Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.

Precise patterning of morphogen molecules and their accurate reading out are of key importance in embryonic development. Recent experiments have visualized distributions of proteins in developing embryos and shown that the gradient of concentration of Bicoid morphogen in Drosophila embryos is establ...

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Autores principales:	Yurie Okabe-Oho, Hiroki Murakami, Suguru Oho, Masaki Sasai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1299d74d6f664563bd6630629641cb87
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spelling	oai:doaj.org-article:1299d74d6f664563bd6630629641cb872021-11-25T05:42:12ZStable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.1553-734X1553-735810.1371/journal.pcbi.1000486https://doaj.org/article/1299d74d6f664563bd6630629641cb872009-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19714200/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Precise patterning of morphogen molecules and their accurate reading out are of key importance in embryonic development. Recent experiments have visualized distributions of proteins in developing embryos and shown that the gradient of concentration of Bicoid morphogen in Drosophila embryos is established rapidly after fertilization and remains stable through syncytial mitoses. This stable Bicoid gradient is read out in a precise way to distribute Hunchback with small fluctuations in each embryo and in a reproducible way, with small embryo-to-embryo fluctuation. The mechanisms of such stable, precise, and reproducible patterning through noisy cellular processes, however, still remain mysterious. To address these issues, here we develop the one- and three-dimensional stochastic models of the early Drosophila embryo. The simulated results show that the fluctuation in expression of the hunchback gene is dominated by the random arrival of Bicoid at the hunchback enhancer. Slow diffusion of Hunchback protein, however, averages out this intense fluctuation, leading to the precise patterning of distribution of Hunchback without loss of sharpness of the boundary of its distribution. The coordinated rates of diffusion and transport of input Bicoid and output Hunchback play decisive roles in suppressing fluctuations arising from the dynamical structure change in embryos and those arising from the random diffusion of molecules, and give rise to the stable, precise, and reproducible patterning of Bicoid and Hunchback distributions.Yurie Okabe-OhoHiroki MurakamiSuguru OhoMasaki SasaiPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 5, Iss 8, p e1000486 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yurie Okabe-Oho Hiroki Murakami Suguru Oho Masaki Sasai Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.
description	Precise patterning of morphogen molecules and their accurate reading out are of key importance in embryonic development. Recent experiments have visualized distributions of proteins in developing embryos and shown that the gradient of concentration of Bicoid morphogen in Drosophila embryos is established rapidly after fertilization and remains stable through syncytial mitoses. This stable Bicoid gradient is read out in a precise way to distribute Hunchback with small fluctuations in each embryo and in a reproducible way, with small embryo-to-embryo fluctuation. The mechanisms of such stable, precise, and reproducible patterning through noisy cellular processes, however, still remain mysterious. To address these issues, here we develop the one- and three-dimensional stochastic models of the early Drosophila embryo. The simulated results show that the fluctuation in expression of the hunchback gene is dominated by the random arrival of Bicoid at the hunchback enhancer. Slow diffusion of Hunchback protein, however, averages out this intense fluctuation, leading to the precise patterning of distribution of Hunchback without loss of sharpness of the boundary of its distribution. The coordinated rates of diffusion and transport of input Bicoid and output Hunchback play decisive roles in suppressing fluctuations arising from the dynamical structure change in embryos and those arising from the random diffusion of molecules, and give rise to the stable, precise, and reproducible patterning of Bicoid and Hunchback distributions.
format	article
author	Yurie Okabe-Oho Hiroki Murakami Suguru Oho Masaki Sasai
author_facet	Yurie Okabe-Oho Hiroki Murakami Suguru Oho Masaki Sasai
author_sort	Yurie Okabe-Oho
title	Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.
title_short	Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.
title_full	Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.
title_fullStr	Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.
title_full_unstemmed	Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.
title_sort	stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early drosophila embryo.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/1299d74d6f664563bd6630629641cb87
work_keys_str_mv	AT yurieokabeoho stablepreciseandreproduciblepatterningofbicoidandhunchbackmoleculesintheearlydrosophilaembryo AT hirokimurakami stablepreciseandreproduciblepatterningofbicoidandhunchbackmoleculesintheearlydrosophilaembryo AT suguruoho stablepreciseandreproduciblepatterningofbicoidandhunchbackmoleculesintheearlydrosophilaembryo AT masakisasai stablepreciseandreproduciblepatterningofbicoidandhunchbackmoleculesintheearlydrosophilaembryo
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Stable, precise, and reproducible patterning of bicoid and hunchback molecules in the early Drosophila embryo.

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