Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain

Synapses are highly specialized structures that interconnect neurons to form functional networks dedicated to neuronal communication. During brain development, synapses undergo activity-dependent rearrangements leading to both structural and functional changes. Many molecular processes are involved...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie Pronot, Félicie Kieffer, Anne-Sophie Gay, Delphine Debayle, Raphaël Forquet, Gwénola Poupon, Lenka Schorova, Stéphane Martin, Carole Gwizdek
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	synapse post-translational modification SUMO SUMOylome proteomics Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/12953524713c4626b238a6cd7af1dd80
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:12953524713c4626b238a6cd7af1dd80
record_format	dspace
spelling	oai:doaj.org-article:12953524713c4626b238a6cd7af1dd802021-11-30T14:41:24ZProteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain1662-509910.3389/fnmol.2021.780535https://doaj.org/article/12953524713c4626b238a6cd7af1dd802021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnmol.2021.780535/fullhttps://doaj.org/toc/1662-5099Synapses are highly specialized structures that interconnect neurons to form functional networks dedicated to neuronal communication. During brain development, synapses undergo activity-dependent rearrangements leading to both structural and functional changes. Many molecular processes are involved in this regulation, including post-translational modifications by the Small Ubiquitin-like MOdifier SUMO. To get a wider view of the panel of endogenous synaptic SUMO-modified proteins in the mammalian brain, we combined subcellular fractionation of rat brains at the post-natal day 14 with denaturing immunoprecipitation using SUMO2/3 antibodies and tandem mass spectrometry analysis. Our screening identified 803 candidate SUMO2/3 targets, which represents about 18% of the synaptic proteome. Our dataset includes neurotransmitter receptors, transporters, adhesion molecules, scaffolding proteins as well as vesicular trafficking and cytoskeleton-associated proteins, defining SUMO2/3 as a central regulator of the synaptic organization and function.Marie PronotFélicie KiefferAnne-Sophie GayDelphine DebayleRaphaël ForquetGwénola PouponLenka SchorovaStéphane MartinCarole GwizdekFrontiers Media S.A.articlesynapsepost-translational modificationSUMOSUMOylomeproteomicsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Molecular Neuroscience, Vol 14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synapse post-translational modification SUMO SUMOylome proteomics Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	synapse post-translational modification SUMO SUMOylome proteomics Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Marie Pronot Félicie Kieffer Anne-Sophie Gay Delphine Debayle Raphaël Forquet Gwénola Poupon Lenka Schorova Stéphane Martin Carole Gwizdek Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain
description	Synapses are highly specialized structures that interconnect neurons to form functional networks dedicated to neuronal communication. During brain development, synapses undergo activity-dependent rearrangements leading to both structural and functional changes. Many molecular processes are involved in this regulation, including post-translational modifications by the Small Ubiquitin-like MOdifier SUMO. To get a wider view of the panel of endogenous synaptic SUMO-modified proteins in the mammalian brain, we combined subcellular fractionation of rat brains at the post-natal day 14 with denaturing immunoprecipitation using SUMO2/3 antibodies and tandem mass spectrometry analysis. Our screening identified 803 candidate SUMO2/3 targets, which represents about 18% of the synaptic proteome. Our dataset includes neurotransmitter receptors, transporters, adhesion molecules, scaffolding proteins as well as vesicular trafficking and cytoskeleton-associated proteins, defining SUMO2/3 as a central regulator of the synaptic organization and function.
format	article
author	Marie Pronot Félicie Kieffer Anne-Sophie Gay Delphine Debayle Raphaël Forquet Gwénola Poupon Lenka Schorova Stéphane Martin Carole Gwizdek
author_facet	Marie Pronot Félicie Kieffer Anne-Sophie Gay Delphine Debayle Raphaël Forquet Gwénola Poupon Lenka Schorova Stéphane Martin Carole Gwizdek
author_sort	Marie Pronot
title	Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain
title_short	Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain
title_full	Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain
title_fullStr	Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain
title_full_unstemmed	Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain
title_sort	proteomic identification of an endogenous synaptic sumoylome in the developing rat brain
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/12953524713c4626b238a6cd7af1dd80
work_keys_str_mv	AT mariepronot proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT feliciekieffer proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT annesophiegay proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT delphinedebayle proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT raphaelforquet proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT gwenolapoupon proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT lenkaschorova proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT stephanemartin proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain AT carolegwizdek proteomicidentificationofanendogenoussynapticsumoylomeinthedevelopingratbrain
_version_	1718406487550722048

Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain

Ejemplares similares