The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae

The availability of next-generation sequencing (NGS) in recent years has facilitated a revolution in the availability of mitochondrial (mt) genome sequences. The mt genome is a powerful tool for comparative studies and resolving the phylogenetic relationships among insect lineages. The mt genomes of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sam Pedro Galilee Ayivi, Yao Tong, Kenneth B. Storey, Dan-Na Yu, Jia-Yong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Scarabaeidae phytophagous mitochondrial genome gene rearrangement non-coding region (NCR) phylogeny Science Q
Acceso en línea:	https://doaj.org/article/129096bc9a4d41d88e1360836d1f034d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:129096bc9a4d41d88e1360836d1f034d
record_format	dspace
spelling	oai:doaj.org-article:129096bc9a4d41d88e1360836d1f034d2021-11-25T17:59:53ZThe Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae10.3390/insects121110252075-4450https://doaj.org/article/129096bc9a4d41d88e1360836d1f034d2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/1025https://doaj.org/toc/2075-4450The availability of next-generation sequencing (NGS) in recent years has facilitated a revolution in the availability of mitochondrial (mt) genome sequences. The mt genome is a powerful tool for comparative studies and resolving the phylogenetic relationships among insect lineages. The mt genomes of phytophagous scarabs of the subfamilies Cetoniinae and Dynastinae were under-represented in GenBank. Previous research found that the subfamily Rutelinae was recovered as a paraphyletic group because the few representatives of the subfamily Dynastinae clustered into Rutelinae, but the subfamily position of Dynastinae was still unclear. In the present study, we sequenced 18 mt genomes from Dynastinae and Cetoniinae using next-generation sequencing (NGS) to re-assess the phylogenetic relationships within Scarabaeidae. All sequenced mt genomes contained 37 sets of genes (13 protein-coding genes, 22 tRNAs, and two ribosomal RNAs), with one long control region, but the gene order was not the same between Cetoniinae and Dynastinae species. All mt genomes of Dynastinae species showed the same gene rearrangement of trnQ-NCR-trnI-trnM, whereas all mt genomes of Cetoniinae species showed the ancestral insect gene order of trnI-trnQ-trnM. Phylogenetic analyses (IQ-tree and MrBayes) were conducted using 13 protein-coding genes based on nucleotide and amino acid datasets. In the ML and BI trees, we recovered the monophyly of Rutelinae, Cetoniinae, Dynastinae, and Sericinae, and the non-monophyly of Melolonthinae. Cetoniinae was shown to be a sister clade to (Dynastinae + Rutelinae).Sam Pedro Galilee AyiviYao TongKenneth B. StoreyDan-Na YuJia-Yong ZhangMDPI AGarticleScarabaeidaephytophagousmitochondrial genomegene rearrangementnon-coding region (NCR)phylogenyScienceQENInsects, Vol 12, Iss 1025, p 1025 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Scarabaeidae phytophagous mitochondrial genome gene rearrangement non-coding region (NCR) phylogeny Science Q
spellingShingle	Scarabaeidae phytophagous mitochondrial genome gene rearrangement non-coding region (NCR) phylogeny Science Q Sam Pedro Galilee Ayivi Yao Tong Kenneth B. Storey Dan-Na Yu Jia-Yong Zhang The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
description	The availability of next-generation sequencing (NGS) in recent years has facilitated a revolution in the availability of mitochondrial (mt) genome sequences. The mt genome is a powerful tool for comparative studies and resolving the phylogenetic relationships among insect lineages. The mt genomes of phytophagous scarabs of the subfamilies Cetoniinae and Dynastinae were under-represented in GenBank. Previous research found that the subfamily Rutelinae was recovered as a paraphyletic group because the few representatives of the subfamily Dynastinae clustered into Rutelinae, but the subfamily position of Dynastinae was still unclear. In the present study, we sequenced 18 mt genomes from Dynastinae and Cetoniinae using next-generation sequencing (NGS) to re-assess the phylogenetic relationships within Scarabaeidae. All sequenced mt genomes contained 37 sets of genes (13 protein-coding genes, 22 tRNAs, and two ribosomal RNAs), with one long control region, but the gene order was not the same between Cetoniinae and Dynastinae species. All mt genomes of Dynastinae species showed the same gene rearrangement of trnQ-NCR-trnI-trnM, whereas all mt genomes of Cetoniinae species showed the ancestral insect gene order of trnI-trnQ-trnM. Phylogenetic analyses (IQ-tree and MrBayes) were conducted using 13 protein-coding genes based on nucleotide and amino acid datasets. In the ML and BI trees, we recovered the monophyly of Rutelinae, Cetoniinae, Dynastinae, and Sericinae, and the non-monophyly of Melolonthinae. Cetoniinae was shown to be a sister clade to (Dynastinae + Rutelinae).
format	article
author	Sam Pedro Galilee Ayivi Yao Tong Kenneth B. Storey Dan-Na Yu Jia-Yong Zhang
author_facet	Sam Pedro Galilee Ayivi Yao Tong Kenneth B. Storey Dan-Na Yu Jia-Yong Zhang
author_sort	Sam Pedro Galilee Ayivi
title	The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
title_short	The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
title_full	The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
title_fullStr	The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
title_full_unstemmed	The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae
title_sort	mitochondrial genomes of 18 new pleurosticti (coleoptera: scarabaeidae) exhibit a novel trnq-ncr-trni-trnm gene rearrangement and clarify phylogenetic relationships of subfamilies within scarabaeidae
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/129096bc9a4d41d88e1360836d1f034d
work_keys_str_mv	AT sampedrogalileeayivi themitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT yaotong themitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT kennethbstorey themitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT dannayu themitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT jiayongzhang themitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT sampedrogalileeayivi mitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT yaotong mitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT kennethbstorey mitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT dannayu mitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae AT jiayongzhang mitochondrialgenomesof18newpleurosticticoleopterascarabaeidaeexhibitanoveltrnqncrtrnitrnmgenerearrangementandclarifyphylogeneticrelationshipsofsubfamilieswithinscarabaeidae
_version_	1718411724617416704

The Mitochondrial Genomes of 18 New Pleurosticti (Coleoptera: Scarabaeidae) Exhibit a Novel trnQ-NCR-trnI-trnM Gene Rearrangement and Clarify Phylogenetic Relationships of Subfamilies within Scarabaeidae

Ejemplares similares