Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus

The parasitoid fungus Cordyceps cicadae, whose fruiting bodies are known in China as “chan hua,” literally “cicada flower,” has been used as a traditional Chinese medicinal ingredient for centuries. However, systematic disclosure of the vital factors responsible for the formation of wild cicada flow...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ailin Huang, Tao Wu, Xiuyun Wu, Biao Zhang, Yuanyuan Shen, Suying Wang, Wenjun Song, Haihua Ruan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	adenosine artificial cultivation microbial community N6-(2-hydroxyethyl)-adenosine soil physicochemical properties Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/129d3d5c2cc545318fe2b570b2d36ae9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:129d3d5c2cc545318fe2b570b2d36ae9
record_format	dspace
spelling	oai:doaj.org-article:129d3d5c2cc545318fe2b570b2d36ae92021-12-01T01:27:06ZAnalysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus1664-302X10.3389/fmicb.2021.752791https://doaj.org/article/129d3d5c2cc545318fe2b570b2d36ae92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.752791/fullhttps://doaj.org/toc/1664-302XThe parasitoid fungus Cordyceps cicadae, whose fruiting bodies are known in China as “chan hua,” literally “cicada flower,” has been used as a traditional Chinese medicinal ingredient for centuries. However, systematic disclosure of the vital factors responsible for the formation of wild cicada flower is limited. Here, we determined the physicochemical properties of soil and simultaneously analyzed the diversities and the structures of microbial community inhabiting the coremia, sclerotia, and soil around wild cicada flowers through high-throughput sequencing. Our results indicated that cicada flower more preferentially occurred in acidic soil (pH 5.9) with abundant moisture content (MC), total nitrogen (TN), and organic matter (OM). The dominant fungal genera in soil mainly included Isaria, f__Clavariaceae_Unclassified, Umbelopsis, f__Chaetomiaceae_Unclassified, Mortierella, f__Sordariaceae_Unclassified, and Arcopilus. Among them, C. cicadae was the only fungus that was massively detected in both the coremia and sclerotia with abundance of 83.5 and 53.6%, respectively. Based on this, a C. cicadae strain named AH10-4 with excellent adenosine- and N6-(2-hydroxyethyl)-adenosine (HEA)-producing capability was successfully isolated. However, to the aspect of bacteria, Burkholderia–Caballeronia–Paraburkholderia, Bacillus, Acidibacter, f__Xanthobacteraceae_Unclassified, and Candidatus_Solibacter were the dominant genera in soil. Pedobacter, f__Enterobacteriaceae_Unclassified, Pandoraea, Achromobacter, Stenotrophomonas, Burkholderia–Caballeronia–Paraburkholderia, and Chitinophaga were the dominant genera in the coremia and sclerotia. Notably, Burkholderia–Caballeronia–Paraburkholderia was the shared bacteria among them with high abundance of 3.1, 11.4, and 5.2% in the sclerotia, coremia, and soil, respectively. However, the possible role of these bacteria to the occurrence of cicada flower has been unclear to our knowledge. By analyzing the correlation between physicochemical properties and microbial community of soil, we found that MC, Fe, and Zn were significantly negatively correlated with soil Isaria and that Cu was significantly negatively correlated with most dominant soil bacterial genera. But Mg was significantly positively correlated with most dominant taxa. This study provides new insight into the formation mechanisms of cicada flower and may contribute to the large-scale cultivation of cicada flowers.Ailin HuangTao WuXiuyun WuBiao ZhangYuanyuan ShenSuying WangWenjun SongHaihua RuanFrontiers Media S.A.articleadenosineartificial cultivationmicrobial communityN6-(2-hydroxyethyl)-adenosinesoil physicochemical propertiesMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adenosine artificial cultivation microbial community N6-(2-hydroxyethyl)-adenosine soil physicochemical properties Microbiology QR1-502
spellingShingle	adenosine artificial cultivation microbial community N6-(2-hydroxyethyl)-adenosine soil physicochemical properties Microbiology QR1-502 Ailin Huang Tao Wu Xiuyun Wu Biao Zhang Yuanyuan Shen Suying Wang Wenjun Song Haihua Ruan Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus
description	The parasitoid fungus Cordyceps cicadae, whose fruiting bodies are known in China as “chan hua,” literally “cicada flower,” has been used as a traditional Chinese medicinal ingredient for centuries. However, systematic disclosure of the vital factors responsible for the formation of wild cicada flower is limited. Here, we determined the physicochemical properties of soil and simultaneously analyzed the diversities and the structures of microbial community inhabiting the coremia, sclerotia, and soil around wild cicada flowers through high-throughput sequencing. Our results indicated that cicada flower more preferentially occurred in acidic soil (pH 5.9) with abundant moisture content (MC), total nitrogen (TN), and organic matter (OM). The dominant fungal genera in soil mainly included Isaria, f__Clavariaceae_Unclassified, Umbelopsis, f__Chaetomiaceae_Unclassified, Mortierella, f__Sordariaceae_Unclassified, and Arcopilus. Among them, C. cicadae was the only fungus that was massively detected in both the coremia and sclerotia with abundance of 83.5 and 53.6%, respectively. Based on this, a C. cicadae strain named AH10-4 with excellent adenosine- and N6-(2-hydroxyethyl)-adenosine (HEA)-producing capability was successfully isolated. However, to the aspect of bacteria, Burkholderia–Caballeronia–Paraburkholderia, Bacillus, Acidibacter, f__Xanthobacteraceae_Unclassified, and Candidatus_Solibacter were the dominant genera in soil. Pedobacter, f__Enterobacteriaceae_Unclassified, Pandoraea, Achromobacter, Stenotrophomonas, Burkholderia–Caballeronia–Paraburkholderia, and Chitinophaga were the dominant genera in the coremia and sclerotia. Notably, Burkholderia–Caballeronia–Paraburkholderia was the shared bacteria among them with high abundance of 3.1, 11.4, and 5.2% in the sclerotia, coremia, and soil, respectively. However, the possible role of these bacteria to the occurrence of cicada flower has been unclear to our knowledge. By analyzing the correlation between physicochemical properties and microbial community of soil, we found that MC, Fe, and Zn were significantly negatively correlated with soil Isaria and that Cu was significantly negatively correlated with most dominant soil bacterial genera. But Mg was significantly positively correlated with most dominant taxa. This study provides new insight into the formation mechanisms of cicada flower and may contribute to the large-scale cultivation of cicada flowers.
format	article
author	Ailin Huang Tao Wu Xiuyun Wu Biao Zhang Yuanyuan Shen Suying Wang Wenjun Song Haihua Ruan
author_facet	Ailin Huang Tao Wu Xiuyun Wu Biao Zhang Yuanyuan Shen Suying Wang Wenjun Song Haihua Ruan
author_sort	Ailin Huang
title	Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus
title_short	Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus
title_full	Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus
title_fullStr	Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus
title_full_unstemmed	Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus
title_sort	analysis of internal and external microorganism community of wild cicada flowers and identification of the predominant cordyceps cicadae fungus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/129d3d5c2cc545318fe2b570b2d36ae9
work_keys_str_mv	AT ailinhuang analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT taowu analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT xiuyunwu analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT biaozhang analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT yuanyuanshen analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT suyingwang analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT wenjunsong analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus AT haihuaruan analysisofinternalandexternalmicroorganismcommunityofwildcicadaflowersandidentificationofthepredominantcordycepscicadaefungus
_version_	1718405944842387456

Analysis of Internal and External Microorganism Community of Wild Cicada Flowers and Identification of the Predominant Cordyceps cicadae Fungus

Ejemplares similares