Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells

Sankarathi Balaiya, Ravi K Murthy, Vikram&nbsp;S Brar, Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.Methods: RGC-5 and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sankarathi Balaiya, Ravi K Murthy, Vikram&nbsp;S Brar, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/12a0f6c3c67e4bc29958438114abf9e4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:12a0f6c3c67e4bc29958438114abf9e4
record_format	dspace
spelling	oai:doaj.org-article:12a0f6c3c67e4bc29958438114abf9e42021-12-02T02:27:53ZEvaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells1177-54671177-5483https://doaj.org/article/12a0f6c3c67e4bc29958438114abf9e42010-01-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-ultraviolet-light-toxicity-on-cultured-retinal-pigment-e-a3899https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Sankarathi Balaiya, Ravi K Murthy, Vikram&nbsp;S Brar, Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.Methods: RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.Results: Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS. Increased duration of exposure (&gt;15 minutes), was associated with increased expression of bax and cytochrome C, and absence of bcl-2 expression.Conclusion: UVB light exposure results in cell cytotoxicity. The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.Keywords: ultraviolet light, retinal pigment epithelium, retinal ganglion cell, reactive oxygen species, cytochrome C Sankarathi BalaiyaRavi K MurthyVikram&nbsp;S Braret alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 33-39 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Sankarathi Balaiya Ravi K Murthy Vikram&nbsp;S Brar et al Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
description	Sankarathi Balaiya, Ravi K Murthy, Vikram&nbsp;S Brar, Kakarla V ChalamDepartment of Ophthalmology, University of Florida College of Medicine, Jacksonville, FL, USAPurpose: Our study is aimed at evaluating the role of UVB light in inducing cytotoxicity in an in vitro model.Methods: RGC-5 and ARPE-19 cells were exposed to different time periods of UVB light: 0, 15, 30, and 45 min. They were subsequently examined for changes in cell morphology, cell viability (neutral red uptake assay), generation of reactive oxygen species (ROS), expression of bax, bcl-2 and cytochome C by reverse transcriptase polymerase chain reaction and western blot, respectively.Results: Dose-dependent reduction in cell viability to UVB light was demonstrated with parallel increase in ROS. Increased duration of exposure (&gt;15 minutes), was associated with increased expression of bax and cytochrome C, and absence of bcl-2 expression.Conclusion: UVB light exposure results in cell cytotoxicity. The concomitant generation of ROS and expression of apoptotic markers suggests the role of oxidative stress in UVB-mediated apoptosis in an in vitro model of retinal ganglion and pigment epithelial cells.Keywords: ultraviolet light, retinal pigment epithelium, retinal ganglion cell, reactive oxygen species, cytochrome C
format	article
author	Sankarathi Balaiya Ravi K Murthy Vikram&nbsp;S Brar et al
author_facet	Sankarathi Balaiya Ravi K Murthy Vikram&nbsp;S Brar et al
author_sort	Sankarathi Balaiya
title	Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
title_short	Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
title_full	Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
title_fullStr	Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
title_full_unstemmed	Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
title_sort	evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/12a0f6c3c67e4bc29958438114abf9e4
work_keys_str_mv	AT sankarathibalaiya evaluationofultravioletlighttoxicityonculturedretinalpigmentepithelialandretinalganglioncells AT ravikmurthy evaluationofultravioletlighttoxicityonculturedretinalpigmentepithelialandretinalganglioncells AT vikramampnbspsbrar evaluationofultravioletlighttoxicityonculturedretinalpigmentepithelialandretinalganglioncells AT etal evaluationofultravioletlighttoxicityonculturedretinalpigmentepithelialandretinalganglioncells
_version_	1718402451340525568

Evaluation of ultraviolet light toxicity on cultured retinal pigment epithelial and retinal ganglion cells

Ejemplares similares