Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.

Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated va...

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Autores principales:	Roy R Chaudhuri, Sarah E Peters, Stephen J Pleasance, Helen Northen, Chrissie Willers, Gavin K Paterson, Danielle B Cone, Andrew G Allen, Paul J Owen, Gil Shalom, Dov J Stekel, Ian G Charles, Duncan J Maskell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/128c00aac92c42bd828980f4e8d62ada
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spelling	oai:doaj.org-article:128c00aac92c42bd828980f4e8d62ada2021-11-25T05:47:44ZComprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.1553-73661553-737410.1371/journal.ppat.1000529https://doaj.org/article/128c00aac92c42bd828980f4e8d62ada2009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19649318/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated vaccines. A quantitative screen, Transposon Mediated Differential Hybridisation (TMDH), has been developed that identifies those members of a large library of transposon mutants that are attenuated. TMDH employs custom transposons with outward-facing T7 and SP6 promoters. Fluorescently-labelled transcripts from the promoters are hybridised to whole-genome tiling microarrays, to allow the position of the transposon insertions to be determined. Comparison of microarray data from the mutant library grown in vitro (input) with equivalent data produced after passage of the library through mice (output) enables an attenuation score to be determined for each transposon mutant. These scores are significantly correlated with bacterial counts obtained during infection of mice using mutants with individual defined deletions of the same genes. Defined deletion mutants of several novel targets identified in the TMDH screen are effective live vaccines.Roy R ChaudhuriSarah E PetersStephen J PleasanceHelen NorthenChrissie WillersGavin K PatersonDanielle B ConeAndrew G AllenPaul J OwenGil ShalomDov J StekelIan G CharlesDuncan J MaskellPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 7, p e1000529 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Roy R Chaudhuri Sarah E Peters Stephen J Pleasance Helen Northen Chrissie Willers Gavin K Paterson Danielle B Cone Andrew G Allen Paul J Owen Gil Shalom Dov J Stekel Ian G Charles Duncan J Maskell Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
description	Genes required for infection of mice by Salmonella Typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. Inactivation of such genes produces attenuated S. Typhimurium strains that have potential for use as live attenuated vaccines. A quantitative screen, Transposon Mediated Differential Hybridisation (TMDH), has been developed that identifies those members of a large library of transposon mutants that are attenuated. TMDH employs custom transposons with outward-facing T7 and SP6 promoters. Fluorescently-labelled transcripts from the promoters are hybridised to whole-genome tiling microarrays, to allow the position of the transposon insertions to be determined. Comparison of microarray data from the mutant library grown in vitro (input) with equivalent data produced after passage of the library through mice (output) enables an attenuation score to be determined for each transposon mutant. These scores are significantly correlated with bacterial counts obtained during infection of mice using mutants with individual defined deletions of the same genes. Defined deletion mutants of several novel targets identified in the TMDH screen are effective live vaccines.
format	article
author	Roy R Chaudhuri Sarah E Peters Stephen J Pleasance Helen Northen Chrissie Willers Gavin K Paterson Danielle B Cone Andrew G Allen Paul J Owen Gil Shalom Dov J Stekel Ian G Charles Duncan J Maskell
author_facet	Roy R Chaudhuri Sarah E Peters Stephen J Pleasance Helen Northen Chrissie Willers Gavin K Paterson Danielle B Cone Andrew G Allen Paul J Owen Gil Shalom Dov J Stekel Ian G Charles Duncan J Maskell
author_sort	Roy R Chaudhuri
title	Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
title_short	Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
title_full	Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
title_fullStr	Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
title_full_unstemmed	Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.
title_sort	comprehensive identification of salmonella enterica serovar typhimurium genes required for infection of balb/c mice.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/128c00aac92c42bd828980f4e8d62ada
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Comprehensive identification of Salmonella enterica serovar typhimurium genes required for infection of BALB/c mice.

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