SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS

Abstract. The comparative analysis of phenotypical and functional features of dendritic cells (DCs), generated in presence of GM–CSF and IFNα from blood monocytes of patients with malignant gliomas (MG) and healthy donors, was carried out in this research. The potential value of the DC–based immunot...

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Autores principales:	O. Yu. Leplina, M. A. Tihonova, Yu. P. Kozlov, V. V. Stupak, S. D. Nikonov, A. A. Ostanin, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	dendritic cells anti-tumor response immunotherapy malignant gliomas Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/12a6b9178eab432496ab6d34904cb0cc
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spelling	oai:doaj.org-article:12a6b9178eab432496ab6d34904cb0cc2021-11-18T08:03:42ZSEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS1563-06252313-741X10.15789/1563-0625-2005-4-365-374https://doaj.org/article/12a6b9178eab432496ab6d34904cb0cc2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/484https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The comparative analysis of phenotypical and functional features of dendritic cells (DCs), generated in presence of GM–CSF and IFNα from blood monocytes of patients with malignant gliomas (MG) and healthy donors, was carried out in this research. The potential value of the DC–based immunotherapy in the induction of anti–tumor response in patients with MG was also examined. Our results show that within generated DCs of healthy donors 90 and 52% cells expressed correspondingly HLA–DR and CD86, only 17–18% cells were CD14+monocytes, whereas 38% cells exhibited the phenotype of mature CD83+ dendritic cells. The both monocyte conditioned medium (MCM, 30% v/v) and Leukinferon® (250 IU of IFNα) were comparably efficient as maturation–induced stimuli. Despite monocyte’s disturbances in malignant gliomas, the analogous population of DCs was efficiently generated in all examined patients with MG. However, the percentage of mature CD83+DCs was significantly decreased compared to that in healthy donors (24 vs 38%), and these data strongly suggest the delay maturation of DCs in MG. Nevertheless the patient’s DCs showed the allostimulatory activity, comparable with healthy donor’s DCs, and 52–62% cells maintained the ability for the receptor–dependent en–docytosis. Moreover, the patient’s DCs effectively presented bacterial and tumor–associated antigens (TAA). Immunotherapy with autologous DCs allowed to induce the TAA–specific immune reactions, both in skin test in vivo and in vitro, in 50% patients with MG. (Med. Immunol., 2005, vol.7, № 4, pp. 365–374)O. Yu. LeplinaM. A. TihonovaYu. P. KozlovV. V. StupakS. D. NikonovA. A. OstaninE. R. ChernykhSPb RAACIarticledendritic cellsanti-tumor responseimmunotherapymalignant gliomasImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 7, Iss 4, Pp 365-374 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	dendritic cells anti-tumor response immunotherapy malignant gliomas Immunologic diseases. Allergy RC581-607
spellingShingle	dendritic cells anti-tumor response immunotherapy malignant gliomas Immunologic diseases. Allergy RC581-607 O. Yu. Leplina M. A. Tihonova Yu. P. Kozlov V. V. Stupak S. D. Nikonov A. A. Ostanin E. R. Chernykh SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS
description	Abstract. The comparative analysis of phenotypical and functional features of dendritic cells (DCs), generated in presence of GM–CSF and IFNα from blood monocytes of patients with malignant gliomas (MG) and healthy donors, was carried out in this research. The potential value of the DC–based immunotherapy in the induction of anti–tumor response in patients with MG was also examined. Our results show that within generated DCs of healthy donors 90 and 52% cells expressed correspondingly HLA–DR and CD86, only 17–18% cells were CD14+monocytes, whereas 38% cells exhibited the phenotype of mature CD83+ dendritic cells. The both monocyte conditioned medium (MCM, 30% v/v) and Leukinferon® (250 IU of IFNα) were comparably efficient as maturation–induced stimuli. Despite monocyte’s disturbances in malignant gliomas, the analogous population of DCs was efficiently generated in all examined patients with MG. However, the percentage of mature CD83+DCs was significantly decreased compared to that in healthy donors (24 vs 38%), and these data strongly suggest the delay maturation of DCs in MG. Nevertheless the patient’s DCs showed the allostimulatory activity, comparable with healthy donor’s DCs, and 52–62% cells maintained the ability for the receptor–dependent en–docytosis. Moreover, the patient’s DCs effectively presented bacterial and tumor–associated antigens (TAA). Immunotherapy with autologous DCs allowed to induce the TAA–specific immune reactions, both in skin test in vivo and in vitro, in 50% patients with MG. (Med. Immunol., 2005, vol.7, № 4, pp. 365–374)
format	article
author	O. Yu. Leplina M. A. Tihonova Yu. P. Kozlov V. V. Stupak S. D. Nikonov A. A. Ostanin E. R. Chernykh
author_facet	O. Yu. Leplina M. A. Tihonova Yu. P. Kozlov V. V. Stupak S. D. Nikonov A. A. Ostanin E. R. Chernykh
author_sort	O. Yu. Leplina
title	SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS
title_short	SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS
title_full	SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS
title_fullStr	SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS
title_full_unstemmed	SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS
title_sort	semi–mature dendritic cells as a potential basis for the induction of anti–tumor response in patients with malignant gliomas
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/12a6b9178eab432496ab6d34904cb0cc
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SEMI–MATURE DENDRITIC CELLS AS A POTENTIAL BASIS FOR THE INDUCTION OF ANTI–TUMOR RESPONSE IN PATIENTS WITH MALIGNANT GLIOMAS

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