A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration

Ping Mou,1 Haitao Peng,2 Li Zhou,3 Lin Li,2 Hong Li,2 Qiang Huang11Department of Orthopedic Surgery, West China Hospital, West China Medical School, Sichuan University; 2School of Physical Science and Technology, Sichuan University; 3Core Facility of West China Hospital, Sichuan University, Chengdu,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mou P, Peng H, Zhou L, Li L, Li H, Huang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	calcium-deficient hydroxyapatite multi-(amino acid) copolymer copper osteogenesis degradability cytocompatibility Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/128e49d6f11d445bb3aa00dc3eee93e9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:128e49d6f11d445bb3aa00dc3eee93e9
record_format	dspace
spelling	oai:doaj.org-article:128e49d6f11d445bb3aa00dc3eee93e92021-12-02T00:58:52ZA novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration1178-2013https://doaj.org/article/128e49d6f11d445bb3aa00dc3eee93e92019-05-01T00:00:00Zhttps://www.dovepress.com/a-novel-composite-scaffold-of-cu-doped-nano-calcium-deficient-hydroxya-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ping Mou,1 Haitao Peng,2 Li Zhou,3 Lin Li,2 Hong Li,2 Qiang Huang11Department of Orthopedic Surgery, West China Hospital, West China Medical School, Sichuan University; 2School of Physical Science and Technology, Sichuan University; 3Core Facility of West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, People’s Republic of ChinaBackground and methods: A Cu-doped composite scaffold of nano calcium-deficient hydroxyapatite (n-CDHA)/multi(amino acid) copolymer (MAC) was prepared. The structure, porosity, morphology and compressive strength of the scaffolds were characterized, the in vitro degradability in phosphate-buffered solution (PBS) and cell responses to the scaffolds were investigated, and in vivo stimulation of bone formation were analyzed.Results: The scaffolds showed the compressive strength of approximately 12 MPa and total porosity of about 81%. Weight loss of the composite scaffolds was 63% after 16-week immersion in PBS. Cu release in scaffolds showed a marked dependence on the initial amount in the scaffolds over time. Cu-doped n-CDHA/MAC scaffolds with the content of Cu 0.5% and 1% in mass ratio showed better cell responses to proliferation and differentiation of rat bone marrow stromal cells (rBMSCs) than that with no Cu. After 12-week implantation in rabbits, 1% Cu-doped n-CDHA/MAC showed better ability of angiogenesis and osteogenesis compared to 0% Cu-doped n-CDHA/MAC.Conclusion: The 1% Cu-doped n-CDHA/MAC composite scaffold showed good capacity of angiogenesis and osteogenesis, and the Cu showed positive effects on cell growth and osteogenesis. And it has potential to be used as bone regeneration scaffolds.Keywords: calcium-deficient hydroxyapatite, multi-(amino acid) copolymer, copper, osteogenesis, degradability, cytocompatibilityMou PPeng HZhou LLi LLi HHuang QDove Medical Pressarticlecalcium-deficient hydroxyapatitemulti-(amino acid) copolymercopperosteogenesisdegradabilitycytocompatibilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3331-3343 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcium-deficient hydroxyapatite multi-(amino acid) copolymer copper osteogenesis degradability cytocompatibility Medicine (General) R5-920
spellingShingle	calcium-deficient hydroxyapatite multi-(amino acid) copolymer copper osteogenesis degradability cytocompatibility Medicine (General) R5-920 Mou P Peng H Zhou L Li L Li H Huang Q A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
description	Ping Mou,1 Haitao Peng,2 Li Zhou,3 Lin Li,2 Hong Li,2 Qiang Huang11Department of Orthopedic Surgery, West China Hospital, West China Medical School, Sichuan University; 2School of Physical Science and Technology, Sichuan University; 3Core Facility of West China Hospital, Sichuan University, Chengdu, Sichuan Province 610041, People’s Republic of ChinaBackground and methods: A Cu-doped composite scaffold of nano calcium-deficient hydroxyapatite (n-CDHA)/multi(amino acid) copolymer (MAC) was prepared. The structure, porosity, morphology and compressive strength of the scaffolds were characterized, the in vitro degradability in phosphate-buffered solution (PBS) and cell responses to the scaffolds were investigated, and in vivo stimulation of bone formation were analyzed.Results: The scaffolds showed the compressive strength of approximately 12 MPa and total porosity of about 81%. Weight loss of the composite scaffolds was 63% after 16-week immersion in PBS. Cu release in scaffolds showed a marked dependence on the initial amount in the scaffolds over time. Cu-doped n-CDHA/MAC scaffolds with the content of Cu 0.5% and 1% in mass ratio showed better cell responses to proliferation and differentiation of rat bone marrow stromal cells (rBMSCs) than that with no Cu. After 12-week implantation in rabbits, 1% Cu-doped n-CDHA/MAC showed better ability of angiogenesis and osteogenesis compared to 0% Cu-doped n-CDHA/MAC.Conclusion: The 1% Cu-doped n-CDHA/MAC composite scaffold showed good capacity of angiogenesis and osteogenesis, and the Cu showed positive effects on cell growth and osteogenesis. And it has potential to be used as bone regeneration scaffolds.Keywords: calcium-deficient hydroxyapatite, multi-(amino acid) copolymer, copper, osteogenesis, degradability, cytocompatibility
format	article
author	Mou P Peng H Zhou L Li L Li H Huang Q
author_facet	Mou P Peng H Zhou L Li L Li H Huang Q
author_sort	Mou P
title	A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
title_short	A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
title_full	A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
title_fullStr	A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
title_full_unstemmed	A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
title_sort	novel composite scaffold of cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/128e49d6f11d445bb3aa00dc3eee93e9
work_keys_str_mv	AT moup anovelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT pengh anovelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT zhoul anovelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT lil anovelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT lih anovelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT huangq anovelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT moup novelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT pengh novelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT zhoul novelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT lil novelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT lih novelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration AT huangq novelcompositescaffoldofcudopednanocalciumdeficienthydroxyapatitemultiaminoacidcopolymerforbonetissueregeneration
_version_	1718403362809970688

A novel composite scaffold of Cu-doped nano calcium-deficient hydroxyapatite/multi-(amino acid) copolymer for bone tissue regeneration

Ejemplares similares