Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration
Background. Ultrasound is actively used to influence living organisms, but its physiological effect remains not fully understoodIn connection with the ability of ultrasonic waves to generate reactive oxygen species in an aqueous medium, special attention, from our point of view, should be paid to...
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oai:doaj.org-article:12a4379e55644e0c84cf9a4a9bf133852021-11-24T10:04:57ZOxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration10.21685/2307-9150-2021-3-22307-9150https://doaj.org/article/12a4379e55644e0c84cf9a4a9bf133852021-11-01T00:00:00Zhttps://doaj.org/toc/2307-9150Background. Ultrasound is actively used to influence living organisms, but its physiological effect remains not fully understoodIn connection with the ability of ultrasonic waves to generate reactive oxygen species in an aqueous medium, special attention, from our point of view, should be paid to oxidative homeostasis and primary proteolysis of a reserve nutrient in germinating seeds. As the main indicators, attention should be paid to the proteins’ oxidative modification (POM), since they are the main traps of bioradicals, but their turnover in plant tissues remains unexplored. The purpose of the research is to study the effect of different times of ultrasonic exposure on the level of POM, lipid peroxidation (LPO), cysteine proteinase activity and expression of its gene (CP) in germinating wheat seeds. Materials and methods. Wheat seeds (Triticum aestivum L.), “Ekada-70” variety, collected in 2018, were used as the object of the study. The seeds were placed in an aqueous medium of an ultrasonic bath “UNITRA – UNIMA” UM – 4. The treatment was carried out for 5, 10, and 20 minutes; seeds soaked but not sonicated served as a control. At the end, the level of lipid peroxidation was determined in the seeds by the level of malonic dialdehyde (MDA) concentration, OMB registration of 2,4 – denitrophenylhydrazones (2,4 – DNPH), cysteine proteinase activity and gene expression (CP). Results. The experiments revealed the dependence of the studied parameters on the time of ultrasonic exposure. An increase in MDA content in germinating wheat seeds was shown after ultrasonic exposure. The content of 2,4 – DNPH had a wave-like dynamics, did not statistically significantly change in germinating seeds exposed to ultrasound for 5 min, increased in samples that were exposed to ultrasound for 10 min, and decreased in seeds treated with ultrasound for 20 minutes. The activity of the studied proteinase was higher in the samples after 5 minutes of ultrasonic treatment and lower than the control values in the seeds after 20 minutes of sonication. Gene expression (SR) was higher in germinating seeds subjected to 5–10 minutes ultrasonic treatment, followed by a fall below the control. Conclusions. The intensification of LPO processes, the wave-like dynamics of POM and the primary activation of proteolysis with subsequent inhibition in germinating wheat seeds after ultrasound exposure were established; protein homeostasis is more tolerant to the action of ROS compared to lipids.S.S. TarasovA.P. VeselovPenza State University Publishing Housearticleoxidative modification of proteinslipid peroxidationcysteine proteinasesseeds of wheatseed germinationultrasoundBiology (General)QH301-705.5ENRUИзвестия высших учебных заведений. Поволжский регион: Естественные науки, Iss 3 (2021) |
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EN RU |
| topic |
oxidative modification of proteins lipid peroxidation cysteine proteinases seeds of wheat seed germination ultrasound Biology (General) QH301-705.5 |
| spellingShingle |
oxidative modification of proteins lipid peroxidation cysteine proteinases seeds of wheat seed germination ultrasound Biology (General) QH301-705.5 S.S. Tarasov A.P. Veselov Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| description |
Background. Ultrasound is actively used to influence living organisms, but its
physiological effect remains not fully understoodIn connection with the ability of ultrasonic
waves to generate reactive oxygen species in an aqueous medium, special attention, from
our point of view, should be paid to oxidative homeostasis and primary proteolysis of
a reserve nutrient in germinating seeds. As the main indicators, attention should be paid to
the proteins’ oxidative modification (POM), since they are the main traps of bioradicals, but
their turnover in plant tissues remains unexplored. The purpose of the research is to study
the effect of different times of ultrasonic exposure on the level of POM, lipid peroxidation
(LPO), cysteine proteinase activity and expression of its gene (CP) in germinating
wheat seeds. Materials and methods. Wheat seeds (Triticum aestivum L.), “Ekada-70” variety,
collected in 2018, were used as the object of the study. The seeds were placed in an
aqueous medium of an ultrasonic bath “UNITRA – UNIMA” UM – 4. The treatment was
carried out for 5, 10, and 20 minutes; seeds soaked but not sonicated served as a control.
At the end, the level of lipid peroxidation was determined in the seeds by the level of malonic
dialdehyde (MDA) concentration, OMB registration of 2,4 – denitrophenylhydrazones
(2,4 – DNPH), cysteine proteinase activity and gene expression (CP). Results. The experiments
revealed the dependence of the studied parameters on the time of ultrasonic exposure.
An increase in MDA content in germinating wheat seeds was shown after ultrasonic
exposure. The content of 2,4 – DNPH had a wave-like dynamics, did not statistically significantly
change in germinating seeds exposed to ultrasound for 5 min, increased in samples
that were exposed to ultrasound for 10 min, and decreased in seeds treated with ultrasound
for 20 minutes. The activity of the studied proteinase was higher in the samples after 5 minutes
of ultrasonic treatment and lower than the control values in the seeds after 20 minutes
of sonication. Gene expression (SR) was higher in germinating seeds subjected to
5–10 minutes ultrasonic treatment, followed by a fall below the control. Conclusions.
The intensification of LPO processes, the wave-like dynamics of POM and the primary
activation of proteolysis with subsequent inhibition in germinating wheat seeds after ultrasound
exposure were established; protein homeostasis is more tolerant to the action of ROS
compared to lipids. |
| format |
article |
| author |
S.S. Tarasov A.P. Veselov |
| author_facet |
S.S. Tarasov A.P. Veselov |
| author_sort |
S.S. Tarasov |
| title |
Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| title_short |
Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| title_full |
Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| title_fullStr |
Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| title_full_unstemmed |
Oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| title_sort |
oxidative homeostasis of germinating wheat seeds depending on the ultrasonic effect’s duration |
| publisher |
Penza State University Publishing House |
| publishDate |
2021 |
| url |
https://doaj.org/article/12a4379e55644e0c84cf9a4a9bf13385 |
| work_keys_str_mv |
AT sstarasov oxidativehomeostasisofgerminatingwheatseedsdependingontheultrasoniceffectsduration AT apveselov oxidativehomeostasisofgerminatingwheatseedsdependingontheultrasoniceffectsduration |
| _version_ |
1718415089967562752 |