Novel combination of CRISPR-based gene drives eliminates resistance and localises spread

Abstract Invasive species are among the major driving forces behind biodiversity loss. Gene drive technology may offer a humane, efficient and cost-effective method of control. For safe and effective deployment it is vital that a gene drive is both self-limiting and can overcome evolutionary resista...

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Autores principales:	Nicky R. Faber, Gus R. McFarlane, R. Chris Gaynor, Ivan Pocrnic, C. Bruce A. Whitelaw, Gregor Gorjanc
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12ac74c584cf4cc2828c4d6d24a4730a
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spelling	oai:doaj.org-article:12ac74c584cf4cc2828c4d6d24a4730a2021-12-02T13:35:04ZNovel combination of CRISPR-based gene drives eliminates resistance and localises spread10.1038/s41598-021-83239-42045-2322https://doaj.org/article/12ac74c584cf4cc2828c4d6d24a4730a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83239-4https://doaj.org/toc/2045-2322Abstract Invasive species are among the major driving forces behind biodiversity loss. Gene drive technology may offer a humane, efficient and cost-effective method of control. For safe and effective deployment it is vital that a gene drive is both self-limiting and can overcome evolutionary resistance. We present HD-ClvR in this modelling study, a novel combination of CRISPR-based gene drives that eliminates resistance and localises spread. As a case study, we model HD-ClvR in the grey squirrel (Sciurus carolinensis), which is an invasive pest in the UK and responsible for both biodiversity and economic losses. HD-ClvR combats resistance allele formation by combining a homing gene drive with a cleave-and-rescue gene drive. The inclusion of a self-limiting daisyfield gene drive allows for controllable localisation based on animal supplementation. We use both randomly mating and spatial models to simulate this strategy. Our findings show that HD-ClvR could effectively control a targeted grey squirrel population, with little risk to other populations. HD-ClvR offers an efficient, self-limiting and controllable gene drive for managing invasive pests.Nicky R. FaberGus R. McFarlaneR. Chris GaynorIvan PocrnicC. Bruce A. WhitelawGregor GorjancNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicky R. Faber Gus R. McFarlane R. Chris Gaynor Ivan Pocrnic C. Bruce A. Whitelaw Gregor Gorjanc Novel combination of CRISPR-based gene drives eliminates resistance and localises spread
description	Abstract Invasive species are among the major driving forces behind biodiversity loss. Gene drive technology may offer a humane, efficient and cost-effective method of control. For safe and effective deployment it is vital that a gene drive is both self-limiting and can overcome evolutionary resistance. We present HD-ClvR in this modelling study, a novel combination of CRISPR-based gene drives that eliminates resistance and localises spread. As a case study, we model HD-ClvR in the grey squirrel (Sciurus carolinensis), which is an invasive pest in the UK and responsible for both biodiversity and economic losses. HD-ClvR combats resistance allele formation by combining a homing gene drive with a cleave-and-rescue gene drive. The inclusion of a self-limiting daisyfield gene drive allows for controllable localisation based on animal supplementation. We use both randomly mating and spatial models to simulate this strategy. Our findings show that HD-ClvR could effectively control a targeted grey squirrel population, with little risk to other populations. HD-ClvR offers an efficient, self-limiting and controllable gene drive for managing invasive pests.
format	article
author	Nicky R. Faber Gus R. McFarlane R. Chris Gaynor Ivan Pocrnic C. Bruce A. Whitelaw Gregor Gorjanc
author_facet	Nicky R. Faber Gus R. McFarlane R. Chris Gaynor Ivan Pocrnic C. Bruce A. Whitelaw Gregor Gorjanc
author_sort	Nicky R. Faber
title	Novel combination of CRISPR-based gene drives eliminates resistance and localises spread
title_short	Novel combination of CRISPR-based gene drives eliminates resistance and localises spread
title_full	Novel combination of CRISPR-based gene drives eliminates resistance and localises spread
title_fullStr	Novel combination of CRISPR-based gene drives eliminates resistance and localises spread
title_full_unstemmed	Novel combination of CRISPR-based gene drives eliminates resistance and localises spread
title_sort	novel combination of crispr-based gene drives eliminates resistance and localises spread
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/12ac74c584cf4cc2828c4d6d24a4730a
work_keys_str_mv	AT nickyrfaber novelcombinationofcrisprbasedgenedriveseliminatesresistanceandlocalisesspread AT gusrmcfarlane novelcombinationofcrisprbasedgenedriveseliminatesresistanceandlocalisesspread AT rchrisgaynor novelcombinationofcrisprbasedgenedriveseliminatesresistanceandlocalisesspread AT ivanpocrnic novelcombinationofcrisprbasedgenedriveseliminatesresistanceandlocalisesspread AT cbruceawhitelaw novelcombinationofcrisprbasedgenedriveseliminatesresistanceandlocalisesspread AT gregorgorjanc novelcombinationofcrisprbasedgenedriveseliminatesresistanceandlocalisesspread
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Novel combination of CRISPR-based gene drives eliminates resistance and localises spread

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