Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.

Some bacterial strains of the multidrug-resistant Gram-positive bacteria Enterococcus faecalis can significantly reduce the efficacy of conventional antimicrobial chemotherapy. Thus, the introduction of bacteriophage (phage) therapy is expected, where a phage is used as a bioagent to destroy bacteri...

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Autores principales:	Jumpei Uchiyama, Iyo Takemura, Miho Satoh, Shin-ichiro Kato, Takako Ujihara, Kazue Akechi, Shigenobu Matsuzaki, Masanori Daibata
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/127acb0953624d7ab40103c861fea54d
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spelling	oai:doaj.org-article:127acb0953624d7ab40103c861fea54d2021-11-18T07:35:48ZImproved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.1932-620310.1371/journal.pone.0026648https://doaj.org/article/127acb0953624d7ab40103c861fea54d2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046321/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Some bacterial strains of the multidrug-resistant Gram-positive bacteria Enterococcus faecalis can significantly reduce the efficacy of conventional antimicrobial chemotherapy. Thus, the introduction of bacteriophage (phage) therapy is expected, where a phage is used as a bioagent to destroy bacteria. E. faecalis phage ΦEF24C is known to be a good candidate for a therapeutic phage against E. faecalis. However, this therapeutic phage still produces nonuniform antimicrobial effects with different bacterial strains of the same species and this might prove detrimental to its therapeutic effects. One solution to this problem is the preparation of mutant phages with higher activity, based on a scientific rationale. This study isolated and analyzed a spontaneous mutant phage, ΦEF24C-P2, which exhibited higher infectivity against various bacterial strains when compared with phage ΦEF24C. First, the improved bactericidal effects of phage ΦEF24C-P2 were attributable to its increased adsorption rate. Moreover, genomic sequence scanning revealed that phage ΦEF24C-P2 had a point mutation in orf31. Proteomic analysis showed that ORF31 (mw, 203 kDa) was present in structural components, and immunological analysis using rabbit-derived antibodies showed that it was a component of a long, flexible fine tail fiber extending from the tail end. Finally, phage ΦEF24C-P2 also showed higher bactericidal activity in human blood compared with phage ΦEF24C using the in vitro assay system. In conclusion, the therapeutic effects of phage ΦEF24C-P2 were improved by a point mutation in gene orf31, which encoded a tail fiber component.Jumpei UchiyamaIyo TakemuraMiho SatohShin-ichiro KatoTakako UjiharaKazue AkechiShigenobu MatsuzakiMasanori DaibataPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26648 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jumpei Uchiyama Iyo Takemura Miho Satoh Shin-ichiro Kato Takako Ujihara Kazue Akechi Shigenobu Matsuzaki Masanori Daibata Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
description	Some bacterial strains of the multidrug-resistant Gram-positive bacteria Enterococcus faecalis can significantly reduce the efficacy of conventional antimicrobial chemotherapy. Thus, the introduction of bacteriophage (phage) therapy is expected, where a phage is used as a bioagent to destroy bacteria. E. faecalis phage ΦEF24C is known to be a good candidate for a therapeutic phage against E. faecalis. However, this therapeutic phage still produces nonuniform antimicrobial effects with different bacterial strains of the same species and this might prove detrimental to its therapeutic effects. One solution to this problem is the preparation of mutant phages with higher activity, based on a scientific rationale. This study isolated and analyzed a spontaneous mutant phage, ΦEF24C-P2, which exhibited higher infectivity against various bacterial strains when compared with phage ΦEF24C. First, the improved bactericidal effects of phage ΦEF24C-P2 were attributable to its increased adsorption rate. Moreover, genomic sequence scanning revealed that phage ΦEF24C-P2 had a point mutation in orf31. Proteomic analysis showed that ORF31 (mw, 203 kDa) was present in structural components, and immunological analysis using rabbit-derived antibodies showed that it was a component of a long, flexible fine tail fiber extending from the tail end. Finally, phage ΦEF24C-P2 also showed higher bactericidal activity in human blood compared with phage ΦEF24C using the in vitro assay system. In conclusion, the therapeutic effects of phage ΦEF24C-P2 were improved by a point mutation in gene orf31, which encoded a tail fiber component.
format	article
author	Jumpei Uchiyama Iyo Takemura Miho Satoh Shin-ichiro Kato Takako Ujihara Kazue Akechi Shigenobu Matsuzaki Masanori Daibata
author_facet	Jumpei Uchiyama Iyo Takemura Miho Satoh Shin-ichiro Kato Takako Ujihara Kazue Akechi Shigenobu Matsuzaki Masanori Daibata
author_sort	Jumpei Uchiyama
title	Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
title_short	Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
title_full	Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
title_fullStr	Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
title_full_unstemmed	Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.
title_sort	improved adsorption of an enterococcus faecalis bacteriophage φef24c with a spontaneous point mutation.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/127acb0953624d7ab40103c861fea54d
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Improved adsorption of an Enterococcus faecalis bacteriophage ΦEF24C with a spontaneous point mutation.

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