Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health

Abstract Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were signifi...

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Autores principales:	Martina Tambassi, Melissa Berni, Chiara Bracchi, Erika Scaltriti, Marina Morganti, Luca Bolzoni, Jennifer R. Tanner, Gaëtan Thilliez, Robert A. Kingsley, Stefano Pongolini, Gabriele Casadei
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/129ad161b59b4c36a5a84aad6931a6d0
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spelling	oai:doaj.org-article:129ad161b59b4c36a5a84aad6931a6d02021-12-02T12:33:05ZMutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health10.1038/s41598-020-78443-72045-2322https://doaj.org/article/129ad161b59b4c36a5a84aad6931a6d02020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78443-7https://doaj.org/toc/2045-2322Abstract Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were significantly under-represented in human infections, consistent with further host adaptation to swine. Here we show that isolates with this PFGE profile form a distinct phylogenetic sub-clade within S. Derby and exhibit a profound reduction in invasion of human epithelial cells, and a relatively small reduction in swine epithelial cells. A single missense mutation in hilD, that encodes the master-regulator of the Salmonella Pathogenicity Island 1 (SPI-1), was present in the adapted lineage. The missense mutation resulted in a loss of function of HilD that accounted for reduced invasion in human epithelial cells. The relatively small impact of the mutation on interaction with swine cells was consistent with an alternative mechanism of invasion in this pathogen-host combination.Martina TambassiMelissa BerniChiara BracchiErika ScaltritiMarina MorgantiLuca BolzoniJennifer R. TannerGaëtan ThilliezRobert A. KingsleyStefano PongoliniGabriele CasadeiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martina Tambassi Melissa Berni Chiara Bracchi Erika Scaltriti Marina Morganti Luca Bolzoni Jennifer R. Tanner Gaëtan Thilliez Robert A. Kingsley Stefano Pongolini Gabriele Casadei Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
description	Abstract Salmonella enterica variants exhibit diverse host adaptation, outcome of infection, and associated risk to food safety. Analysis of the distribution of Salmonella enterica serovar Derby (S. Derby) subtypes in human and swine identified isolates with a distinct PFGE profile that were significantly under-represented in human infections, consistent with further host adaptation to swine. Here we show that isolates with this PFGE profile form a distinct phylogenetic sub-clade within S. Derby and exhibit a profound reduction in invasion of human epithelial cells, and a relatively small reduction in swine epithelial cells. A single missense mutation in hilD, that encodes the master-regulator of the Salmonella Pathogenicity Island 1 (SPI-1), was present in the adapted lineage. The missense mutation resulted in a loss of function of HilD that accounted for reduced invasion in human epithelial cells. The relatively small impact of the mutation on interaction with swine cells was consistent with an alternative mechanism of invasion in this pathogen-host combination.
format	article
author	Martina Tambassi Melissa Berni Chiara Bracchi Erika Scaltriti Marina Morganti Luca Bolzoni Jennifer R. Tanner Gaëtan Thilliez Robert A. Kingsley Stefano Pongolini Gabriele Casadei
author_facet	Martina Tambassi Melissa Berni Chiara Bracchi Erika Scaltriti Marina Morganti Luca Bolzoni Jennifer R. Tanner Gaëtan Thilliez Robert A. Kingsley Stefano Pongolini Gabriele Casadei
author_sort	Martina Tambassi
title	Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
title_short	Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
title_full	Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
title_fullStr	Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
title_full_unstemmed	Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health
title_sort	mutation of hild in a salmonella derby lineage linked to swine adaptation and reduced risk to human health
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/129ad161b59b4c36a5a84aad6931a6d0
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Mutation of hilD in a Salmonella Derby lineage linked to swine adaptation and reduced risk to human health

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