Multi-frequency passive and active microrheology with optical tweezers

Abstract Optical tweezers have attracted significant attention for microrheological applications, due to the possibility of investigating viscoelastic properties in vivo which are strongly related to the health status and development of biological specimens. In order to use optical tweezers as a mic...

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Autores principales:	Randhir Kumar, Valerio Vitali, Timo Wiedemann, Robert Meissner, Paolo Minzioni, Cornelia Denz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/129b28a33ff2445fb75fc4987c114cb4
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spelling	oai:doaj.org-article:129b28a33ff2445fb75fc4987c114cb42021-12-02T15:23:17ZMulti-frequency passive and active microrheology with optical tweezers10.1038/s41598-021-93130-x2045-2322https://doaj.org/article/129b28a33ff2445fb75fc4987c114cb42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93130-xhttps://doaj.org/toc/2045-2322Abstract Optical tweezers have attracted significant attention for microrheological applications, due to the possibility of investigating viscoelastic properties in vivo which are strongly related to the health status and development of biological specimens. In order to use optical tweezers as a microrheological tool, an exact force calibration in the complex system under investigation is required. One of the most promising techniques for optical tweezers calibration in a viscoelastic medium is the so-called active–passive calibration, which allows determining both the trap stiffness and microrheological properties of the medium with the least a-priori knowledge in comparison to the other methods. In this manuscript, we develop an optimization of the active–passive calibration technique performed with a sample stage driving, whose implementation is more straightforward with respect to standard laser driving where two different laser beams are required. We performed microrheological measurements over a broad frequency range in a few seconds implementing an accurate multi-frequency driving of the sample stage. The optical tweezers-based microrheometer was first validated by measuring water, and then exemplarily applied to more viscous medium and subsequently to a viscoelastic solution of methylcellulose in water. The described method paves the way to microrheological precision metrology in biological samples with high temporal- and spatial-resolution allowing for investigation of even short time-scale phenomena.Randhir KumarValerio VitaliTimo WiedemannRobert MeissnerPaolo MinzioniCornelia DenzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Randhir Kumar Valerio Vitali Timo Wiedemann Robert Meissner Paolo Minzioni Cornelia Denz Multi-frequency passive and active microrheology with optical tweezers
description	Abstract Optical tweezers have attracted significant attention for microrheological applications, due to the possibility of investigating viscoelastic properties in vivo which are strongly related to the health status and development of biological specimens. In order to use optical tweezers as a microrheological tool, an exact force calibration in the complex system under investigation is required. One of the most promising techniques for optical tweezers calibration in a viscoelastic medium is the so-called active–passive calibration, which allows determining both the trap stiffness and microrheological properties of the medium with the least a-priori knowledge in comparison to the other methods. In this manuscript, we develop an optimization of the active–passive calibration technique performed with a sample stage driving, whose implementation is more straightforward with respect to standard laser driving where two different laser beams are required. We performed microrheological measurements over a broad frequency range in a few seconds implementing an accurate multi-frequency driving of the sample stage. The optical tweezers-based microrheometer was first validated by measuring water, and then exemplarily applied to more viscous medium and subsequently to a viscoelastic solution of methylcellulose in water. The described method paves the way to microrheological precision metrology in biological samples with high temporal- and spatial-resolution allowing for investigation of even short time-scale phenomena.
format	article
author	Randhir Kumar Valerio Vitali Timo Wiedemann Robert Meissner Paolo Minzioni Cornelia Denz
author_facet	Randhir Kumar Valerio Vitali Timo Wiedemann Robert Meissner Paolo Minzioni Cornelia Denz
author_sort	Randhir Kumar
title	Multi-frequency passive and active microrheology with optical tweezers
title_short	Multi-frequency passive and active microrheology with optical tweezers
title_full	Multi-frequency passive and active microrheology with optical tweezers
title_fullStr	Multi-frequency passive and active microrheology with optical tweezers
title_full_unstemmed	Multi-frequency passive and active microrheology with optical tweezers
title_sort	multi-frequency passive and active microrheology with optical tweezers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/129b28a33ff2445fb75fc4987c114cb4
work_keys_str_mv	AT randhirkumar multifrequencypassiveandactivemicrorheologywithopticaltweezers AT valeriovitali multifrequencypassiveandactivemicrorheologywithopticaltweezers AT timowiedemann multifrequencypassiveandactivemicrorheologywithopticaltweezers AT robertmeissner multifrequencypassiveandactivemicrorheologywithopticaltweezers AT paolominzioni multifrequencypassiveandactivemicrorheologywithopticaltweezers AT corneliadenz multifrequencypassiveandactivemicrorheologywithopticaltweezers
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Multi-frequency passive and active microrheology with optical tweezers

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