Prostate stromal cells express the progesterone receptor to control cancer cell mobility.

<h4>Background</h4>Reciprocal interactions between epithelium and stroma play vital roles for prostate cancer development and progression. Enhanced secretions of cytokines and growth factors by cancer associated fibroblasts in prostate tumors create a favorable microenvironment for cance...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yue Yu, Jennifer Suehyun Lee, Ning Xie, Estelle Li, Antonio Hurtado-Coll, Ladan Fazli, Michael Cox, Stephen Plymate, Martin Gleave, Xuesen Dong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12681f314f5445559eabc019ebbb6400
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:12681f314f5445559eabc019ebbb6400
record_format	dspace
spelling	oai:doaj.org-article:12681f314f5445559eabc019ebbb64002021-11-18T08:26:25ZProstate stromal cells express the progesterone receptor to control cancer cell mobility.1932-620310.1371/journal.pone.0092714https://doaj.org/article/12681f314f5445559eabc019ebbb64002014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24664419/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Reciprocal interactions between epithelium and stroma play vital roles for prostate cancer development and progression. Enhanced secretions of cytokines and growth factors by cancer associated fibroblasts in prostate tumors create a favorable microenvironment for cancer cells to grow and metastasize. Our previous work showed that the progesterone receptor (PR) was expressed specifically in prostate stromal fibroblasts and smooth muscle cells. However, the expression levels of PR and its impact to tumor microenvironment in prostate tumors are poorly understood.<h4>Methods</h4>Immunohistochemistry assays are applied to human prostate tissue biopsies. Cell migration, invasion and proliferation assays are performed using human prostate cells. Real-time PCR and ELISA are applied to measure gene expression at molecular levels.<h4>Results</h4>Immunohistochemistry assays showed that PR protein levels were decreased in cancer associated stroma when compared with paired normal prostate stroma. Using in vitro prostate stromal cell models, we showed that conditioned media collected from PR positive stromal cells inhibited prostate cancer cell migration and invasion, but had minor suppressive impacts on cancer cell proliferation. PR suppressed the secretion of stromal derived factor-1 (SDF-1) and interlukin-6 (IL-6) by stromal cells independent to PR ligands. Blocking PR expression by siRNA or supplementation of exogenous SDF-1 or IL-6 to conditioned media from PR positive stromal cells counteracted the inhibitory effects of PR to cancer cell migration and invasion.<h4>Conclusions</h4>Decreased expression of the PR in cancer associated stroma may contribute to the elevated SDF-1 and IL-6 levels in prostate tumors and enhance prostate tumor progression.Yue YuJennifer Suehyun LeeNing XieEstelle LiAntonio Hurtado-CollLadan FazliMichael CoxStephen PlymateMartin GleaveXuesen DongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92714 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yue Yu Jennifer Suehyun Lee Ning Xie Estelle Li Antonio Hurtado-Coll Ladan Fazli Michael Cox Stephen Plymate Martin Gleave Xuesen Dong Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
description	<h4>Background</h4>Reciprocal interactions between epithelium and stroma play vital roles for prostate cancer development and progression. Enhanced secretions of cytokines and growth factors by cancer associated fibroblasts in prostate tumors create a favorable microenvironment for cancer cells to grow and metastasize. Our previous work showed that the progesterone receptor (PR) was expressed specifically in prostate stromal fibroblasts and smooth muscle cells. However, the expression levels of PR and its impact to tumor microenvironment in prostate tumors are poorly understood.<h4>Methods</h4>Immunohistochemistry assays are applied to human prostate tissue biopsies. Cell migration, invasion and proliferation assays are performed using human prostate cells. Real-time PCR and ELISA are applied to measure gene expression at molecular levels.<h4>Results</h4>Immunohistochemistry assays showed that PR protein levels were decreased in cancer associated stroma when compared with paired normal prostate stroma. Using in vitro prostate stromal cell models, we showed that conditioned media collected from PR positive stromal cells inhibited prostate cancer cell migration and invasion, but had minor suppressive impacts on cancer cell proliferation. PR suppressed the secretion of stromal derived factor-1 (SDF-1) and interlukin-6 (IL-6) by stromal cells independent to PR ligands. Blocking PR expression by siRNA or supplementation of exogenous SDF-1 or IL-6 to conditioned media from PR positive stromal cells counteracted the inhibitory effects of PR to cancer cell migration and invasion.<h4>Conclusions</h4>Decreased expression of the PR in cancer associated stroma may contribute to the elevated SDF-1 and IL-6 levels in prostate tumors and enhance prostate tumor progression.
format	article
author	Yue Yu Jennifer Suehyun Lee Ning Xie Estelle Li Antonio Hurtado-Coll Ladan Fazli Michael Cox Stephen Plymate Martin Gleave Xuesen Dong
author_facet	Yue Yu Jennifer Suehyun Lee Ning Xie Estelle Li Antonio Hurtado-Coll Ladan Fazli Michael Cox Stephen Plymate Martin Gleave Xuesen Dong
author_sort	Yue Yu
title	Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
title_short	Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
title_full	Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
title_fullStr	Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
title_full_unstemmed	Prostate stromal cells express the progesterone receptor to control cancer cell mobility.
title_sort	prostate stromal cells express the progesterone receptor to control cancer cell mobility.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/12681f314f5445559eabc019ebbb6400
work_keys_str_mv	AT yueyu prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT jennifersuehyunlee prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT ningxie prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT estelleli prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT antoniohurtadocoll prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT ladanfazli prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT michaelcox prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT stephenplymate prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT martingleave prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility AT xuesendong prostatestromalcellsexpresstheprogesteronereceptortocontrolcancercellmobility
_version_	1718421786153975808

Prostate stromal cells express the progesterone receptor to control cancer cell mobility.

Ejemplares similares