Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.

Transgenic crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce the benefits of this approach. Recessive mutations in the extracellular domain of toxin-binding cadherin proteins that confer resistance...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Haonan Zhang, Shuwen Wu, Yihua Yang, Bruce E Tabashnik, Yidong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/127ebc458273433db91615b6c71e5579
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:127ebc458273433db91615b6c71e5579
record_format	dspace
spelling	oai:doaj.org-article:127ebc458273433db91615b6c71e55792021-11-18T08:03:11ZNon-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.1932-620310.1371/journal.pone.0053418https://doaj.org/article/127ebc458273433db91615b6c71e55792012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285292/?tool=EBIhttps://doaj.org/toc/1932-6203Transgenic crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce the benefits of this approach. Recessive mutations in the extracellular domain of toxin-binding cadherin proteins that confer resistance to Bt toxin Cry1Ac by disrupting toxin binding have been reported previously in three major lepidopteran pests, including the cotton bollworm, Helicoverpa armigera. Here we report a novel allele from cotton bollworm with a deletion in the intracellular domain of cadherin that is genetically linked with non-recessive resistance to Cry1Ac. We discovered this allele in each of three field-selected populations we screened from northern China where Bt cotton producing Cry1Ac has been grown intensively. We expressed four types of cadherin alleles in heterologous cell cultures: susceptible, resistant with the intracellular domain mutation, and two complementary chimeric alleles with and without the mutation. Cells transfected with each of the four cadherin alleles bound Cry1Ac and were killed by Cry1Ac. However, relative to cells transfected with either the susceptible allele or the chimeric allele lacking the intracellular domain mutation, cells transfected with the resistant allele or the chimeric allele containing the intracellular domain mutation were less susceptible to Cry1Ac. These results suggest that the intracellular domain of cadherin is involved in post-binding events that affect toxicity of Cry1Ac. This evidence is consistent with the vital role of the intracellular region of cadherin proposed by the cell signaling model of the mode of action of Bt toxins. Considered together with previously reported data, the results suggest that both pore formation and cell signaling pathways contribute to the efficacy of Bt toxins.Haonan ZhangShuwen WuYihua YangBruce E TabashnikYidong WuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e53418 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haonan Zhang Shuwen Wu Yihua Yang Bruce E Tabashnik Yidong Wu Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
description	Transgenic crops producing Bacillus thuringiensis (Bt) toxins have been planted widely to control insect pests, yet evolution of resistance by the pests can reduce the benefits of this approach. Recessive mutations in the extracellular domain of toxin-binding cadherin proteins that confer resistance to Bt toxin Cry1Ac by disrupting toxin binding have been reported previously in three major lepidopteran pests, including the cotton bollworm, Helicoverpa armigera. Here we report a novel allele from cotton bollworm with a deletion in the intracellular domain of cadherin that is genetically linked with non-recessive resistance to Cry1Ac. We discovered this allele in each of three field-selected populations we screened from northern China where Bt cotton producing Cry1Ac has been grown intensively. We expressed four types of cadherin alleles in heterologous cell cultures: susceptible, resistant with the intracellular domain mutation, and two complementary chimeric alleles with and without the mutation. Cells transfected with each of the four cadherin alleles bound Cry1Ac and were killed by Cry1Ac. However, relative to cells transfected with either the susceptible allele or the chimeric allele lacking the intracellular domain mutation, cells transfected with the resistant allele or the chimeric allele containing the intracellular domain mutation were less susceptible to Cry1Ac. These results suggest that the intracellular domain of cadherin is involved in post-binding events that affect toxicity of Cry1Ac. This evidence is consistent with the vital role of the intracellular region of cadherin proposed by the cell signaling model of the mode of action of Bt toxins. Considered together with previously reported data, the results suggest that both pore formation and cell signaling pathways contribute to the efficacy of Bt toxins.
format	article
author	Haonan Zhang Shuwen Wu Yihua Yang Bruce E Tabashnik Yidong Wu
author_facet	Haonan Zhang Shuwen Wu Yihua Yang Bruce E Tabashnik Yidong Wu
author_sort	Haonan Zhang
title	Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
title_short	Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
title_full	Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
title_fullStr	Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
title_full_unstemmed	Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
title_sort	non-recessive bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/127ebc458273433db91615b6c71e5579
work_keys_str_mv	AT haonanzhang nonrecessivebttoxinresistanceconferredbyanintracellularcadherinmutationinfieldselectedpopulationsofcottonbollworm AT shuwenwu nonrecessivebttoxinresistanceconferredbyanintracellularcadherinmutationinfieldselectedpopulationsofcottonbollworm AT yihuayang nonrecessivebttoxinresistanceconferredbyanintracellularcadherinmutationinfieldselectedpopulationsofcottonbollworm AT bruceetabashnik nonrecessivebttoxinresistanceconferredbyanintracellularcadherinmutationinfieldselectedpopulationsofcottonbollworm AT yidongwu nonrecessivebttoxinresistanceconferredbyanintracellularcadherinmutationinfieldselectedpopulationsofcottonbollworm
_version_	1718422589470146560

Non-recessive Bt toxin resistance conferred by an intracellular cadherin mutation in field-selected populations of cotton bollworm.

Ejemplares similares