The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

Saad M Ahsan, Chintalagiri Mohan Rao Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, Telangana, India Abstract: The process of moving hydrophobic amino acids into the core of a protein by desolvation is important in protein folding. However, a r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ahsan SM, Rao CM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Protein desolvation nanoparticle assembly gelatin nanoparticle synthesis protease susceptibility intra-cellular drug release Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/129c56b745154401a53e0a6b1c4e3370
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:129c56b745154401a53e0a6b1c4e3370
record_format	dspace
spelling	oai:doaj.org-article:129c56b745154401a53e0a6b1c4e33702021-12-02T06:33:30ZThe role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release1178-2013https://doaj.org/article/129c56b745154401a53e0a6b1c4e33702017-01-01T00:00:00Zhttps://www.dovepress.com/the-role-of-surface-charge-in-the-desolvation-process-of-gelatin-impli-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Saad M Ahsan, Chintalagiri Mohan Rao Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, Telangana, India Abstract: The process of moving hydrophobic amino acids into the core of a protein by desolvation is important in protein folding. However, a rapid and forced desolvation can lead to precipitation of proteins. Desolvation of proteins under controlled conditions generates nanoparticles – homogeneous aggregates with a narrow size distribution. The protein nanoparticles, under physiological conditions, undergo surface erosion due to the action of proteases, releasing the entrapped drug/gene. The packing density of protein nanoparticles significantly influences the release kinetics. We have investigated the desolvation process of gelatin, exploring the role of pH and desolvating agent in nanoparticle synthesis. Our results show that the desolvation process, initiated by the addition of acetone, follows distinct pathways for gelatin incubated at different pH values and results in the generation of nanoparticles with varying matrix densities. The nanoparticles synthesized with varying matrix densities show variations in drug loading and protease-dependent extra- and intracellular drug release. These results will be useful in fine-tuning the synthesis of nanoparticles with desirable drug release profiles. Keywords: protein desolvation, nanoparticle assembly, gelatin nanoparticle synthesis, protease susceptibility, intracellular drug releaseAhsan SMRao CMDove Medical PressarticleProtein desolvationnanoparticle assemblygelatin nanoparticle synthesisprotease susceptibilityintra-cellular drug releaseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 795-808 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Protein desolvation nanoparticle assembly gelatin nanoparticle synthesis protease susceptibility intra-cellular drug release Medicine (General) R5-920
spellingShingle	Protein desolvation nanoparticle assembly gelatin nanoparticle synthesis protease susceptibility intra-cellular drug release Medicine (General) R5-920 Ahsan SM Rao CM The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
description	Saad M Ahsan, Chintalagiri Mohan Rao Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, Telangana, India Abstract: The process of moving hydrophobic amino acids into the core of a protein by desolvation is important in protein folding. However, a rapid and forced desolvation can lead to precipitation of proteins. Desolvation of proteins under controlled conditions generates nanoparticles – homogeneous aggregates with a narrow size distribution. The protein nanoparticles, under physiological conditions, undergo surface erosion due to the action of proteases, releasing the entrapped drug/gene. The packing density of protein nanoparticles significantly influences the release kinetics. We have investigated the desolvation process of gelatin, exploring the role of pH and desolvating agent in nanoparticle synthesis. Our results show that the desolvation process, initiated by the addition of acetone, follows distinct pathways for gelatin incubated at different pH values and results in the generation of nanoparticles with varying matrix densities. The nanoparticles synthesized with varying matrix densities show variations in drug loading and protease-dependent extra- and intracellular drug release. These results will be useful in fine-tuning the synthesis of nanoparticles with desirable drug release profiles. Keywords: protein desolvation, nanoparticle assembly, gelatin nanoparticle synthesis, protease susceptibility, intracellular drug release
format	article
author	Ahsan SM Rao CM
author_facet	Ahsan SM Rao CM
author_sort	Ahsan SM
title	The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
title_short	The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
title_full	The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
title_fullStr	The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
title_full_unstemmed	The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
title_sort	role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/129c56b745154401a53e0a6b1c4e3370
work_keys_str_mv	AT ahsansm theroleofsurfacechargeinthedesolvationprocessofgelatinimplicationsinnanoparticlesynthesisandmodulationofdrugrelease AT raocm theroleofsurfacechargeinthedesolvationprocessofgelatinimplicationsinnanoparticlesynthesisandmodulationofdrugrelease AT ahsansm roleofsurfacechargeinthedesolvationprocessofgelatinimplicationsinnanoparticlesynthesisandmodulationofdrugrelease AT raocm roleofsurfacechargeinthedesolvationprocessofgelatinimplicationsinnanoparticlesynthesisandmodulationofdrugrelease
_version_	1718399859422134272

The role of surface charge in the desolvation process of gelatin: implications in nanoparticle synthesis and modulation of drug release

Ejemplares similares