Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different re...

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Autores principales: Guillaume Tetreau, Anne-Sophie Banneville, Elena A. Andreeva, Aaron S. Brewster, Mark S. Hunter, Raymond G. Sierra, Jean-Marie Teulon, Iris D. Young, Niamh Burke, Tilman A. Grünewald, Joël Beaudouin, Irina Snigireva, Maria Teresa Fernandez-Luna, Alister Burt, Hyun-Woo Park, Luca Signor, Jayesh A. Bafna, Rabia Sadir, Daphna Fenel, Elisabetta Boeri-Erba, Maria Bacia, Ninon Zala, Frédéric Laporte, Laurence Després, Martin Weik, Sébastien Boutet, Martin Rosenthal, Nicolas Coquelle, Manfred Burghammer, Duilio Cascio, Michael R. Sawaya, Mathias Winterhalter, Enrico Gratton, Irina Gutsche, Brian Federici, Jean-Luc Pellequer, Nicholas K. Sauter, Jacques-Philippe Colletier
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Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/126ca4694d8a47efbec919f942778d20
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spelling oai:doaj.org-article:126ca4694d8a47efbec919f942778d202021-12-02T14:41:13ZSerial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade10.1038/s41467-020-14894-w2041-1723https://doaj.org/article/126ca4694d8a47efbec919f942778d202020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14894-whttps://doaj.org/toc/2041-1723Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different redox and pH conditions and by combining their structural data with further biochemical, toxicological and biophysical analyses provide mechanistic insights into the Cyt1Aa bioactivation cascade.Guillaume TetreauAnne-Sophie BannevilleElena A. AndreevaAaron S. BrewsterMark S. HunterRaymond G. SierraJean-Marie TeulonIris D. YoungNiamh BurkeTilman A. GrünewaldJoël BeaudouinIrina SnigirevaMaria Teresa Fernandez-LunaAlister BurtHyun-Woo ParkLuca SignorJayesh A. BafnaRabia SadirDaphna FenelElisabetta Boeri-ErbaMaria BaciaNinon ZalaFrédéric LaporteLaurence DesprésMartin WeikSébastien BoutetMartin RosenthalNicolas CoquelleManfred BurghammerDuilio CascioMichael R. SawayaMathias WinterhalterEnrico GrattonIrina GutscheBrian FedericiJean-Luc PellequerNicholas K. SauterJacques-Philippe ColletierNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-16 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Guillaume Tetreau
Anne-Sophie Banneville
Elena A. Andreeva
Aaron S. Brewster
Mark S. Hunter
Raymond G. Sierra
Jean-Marie Teulon
Iris D. Young
Niamh Burke
Tilman A. Grünewald
Joël Beaudouin
Irina Snigireva
Maria Teresa Fernandez-Luna
Alister Burt
Hyun-Woo Park
Luca Signor
Jayesh A. Bafna
Rabia Sadir
Daphna Fenel
Elisabetta Boeri-Erba
Maria Bacia
Ninon Zala
Frédéric Laporte
Laurence Després
Martin Weik
Sébastien Boutet
Martin Rosenthal
Nicolas Coquelle
Manfred Burghammer
Duilio Cascio
Michael R. Sawaya
Mathias Winterhalter
Enrico Gratton
Irina Gutsche
Brian Federici
Jean-Luc Pellequer
Nicholas K. Sauter
Jacques-Philippe Colletier
Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
description Bacillus thuringiensis israelensis (Bti) produces the naturally-crystalline proteinaceous toxin Cyt1Aa that is toxic to mosquito larvae. Here the authors grow recombinant nanocrystals of the Cyt1Aa protoxin in vivo and use serial femtosecond crystallography to determine its structure at different redox and pH conditions and by combining their structural data with further biochemical, toxicological and biophysical analyses provide mechanistic insights into the Cyt1Aa bioactivation cascade.
format article
author Guillaume Tetreau
Anne-Sophie Banneville
Elena A. Andreeva
Aaron S. Brewster
Mark S. Hunter
Raymond G. Sierra
Jean-Marie Teulon
Iris D. Young
Niamh Burke
Tilman A. Grünewald
Joël Beaudouin
Irina Snigireva
Maria Teresa Fernandez-Luna
Alister Burt
Hyun-Woo Park
Luca Signor
Jayesh A. Bafna
Rabia Sadir
Daphna Fenel
Elisabetta Boeri-Erba
Maria Bacia
Ninon Zala
Frédéric Laporte
Laurence Després
Martin Weik
Sébastien Boutet
Martin Rosenthal
Nicolas Coquelle
Manfred Burghammer
Duilio Cascio
Michael R. Sawaya
Mathias Winterhalter
Enrico Gratton
Irina Gutsche
Brian Federici
Jean-Luc Pellequer
Nicholas K. Sauter
Jacques-Philippe Colletier
author_facet Guillaume Tetreau
Anne-Sophie Banneville
Elena A. Andreeva
Aaron S. Brewster
Mark S. Hunter
Raymond G. Sierra
Jean-Marie Teulon
Iris D. Young
Niamh Burke
Tilman A. Grünewald
Joël Beaudouin
Irina Snigireva
Maria Teresa Fernandez-Luna
Alister Burt
Hyun-Woo Park
Luca Signor
Jayesh A. Bafna
Rabia Sadir
Daphna Fenel
Elisabetta Boeri-Erba
Maria Bacia
Ninon Zala
Frédéric Laporte
Laurence Després
Martin Weik
Sébastien Boutet
Martin Rosenthal
Nicolas Coquelle
Manfred Burghammer
Duilio Cascio
Michael R. Sawaya
Mathias Winterhalter
Enrico Gratton
Irina Gutsche
Brian Federici
Jean-Luc Pellequer
Nicholas K. Sauter
Jacques-Philippe Colletier
author_sort Guillaume Tetreau
title Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
title_short Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
title_full Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
title_fullStr Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
title_full_unstemmed Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade
title_sort serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal cyt1aa bioactivation cascade
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/126ca4694d8a47efbec919f942778d20
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