HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model

Yinian Zhang,1,2,* Hua-Teng Dong,3,* Lei Duan,1,2 Liang Niu,1,2 Guo-Qiang Yuan,1,2 Jun-Qiang Dai,1,2 Bo-Ru Hou,1,2 Ya-Wen Pan1,2 1Department of Neurosurgery and Laboratory of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730030, People’s Republic of China; 2Institute of Neurol...

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Auteurs principaux:	Zhang YN, Dong HT, Duan L, Niu L, Yuan GQ, Dai JQ, Hou BR, Pan YW
Format:	article
Langue:	EN
Publié:	Dove Medical Press 2019
Sujets:	Epilepsy HDAC4 Gene silencing GABA receptor GABA transporter Seizures Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Accès en ligne:	https://doaj.org/article/12ad28ff00d642d0974aeee2a3a85085
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spelling	oai:doaj.org-article:12ad28ff00d642d0974aeee2a3a850852021-12-02T04:28:37ZHDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model1178-2021https://doaj.org/article/12ad28ff00d642d0974aeee2a3a850852019-02-01T00:00:00Zhttps://www.dovepress.com/hdac4-gene-silencing-alleviates-epilepsy-by-inhibition-of-gaba-in-a-ra-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yinian Zhang,1,2,* Hua-Teng Dong,3,* Lei Duan,1,2 Liang Niu,1,2 Guo-Qiang Yuan,1,2 Jun-Qiang Dai,1,2 Bo-Ru Hou,1,2 Ya-Wen Pan1,2 1Department of Neurosurgery and Laboratory of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730030, People’s Republic of China; 2Institute of Neurology, Lanzhou University, Lanzhou 730030, People’s Republic of China; 3Department of Pediatric Neurology, Gansu Provincial Maternity and Child-Care Hospital, Lanzhou 730050, People’s Republic of China *These authors contributed equally to this work Objectives: Despite the availability of effective antiepileptic drugs, epileptic patients still suffer from intractable seizures and adverse events. Better control of both seizures and fewer side effects is needed in order to enhance the patient’s quality of life. We performed the present study with an attempt to explore the effect that HDAC4 gene silencing would have on epilepsy simulated by model rats. Furthermore, the study made additional analysis on the relativity of the HDAC4 gene in regard to its relationship with the gamma-aminobutyric acid (GABA) signaling pathway. Materials and methods: Tremor rats were prepared in order to establish the epilepsy model. The rats would go on to be treated with si-HDAC4 in order to identify roles of the HDAC4 in levels of GABAARα1, GABAARα4, GAD65, GAT-1, and GAT-3. Finally, both electroencephalogram behavior and cognitive function of the rats following the treatment of si-HDAC4 were observed. Results: Levels of the GABAARα1 and GABAARα4 showed an evident increase, while GAD65, GAT-1, and GAT-3 displayed a decline in the epilepsy rats treated with the aforementioned si-HDAC4 when compared with the epilepsy rats. After injection of si-HDAC4, the epilepsy rats presented with a reduction in seizure degree, latency and duration of seizure, amount of scattered epileptic waves, and occurrence of epilepsy, with an improvement in their cognitive function. Conclusion: The study highlighted the role that HDAC4 gene silencing played in easing the cases of epilepsy found in the model rats. This was shown to have occurred through the upregulation of both GABAARα1 and GABAARα4 levels, as well as in the downregulation of GAD65, GAT-1, and GAT-3 levels. The evidence provided shows that the HDAC4 gene is likely to present as a new objective in further experimentation in the treatment of epilepsy. Keywords: epilepsy, HDAC4, gene silencing, GABA receptor, GABA transporter, seizuresZhang YNDong HTDuan LNiu LYuan GQDai JQHou BRPan YWDove Medical PressarticleEpilepsyHDAC4Gene silencingGABA receptorGABA transporterSeizuresNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 405-416 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Epilepsy HDAC4 Gene silencing GABA receptor GABA transporter Seizures Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Epilepsy HDAC4 Gene silencing GABA receptor GABA transporter Seizures Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Zhang YN Dong HT Duan L Niu L Yuan GQ Dai JQ Hou BR Pan YW HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model
description	Yinian Zhang,1,2,* Hua-Teng Dong,3,* Lei Duan,1,2 Liang Niu,1,2 Guo-Qiang Yuan,1,2 Jun-Qiang Dai,1,2 Bo-Ru Hou,1,2 Ya-Wen Pan1,2 1Department of Neurosurgery and Laboratory of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730030, People’s Republic of China; 2Institute of Neurology, Lanzhou University, Lanzhou 730030, People’s Republic of China; 3Department of Pediatric Neurology, Gansu Provincial Maternity and Child-Care Hospital, Lanzhou 730050, People’s Republic of China *These authors contributed equally to this work Objectives: Despite the availability of effective antiepileptic drugs, epileptic patients still suffer from intractable seizures and adverse events. Better control of both seizures and fewer side effects is needed in order to enhance the patient’s quality of life. We performed the present study with an attempt to explore the effect that HDAC4 gene silencing would have on epilepsy simulated by model rats. Furthermore, the study made additional analysis on the relativity of the HDAC4 gene in regard to its relationship with the gamma-aminobutyric acid (GABA) signaling pathway. Materials and methods: Tremor rats were prepared in order to establish the epilepsy model. The rats would go on to be treated with si-HDAC4 in order to identify roles of the HDAC4 in levels of GABAARα1, GABAARα4, GAD65, GAT-1, and GAT-3. Finally, both electroencephalogram behavior and cognitive function of the rats following the treatment of si-HDAC4 were observed. Results: Levels of the GABAARα1 and GABAARα4 showed an evident increase, while GAD65, GAT-1, and GAT-3 displayed a decline in the epilepsy rats treated with the aforementioned si-HDAC4 when compared with the epilepsy rats. After injection of si-HDAC4, the epilepsy rats presented with a reduction in seizure degree, latency and duration of seizure, amount of scattered epileptic waves, and occurrence of epilepsy, with an improvement in their cognitive function. Conclusion: The study highlighted the role that HDAC4 gene silencing played in easing the cases of epilepsy found in the model rats. This was shown to have occurred through the upregulation of both GABAARα1 and GABAARα4 levels, as well as in the downregulation of GAD65, GAT-1, and GAT-3 levels. The evidence provided shows that the HDAC4 gene is likely to present as a new objective in further experimentation in the treatment of epilepsy. Keywords: epilepsy, HDAC4, gene silencing, GABA receptor, GABA transporter, seizures
format	article
author	Zhang YN Dong HT Duan L Niu L Yuan GQ Dai JQ Hou BR Pan YW
author_facet	Zhang YN Dong HT Duan L Niu L Yuan GQ Dai JQ Hou BR Pan YW
author_sort	Zhang YN
title	HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model
title_short	HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model
title_full	HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model
title_fullStr	HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model
title_full_unstemmed	HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model
title_sort	hdac4 gene silencing alleviates epilepsy by inhibition of gaba in a rat model
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/12ad28ff00d642d0974aeee2a3a85085
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HDAC4 gene silencing alleviates epilepsy by inhibition of GABA in a rat model

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