Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia

Abstract. Benget VV, Retnaningrum E. 2020. Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia. Biodiversitas 21: 21-27. The increasing number of human population density around mangro...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Vivekananda Vinsensius Benget, ENDAH RETNANINGRUM
Formato: article
Lenguaje:EN
Publicado: MBI & UNS Solo 2019
Materias:
Acceso en línea:https://doaj.org/article/12755610fbc9448ab727d16b437ddeb8
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:12755610fbc9448ab727d16b437ddeb8
record_format dspace
spelling oai:doaj.org-article:12755610fbc9448ab727d16b437ddeb82021-11-21T22:09:25ZActivities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia1412-033X2085-472210.13057/biodiv/d210104https://doaj.org/article/12755610fbc9448ab727d16b437ddeb82019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3947https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Benget VV, Retnaningrum E. 2020. Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia. Biodiversitas 21: 21-27. The increasing number of human population density around mangrove ecosystems has resulted in its pollution, particularly oil spills. Rhizobacteria have metabolic abilities that make them possible to live in polluted environmental conditions and the potential to be biological agents for oil remediation. However, investigations of rhizobacteria especially from rhizospheres of mangrove plants (Rhizophora sp.) and their potential use in remediation remains limited. Therefore, the purposes of this research were to investigate the ability of selected isolate in the degradation of petroleum hydrocarbons and identify its isolate using 16S rRNA gene. Observation of that degradation ability was conducted by inoculating the isolate in Bushnell-Haas Minimal Salt (BHMS) medium containing crude petroleum 0.01 %. After 10 days of incubation, the degradation hydrocarbons were analyzed by measuring Total Petroleum Hydrocarbons (TPH) using Gas Chromatography-Flame Ionization Detector (GC-FID) method. Four rhizobacteria isolate from rhizospheres of mangrove plants (Rhizophora sp), NMKP1, NMKP2, NMKP3, and NMKP4 were analyzed based on their growth in Bushnell-Haas Minimal Salt (BHMS) medium containing crude petroleum 0.01 %. The results revealed that NMKP4 isolate had the highest specific growth rate. In addition, it could degrade those hydrocarbons pollutants as much of 98.72 % within 10 days. Based on molecular characterization showed that NMKP4 isolate was in the same cluster as Pseudomonas sp. CO03.Vivekananda Vinsensius BengetENDAH RETNANINGRUMMBI & UNS Soloarticle16s rrna, gc-fid, mangroves, oil spills, pseudomonas, rhizophoraBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution DOAJ
collection DOAJ
language EN
topic 16s rrna, gc-fid, mangroves, oil spills, pseudomonas, rhizophora
Biology (General)
QH301-705.5
spellingShingle 16s rrna, gc-fid, mangroves, oil spills, pseudomonas, rhizophora
Biology (General)
QH301-705.5
Vivekananda Vinsensius Benget
ENDAH RETNANINGRUM
Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
description Abstract. Benget VV, Retnaningrum E. 2020. Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia. Biodiversitas 21: 21-27. The increasing number of human population density around mangrove ecosystems has resulted in its pollution, particularly oil spills. Rhizobacteria have metabolic abilities that make them possible to live in polluted environmental conditions and the potential to be biological agents for oil remediation. However, investigations of rhizobacteria especially from rhizospheres of mangrove plants (Rhizophora sp.) and their potential use in remediation remains limited. Therefore, the purposes of this research were to investigate the ability of selected isolate in the degradation of petroleum hydrocarbons and identify its isolate using 16S rRNA gene. Observation of that degradation ability was conducted by inoculating the isolate in Bushnell-Haas Minimal Salt (BHMS) medium containing crude petroleum 0.01 %. After 10 days of incubation, the degradation hydrocarbons were analyzed by measuring Total Petroleum Hydrocarbons (TPH) using Gas Chromatography-Flame Ionization Detector (GC-FID) method. Four rhizobacteria isolate from rhizospheres of mangrove plants (Rhizophora sp), NMKP1, NMKP2, NMKP3, and NMKP4 were analyzed based on their growth in Bushnell-Haas Minimal Salt (BHMS) medium containing crude petroleum 0.01 %. The results revealed that NMKP4 isolate had the highest specific growth rate. In addition, it could degrade those hydrocarbons pollutants as much of 98.72 % within 10 days. Based on molecular characterization showed that NMKP4 isolate was in the same cluster as Pseudomonas sp. CO03.
format article
author Vivekananda Vinsensius Benget
ENDAH RETNANINGRUM
author_facet Vivekananda Vinsensius Benget
ENDAH RETNANINGRUM
author_sort Vivekananda Vinsensius Benget
title Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
title_short Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
title_full Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
title_fullStr Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
title_full_unstemmed Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
title_sort activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (rhizophora sp.) in kulon progo, yogyakarta, indonesia
publisher MBI & UNS Solo
publishDate 2019
url https://doaj.org/article/12755610fbc9448ab727d16b437ddeb8
work_keys_str_mv AT vivekanandavinsensiusbenget activitiesandmolecularcharacterizationofpetroleumhydrocarbonsdegradingrhizobacteriafrommangroveplantsrhizophoraspinkulonprogoyogyakartaindonesia
AT endahretnaningrum activitiesandmolecularcharacterizationofpetroleumhydrocarbonsdegradingrhizobacteriafrommangroveplantsrhizophoraspinkulonprogoyogyakartaindonesia
_version_ 1718418565805113344