, Adesh k Saini, Reena Kumari, Gourav Chandan, Reena V Saini
Formato:	article
Lenguaje:	EN
Publicado:	Tabriz University of Medical Sciences 2021
Materias:	anticancer immunomodulation granulysin gc-ms pine needles Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/1264cb9e9f52412e88ec74e6a24a14d2
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spelling	oai:doaj.org-article:1264cb9e9f52412e88ec74e6a24a14d22021-11-29T05:53:19ZPinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells2383-288610.34172/PS.2021.7https://doaj.org/article/1264cb9e9f52412e88ec74e6a24a14d22021-12-01T00:00:00Zhttps://ps.tbzmed.ac.ir/PDF/ps-27-543.pdfhttps://doaj.org/toc/2383-2886Background: Pinus roxburghii has been used in the Himalayan region as folkloric remedy while nothing is yet known about its immunomodulatory potential. Methods: The crude extracts of green and fallen pine needles were subjected to sequential fractionation to get partially purified fractions. Human peripheral blood lymphocytes (PBL)were used to analyze immunoenhancing potential of these fractions. Microcytotoxicity assay was employed to investigate improved cancer cells killing capabilities of PBL against various cancer cell lines. GC-MS was carried out to identify the major compounds in the bioactive fractions. Results: The lymphocyte proliferation assay depicted the immunoenhancing potential of extractsand fractions of fallen and green needles of P. roxburghii. The ethyl acetate fractions of both fallen and green needles displayed highest mitogenic activity on human PBL. Both fractions heightened the expression of cell surface markers (CD3, CD8, and CD56) and significantly increased the production of cytokines (IL-2, IFN-γ and TNF-α). The enhanced intracellular granulysin (immunomarker for activated CTLs and NK cells) expression also confirmed immune stimulatory potential of these fractions on human lymphocytes. The ethyl acetate fractions of pine needles enhanced the cytotoxicity of PBL towards various cancer cells (HCT-116, HeLa, PC-3 and A549) as compared to untreated PBL. GC-MS analysis showed presence of major compounds like 3-α-mannobiose, octakis (trimethylsilyl) ether, methyloxime in ethyl acetate fraction of the green needles and cyclodecasiloxane, eicosamethyl in ethyl acetate fraction of the fallen needles. Conclusion: The bioactive fractions of the fallen and green needles of P. roxburghii stimulate cancer cells killing potential of human lymphocytes.Henok GullilatAdesh k SainiReena KumariGourav ChandanReena V SainiTabriz University of Medical Sciencesarticleanticancerimmunomodulationgranulysingc-mspine needlesPharmacy and materia medicaRS1-441ENPharmaceutical Sciences, Vol 27, Iss 4, Pp 543-551 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anticancer immunomodulation granulysin gc-ms pine needles Pharmacy and materia medica RS1-441
spellingShingle	anticancer immunomodulation granulysin gc-ms pine needles Pharmacy and materia medica RS1-441 Henok Gullilat Adesh k Saini Reena Kumari Gourav Chandan Reena V Saini Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells
description	Background: Pinus roxburghii has been used in the Himalayan region as folkloric remedy while nothing is yet known about its immunomodulatory potential. Methods: The crude extracts of green and fallen pine needles were subjected to sequential fractionation to get partially purified fractions. Human peripheral blood lymphocytes (PBL)were used to analyze immunoenhancing potential of these fractions. Microcytotoxicity assay was employed to investigate improved cancer cells killing capabilities of PBL against various cancer cell lines. GC-MS was carried out to identify the major compounds in the bioactive fractions. Results: The lymphocyte proliferation assay depicted the immunoenhancing potential of extractsand fractions of fallen and green needles of P. roxburghii. The ethyl acetate fractions of both fallen and green needles displayed highest mitogenic activity on human PBL. Both fractions heightened the expression of cell surface markers (CD3, CD8, and CD56) and significantly increased the production of cytokines (IL-2, IFN-γ and TNF-α). The enhanced intracellular granulysin (immunomarker for activated CTLs and NK cells) expression also confirmed immune stimulatory potential of these fractions on human lymphocytes. The ethyl acetate fractions of pine needles enhanced the cytotoxicity of PBL towards various cancer cells (HCT-116, HeLa, PC-3 and A549) as compared to untreated PBL. GC-MS analysis showed presence of major compounds like 3-α-mannobiose, octakis (trimethylsilyl) ether, methyloxime in ethyl acetate fraction of the green needles and cyclodecasiloxane, eicosamethyl in ethyl acetate fraction of the fallen needles. Conclusion: The bioactive fractions of the fallen and green needles of P. roxburghii stimulate cancer cells killing potential of human lymphocytes.
format	article
author	Henok Gullilat Adesh k Saini Reena Kumari Gourav Chandan Reena V Saini
author_facet	Henok Gullilat Adesh k Saini Reena Kumari Gourav Chandan Reena V Saini
author_sort	Henok Gullilat
title	Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells
title_short	Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells
title_full	Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells
title_fullStr	Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells
title_full_unstemmed	Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells
title_sort	pinus roxburghii constituents augment human lymphocytes mediated cytotoxicity towards cancer cells
publisher	Tabriz University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/1264cb9e9f52412e88ec74e6a24a14d2
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_version_	1718407569433690112

Pinus roxburghii Constituents Augment Human Lymphocytes Mediated Cytotoxicity Towards Cancer Cells

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